FastQCFastQC Report
Sun 21 May 2023
EGAF00007847787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31523
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12714.031976651968404No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11673.7020588141991566No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6031.912889001681312No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5631.7859975256162168No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5381.7066903530755324No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5211.6527614757478666No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5041.5988325984202012No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4761.5100085651746344No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4641.4719411223551058No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4561.4465628271420867No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4451.4116676712241856No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4311.367255654601402No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4161.3196713510769915No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3931.2467087523395617No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3801.2054690226184055No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3681.167401579798877No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3651.157884719093995No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3491.1071281286679568No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3191.011959521619135No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3030.9612029311930972No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2940.9326523490784506No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2940.9326523490784506No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2920.9263077752751959No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2810.8914126193572948No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2780.8818957586524125No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2720.8628620372426482No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2690.8533451765377662No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2510.7962440123084731No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2510.7962440123084731No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2480.7867271516035911No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2390.7581765694889446No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2390.7581765694889446No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2380.7550042825873172No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2250.7137645528661613No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2250.7137645528661613No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2220.7042476921612791No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2200.6979031183580243No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2110.6693525362433779No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2080.6598356755384958No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2040.6471465279319861No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1960.6217682327189671No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1960.6217682327189671No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1950.6185959458173397No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1930.612251372014085No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1900.6027345113092027No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1890.5995622244075755No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1850.5868730768010658No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1820.5773562160961837No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1640.5202550518668908No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1640.5202550518668908No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1620.5139104780636361No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1620.5139104780636361No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1610.5107381911620087No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1600.5075659042603814No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1560.49487675665387176No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1560.49487675665387176No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1480.46949846144085267No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1470.4663261745392253No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1430.45363702693271585No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1420.4504647400310884No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1370.43460330552295146No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1320.4187418710148146No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1300.4123972972115598No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1280.406052723408305No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1280.406052723408305No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1260.3997081496050503No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1240.39336357580179554No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1210.38384671509691337No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1170.37115756749040385No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1150.36481299368714903No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1130.35846841988389433No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1130.35846841988389433No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1100.34895155917901216No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1070.33943469847413No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1050.33309012467087523No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1000.3172286901627383No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT960.3045395425562288No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC950.3013672556546014No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT890.2823335342448371No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA890.2823335342448371No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA880.2791612473432097No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA850.26964438663832757No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC850.26964438663832757No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA820.2601275259334454No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA800.2537829521301907No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT800.2537829521301907No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA790.2506106652285633No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT790.2506106652285633No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT780.24743837832693588No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA780.24743837832693588No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT750.23792151762205374No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT740.23474923072042633No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT740.23474923072042633No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT730.23157694381879898No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT720.2284046569171716No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA700.22206008311391684No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT700.22206008311391684No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA690.21888779621228946No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT680.21571550931066205No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT680.21571550931066205No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT680.21571550931066205No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA660.2093709355074073No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA650.2061986486057799No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA640.2030263617041525No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.2030263617041525No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT630.19985407480252515No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT610.19350950099927036No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT600.19033721409764298No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.19033721409764298No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC590.1871649271960156No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC580.18399264029438822No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA580.18399264029438822No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC570.18082035339276084No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC550.17447577958950608No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG550.17447577958950608No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT550.17447577958950608No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA540.17130349268787867No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA540.17130349268787867No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT510.16178663198299653No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT510.16178663198299653No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA500.15861434508136915No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA490.15544205817974177No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC490.15544205817974177No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT490.15544205817974177No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA480.1522697712781144No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT480.1522697712781144No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA470.149097484376487No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA470.149097484376487No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT460.14592519747485963No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT450.14275291057323225No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC450.14275291057323225No Hit
TCGACCGAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT450.14275291057323225No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT440.13958062367160484No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC430.1364083367699775No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT430.1364083367699775No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG410.1300637629667227No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACT400.12689147606509535No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA400.12689147606509535No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT400.12689147606509535No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA390.12371918916346794No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT390.12371918916346794No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA390.12371918916346794No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC390.12371918916346794No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC380.12054690226184056No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT380.12054690226184056No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACT370.11737461536021317No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA370.11737461536021317No Hit
TCGACCGAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT370.11737461536021317No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC370.11737461536021317No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT350.11103004155695842No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT350.11103004155695842No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA340.10785775465533103No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCC340.10785775465533103No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT340.10785775465533103No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT330.10468546775370365No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA320.10151318085207625No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC320.10151318085207625No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT320.10151318085207625No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA320.10151318085207625No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)