FastQCFastQC Report
Sun 21 May 2023
EGAF00007847782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32907
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA12793.8867110341264777No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11593.5220469808855257No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8412.555687239797004No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7802.3703163460661867No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6401.9448749506184095No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6351.929680615066703No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6211.8871364755219253No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6011.8263591333151004No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5581.6956878475704258No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4961.5072780867292672No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4921.4951226182879023No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4871.479928282736196No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4451.3522958641018628No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3991.2125079770261646No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3981.2094691099158232No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3951.2003525085847997No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3781.148691767708998No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3511.066642355729784No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3120.9481265384264745No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2920.8873491962196494No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2890.8782325948886255No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2730.8296107211231653No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2610.7931443157990701No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2560.7779499802473637No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2550.7749111131370225No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2480.7536390433646336No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2460.7475613091439511No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2400.7293281064819035No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2370.7202115051508797No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2360.7171726380405385No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2340.711094903819856No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2300.6989394353784909No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2280.6928617011578084No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2260.6867839669371258No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2240.6807062327164433No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2230.677667365606102No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2180.6624730300543957No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2120.6442398273923481No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2110.6412009602820069No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2030.6168900233992768No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1990.6047345549579116No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1960.5956179536268879No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1870.5682681496338166No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA1830.5561126811924514No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1830.5561126811924514No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1750.5318017443097213No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1700.516607408758015No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA1680.5105296745373326No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1580.4801410034339198No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1570.47710213632357856No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1560.47406326921323727No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1550.47102440210289603No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1460.4436745981098247No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1440.43759686388914215No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1370.41632479411675327No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1360.41328592700641203No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1350.4102470598960708No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1350.4102470598960708No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1340.40720819278572945No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA1270.3859361230133406No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1270.3859361230133406No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1260.3828972559029994No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1250.3798583887926581No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1230.37378065457197557No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1220.37074178746163433No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1210.36770292035129304No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1210.36770292035129304No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1180.3585863190202693No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1130.3433919834685629No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1110.3373142492478804No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1080.32819764791685657No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1080.32819764791685657No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1080.32819764791685657No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA1050.3190810465858328No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.3160421794754915No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1030.3130033123651503No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1000.3038867110341265No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA970.2947701097031027No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT960.29173124259276145No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT960.29173124259276145No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA950.28869237548242016No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT950.28869237548242016No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT940.28565350837207887No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT920.2795757741513964No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT910.2765369070410551No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC910.2765369070410551No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT900.2734980399307138No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA850.2583037043790075No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT810.24614823593764246No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC800.2431093688273012No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA790.2400705017169599No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT770.2339927674962774No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC750.22791503327559484No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA730.22183729905491234No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.21272069772388852No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA690.20968183061354728No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT640.19448749506184093No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA630.1914486279514997No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT630.1914486279514997No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT620.1884097608411584No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.18537089373081717No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT610.18537089373081717No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT570.1732154252894521No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC570.1732154252894521No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT560.17017655817911082No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT550.16713769106876955No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA540.16409882395842829No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT540.16409882395842829No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC530.16105995684808702No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC530.16105995684808702No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA530.16105995684808702No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC520.15802108973774576No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC500.15194335551706326No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA480.14586562129638073No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA480.14586562129638073No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACT470.14282675418603943No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC470.14282675418603943No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC470.14282675418603943No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT460.1397878870756982No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA450.1367490199653569No Hit
TCGACCGAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT450.1367490199653569No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT450.1367490199653569No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA440.13371015285501567No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT440.13371015285501567No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT440.13371015285501567No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC440.13371015285501567No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT440.13371015285501567No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA440.13371015285501567No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACT430.13067128574467438No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT420.12763241863433314No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA420.12763241863433314No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT420.12763241863433314No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA410.12459355152399185No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC410.12459355152399185No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT410.12459355152399185No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT410.12459355152399185No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA410.12459355152399185No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT410.12459355152399185No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT410.12459355152399185No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA410.12459355152399185No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT400.1215546844136506No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA380.11547695019296807No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT380.11547695019296807No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT370.11243808308262679No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA370.11243808308262679No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT370.11243808308262679No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT360.10939921597228554No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA360.10939921597228554No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT360.10939921597228554No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC360.10939921597228554No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGGGCAAGCGTTA360.10939921597228554No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA350.10636034886194426No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACC340.10332148175160301No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT340.10332148175160301No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT340.10332148175160301No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT340.10332148175160301No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA340.10332148175160301No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC340.10332148175160301No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC330.10028261464126173No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT330.10028261464126173No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA330.10028261464126173No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCTGGCGTTA330.10028261464126173No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT330.10028261464126173No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA330.10028261464126173No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT330.10028261464126173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)