FastQCFastQC Report
Sun 21 May 2023
EGAF00007847786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31523
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13144.1683849887383815No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11823.7496431177235667No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6412.0334359039431527No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6312.001713034926879No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6301.9985407480252515No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5981.8970275671731751No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4901.5544205817974177No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4781.5163531389778893No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4571.4497351140437142No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4261.3513942200932652No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4231.341877359388383No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4191.3291882117818736No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4091.2974653427655998No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3911.2403641785363069No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3721.1800907274053867No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3571.1325064238809757No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3511.1134727024712114No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3501.1103004155695841No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3401.0785775465533103No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3150.9992703740126258No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3120.9897535133077435No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3050.9675475049963519No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2990.9485137835865876No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2790.8850680455540398No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2790.8850680455540398No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2750.8723788979475304No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2730.8660343241442756No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2710.8596897503410208No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2500.7930717254068458No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2420.7676934301938267No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2380.7550042825873172No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2360.7486597087840624No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2290.7264537004726708No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2180.6915585445547695No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2140.67886939694826No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2070.6566633886368684No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1970.6249405196205945No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1960.6217682327189671No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1860.5900453637026933No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1860.5900453637026933No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1840.5837007898994385No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1800.571011642292929No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1760.5583224946864194No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1740.5519779208831647No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1710.5424610601782824No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1610.5107381911620087No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1600.5075659042603814No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1600.5075659042603814No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1600.5075659042603814No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1600.5075659042603814No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1600.5075659042603814No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1580.5012213304571266No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1580.5012213304571266No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1560.49487675665387176No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1500.4758430352441075No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1480.46949846144085267No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1450.45998160073597055No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1440.4568093138343432No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1380.4377755924245789No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1370.43460330552295146No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1310.4155695841131872No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1310.4155695841131872No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1270.40288043650667765No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1250.3965358627034229No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1240.39336357580179554No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1220.3870190019985407No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1210.38384671509691337No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1180.3743298543920312No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1050.33309012467087523No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1030.32674555086762047No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1020.32357326396599306No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1010.32040097706436566No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1000.3172286901627383No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA980.31088411635948354No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA980.31088411635948354No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA970.3077118294578562No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA950.3013672556546014No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT940.298194968752974No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT930.29502268185134667No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT920.29185039494971926No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC920.29185039494971926No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT920.29185039494971926No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT910.28867810804809185No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC900.2855058211464645No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT900.2855058211464645No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT870.27598896044158233No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA860.272816673539955No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA850.26964438663832757No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA840.26647209973670016No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT840.26647209973670016No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC830.2632998128350728No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT810.25695523903181805No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT810.25695523903181805No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA790.2506106652285633No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT770.24426609142530847No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT760.24109380452368112No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA740.23474923072042633No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA740.23474923072042633No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT720.2284046569171716No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT700.22206008311391684No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC700.22206008311391684No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.22206008311391684No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA690.21888779621228946No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA680.21571550931066205No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT670.21254322240903464No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC650.2061986486057799No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA640.2030263617041525No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC640.2030263617041525No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA640.2030263617041525No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA630.19985407480252515No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA590.1871649271960156No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT580.18399264029438822No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.18399264029438822No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG570.18082035339276084No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT560.17764806649113346No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.17764806649113346No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.17764806649113346No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT550.17447577958950608No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.16495891888462394No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA520.16495891888462394No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC520.16495891888462394No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC500.15861434508136915No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA500.15861434508136915No Hit
TCGACCGAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT500.15861434508136915No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG480.1522697712781144No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT460.14592519747485963No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC460.14592519747485963No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT460.14592519747485963No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC440.13958062367160484No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC430.1364083367699775No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA430.1364083367699775No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT430.1364083367699775No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACT430.1364083367699775No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA420.13323604986835008No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT420.13323604986835008No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT410.1300637629667227No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA410.1300637629667227No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACT400.12689147606509535No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT400.12689147606509535No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT400.12689147606509535No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT390.12371918916346794No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC390.12371918916346794No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA390.12371918916346794No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC390.12371918916346794No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA390.12371918916346794No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACT380.12054690226184056No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT380.12054690226184056No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA370.11737461536021317No Hit
TCGACCGAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT370.11737461536021317No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA350.11103004155695842No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT350.11103004155695842No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCAAGCGTTG350.11103004155695842No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT350.11103004155695842No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTA340.10785775465533103No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA340.10785775465533103No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT340.10785775465533103No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT340.10785775465533103No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA330.10468546775370365No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG330.10468546775370365No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT330.10468546775370365No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAACACGTAAGGTGCGAGCGTTG330.10468546775370365No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT320.10151318085207625No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA320.10151318085207625No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)