FastQCFastQC Report
Sun 21 May 2023
EGAF00007847780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24873
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG16826.762352751980058No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG12364.969243758292124No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7082.84646001688578No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5752.31174365778153No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5752.31174365778153No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA4921.978048486310457No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4541.8252723837092428No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4431.7810477224299441No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4021.6162103485707395No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3871.5559039922807862No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3521.4151891609375629No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2981.198086278293732No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2831.1377799220037792No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2811.1297390744984521No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2671.0734531419611626No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2430.976962971897238No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA2360.9488200056285933No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2320.9327383106179391No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2320.9327383106179391No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2230.8965544968439673No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2190.8804728018333133No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA2180.8764523780806497No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC2150.8643911068226592No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2090.8402685643066778No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2090.8402685643066778No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC2080.8362481405540144No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT1930.7759417842640615No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1910.7679009367587344No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1890.7598600892534073No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1860.7477988179954167No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1810.727696699232099No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1780.7156354279741085No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT1710.6874924617054637No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1700.6834720379528003No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1650.6633699191894825No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1580.6352269529208379No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1510.6070839866521931No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1460.5869818678888755No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1450.582961444136212No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1440.5789410203835484No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1420.5709001728782214No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1390.5588389016202308No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1330.5347163591042495No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1320.5306959353515861No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1310.5266755115989225No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1300.522655087846259No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1300.522655087846259No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1220.4904916978249508No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1210.4864712740722872No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1210.4864712740722872No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1150.462348731556306No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1140.45832830780364253No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1140.45832830780364253No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1120.45028746029831546No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1110.4462670365456519No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1110.4462670365456519No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1100.4422466127929884No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1070.43018534153499777No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1070.43018534153499777No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC1070.43018534153499777No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1060.42616491778233423No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1060.42616491778233423No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1050.42214449402967075No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1050.42214449402967075No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1030.41410364652434367No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1010.4060627990190166No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1000.4020423752663531No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1000.4020423752663531No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT990.3980219515136895No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT960.38596068025569896No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCT940.3779198327503719No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT920.36987898524504487No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA890.35781771398705425No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC880.3537972902343907No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC870.3497768664817272No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA870.3497768664817272No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC830.3336951714710731No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA810.325654323965746No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT750.30153178144976484No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA750.30153178144976484No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT740.2975113576971013No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT740.2975113576971013No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT740.2975113576971013No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT720.2894705101917742No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.2854500864391107No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG700.28142966268644715No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA690.27740923893378366No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC670.2693683914284566No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCT670.2693683914284566No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA660.26534796767579305No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT650.2613275439231295No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA650.2613275439231295No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCT640.257307120170466No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT640.257307120170466No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC640.257307120170466No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT640.257307120170466No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT630.25328669641780244No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT630.25328669641780244No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCT610.2452458489124754No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT610.2452458489124754No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA600.24122542515981185No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA600.24122542515981185No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT600.24122542515981185No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT590.2372050014071483No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG580.23318457765448478No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCT580.23318457765448478No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA580.23318457765448478No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT580.23318457765448478No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG560.22514373014915773No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT560.22514373014915773No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACC540.21710288264383065No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT520.2090620351385036No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA520.2090620351385036No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC510.20504161138584007No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT510.20504161138584007No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG500.20102118763317656No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC500.20102118763317656No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG490.19700076388051302No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC490.19700076388051302No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC480.19298034012784948No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT480.19298034012784948No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTCGATACCCGCACC480.19298034012784948No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT480.19298034012784948No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT470.18895991637518594No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA470.18895991637518594No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA460.18493949262252243No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT450.1809190688698589No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT450.1809190688698589No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG440.17689864511719536No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA430.17287822136453182No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT420.1688577976118683No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCTGGTGTTA400.16081695010654123No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA390.1567965263538777No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT380.1527761026012142No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA380.1527761026012142No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG380.1527761026012142No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT360.1447352550958871No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC350.14071483134322357No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACC350.14071483134322357No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACC340.13669440759056006No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA340.13669440759056006No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCTGGTGTTA330.13267398383789653No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT330.13267398383789653No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT330.13267398383789653No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA320.128653560085233No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT320.128653560085233No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA310.12463313633256945No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT290.11659228882724239No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT290.11659228882724239No Hit
TCGACCGAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT280.11257186507457886No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA280.11257186507457886No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA270.10855144132191533No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC270.10855144132191533No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC260.1045310175692518No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT260.1045310175692518No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA260.1045310175692518No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT250.10051059381658828No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACT250.10051059381658828No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA250.10051059381658828No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)