FastQCFastQC Report
Sun 21 May 2023
EGAF00007847776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59221
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCAAGCGTTA30435.138379966565914No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA22543.806082301886155No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCAAGCGTTA18073.051282484253896No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA16082.7152530352408775No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT16022.7051214940646053No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCAAGCGTTA15362.593674541125614No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA14712.4839161783826684No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT13422.2660880430928216No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT13352.2542679117205044No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT13292.2441363705442323No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA12202.0600800391752925No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT11381.921615643099576No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG11201.8912210195707606No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA10751.815234460748721No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCAAGCGTTA10711.8084800999645396No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9081.5332398980091522No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA7681.296837270562807No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA7601.2833285489944446No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT7501.2664426470339913No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7461.25968828624981No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7281.2292936627209943No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7111.2005876293882238No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6451.0891406764492324No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT6191.0452373313520542No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT5920.9996453960588304No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5850.9878252646865131No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5760.9726279529221054No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5670.9574306411576975No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5530.933790378413063No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5310.8966413941000659No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA5280.89157562351193No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT5200.8780669019435673No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT5160.8713125411593861No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4950.8358521470424343No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT4930.8324749666503437No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4700.7936373921413011No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4660.7868830313571199No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT4330.7311595548876243No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4070.6872562097904459No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3780.6382870941051316No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT3740.6315327333209503No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3580.6045152901842252No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3480.587629388223772No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3470.5859407980277266No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3360.5673663058712282No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3280.5538575843028655No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT3190.5386602725384576No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3160.5335945019503217No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3090.5217743705780045No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3080.5200857803819592No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2930.49475692744127925No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2920.49306833724523397No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2900.4896911568531433No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2740.4626737137164182No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2610.4407220411678289No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2570.43396768038364775No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2560.43227909018760236No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2540.4289019097955117No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2510.42383613920737573No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2420.40863882744296787No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2410.4069502372469226No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2370.40019587646274124No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2230.37655561371810675No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT2180.3681126627378801No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2130.3596697117576535No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1960.33096367842488306No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1920.32420931764070177No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1860.31407777646442986No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1860.31407777646442986No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1840.31070059607233924No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1750.2955032843079313No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1700.2870603333277047No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA1620.2735516117593422No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1600.2701744313672515No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1570.2651086607791155No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1570.2651086607791155No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1470.24822275881866232No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1440.24315698823052634No Hit
TCGACCGAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1410.23809121764239036No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1350.22795967646611842No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1200.2026308235254386No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1200.2026308235254386No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1160.19587646274125733No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1110.1874335117610307No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1110.1874335117610307No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1110.1874335117610307No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1100.1857449215649854No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1100.1857449215649854No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1100.1857449215649854No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1060.1789905607808041No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGACAAGCGTTG990.16717042940848686No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA950.16041606862430557No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA910.15366170784012428No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT870.146907347055943No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC840.14184157646780704No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.1367758058796711No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA810.1367758058796711No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTG790.1333986254875804No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA790.1333986254875804No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.13171003529153508No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT750.12664426470339912No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA740.1249556745073538No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA740.1249556745073538No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG720.12157849411526317No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA710.11988990391921783No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA690.1165127235271272No Hit
TCGACCGAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT670.11313554313503654No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT670.11313554313503654No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC650.10975836274294591No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA650.10975836274294591No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC630.10638118235085528No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA620.10469259215480996No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC620.10469259215480996No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA600.1013154117627193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)