FastQCFastQC Report
Sun 21 May 2023
EGAF00007847792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43221
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA18104.187779088868837No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA17213.981860669581916No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12172.8157608569908144No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10232.3669049767474144No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT9172.121653825686588No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7281.6843663959649244No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6941.605700932417112No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT6931.6033872423127644No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6611.529349158973647No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6101.4113509636519286No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5991.3859003725041068No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5881.360449781356285No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5821.3465676407302005No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA5441.2586474167649984No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5411.2517063464519562No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5301.2262557553041347No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4981.152217671965017No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4351.0064551953911294No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4341.004141505286782No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4210.9740635339302655No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4100.9486129427824437No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4090.9462992526780962No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4010.9277897318433169No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4000.9254760417389695No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3900.9023391406954953No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3730.863006408921589No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3680.8514379583998519No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3590.8306147474607251No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3590.8306147474607251No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3590.8306147474607251No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3580.8283010573563777No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3570.8259873672520303No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3540.819046296938988No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3520.8144189167302931No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3470.802850466208556No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3380.7820272552694293No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3340.7727724948520395No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3310.7658314245389972No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3190.7380671432868281No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3070.7103028620346591No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2930.6779112005737952No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2870.6640290599477106No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2850.6594016797390158No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2820.6524606094259735No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2630.6085004974433724No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2400.5552856250433817No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2310.5344624141042549No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2140.4951296823303487No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2130.49281599222600125No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2110.48818861201730646No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2100.48587492191295895No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2070.4789338515999167No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1970.4557969505564425No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1810.41877790888688365No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1760.40720945836514655No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1740.40258207815645175No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1720.3979546979477569No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1660.38407255732167234No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1620.37481779690428263No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1580.3655630364868929No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1570.3632493463825455No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1530.3539945859651558No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1520.3516808958608084No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1480.3424261354434187No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1470.34011244533907126No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1470.34011244533907126No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1470.34011244533907126No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1450.33548506513037646No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA1440.333171375026029No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1390.3216029245042919No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1370.31697554429559704No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1360.3146618541912496No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1360.3146618541912496No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA1330.30772078387820734No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1280.29615233335647023No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1270.2938386432521228No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1250.2892112630434279No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1230.2845838828347331No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1230.2845838828347331No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1210.2799565026260383No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1200.2776428125216909No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1180.273015432312996No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1160.2683880521043011No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1150.2660743619999537No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1120.25913329168691146No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1120.25913329168691146No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1080.24987853126952178No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1070.24756484116517435No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1060.24525115106082693No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1050.24293746095647947No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1040.24062377085213205No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1000.23136901043474237No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1000.23136901043474237No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT970.22442794012170011No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT890.2059184192869207No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT870.20129103907822588No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA860.19897734897387845No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT860.19897734897387845No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT850.19666365886953102No Hit
TCGACCGAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT850.19666365886953102No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT840.1943499687651836No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA820.18972258855648874No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT780.18046782813909903No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA770.17815413803475164No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC760.1758404479304042No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT750.17352675782605678No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA740.17121306772170936No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA730.16889937761736193No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT730.16889937761736193No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC720.1665856875130145No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA720.1665856875130145No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA680.1573309270956248No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT680.1573309270956248No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT680.1573309270956248No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA670.1550172369912774No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA660.15270354688692997No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT640.14807616667823512No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA640.14807616667823512No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC630.1457624765738877No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA630.1457624765738877No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA620.14344878646954026No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA610.14113509636519284No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC600.13882140626084544No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT600.13882140626084544No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC600.13882140626084544No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA600.13882140626084544No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC590.136507716156498No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA590.136507716156498No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.136507716156498No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA590.136507716156498No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT590.136507716156498No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.13419402605215056No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT580.13419402605215056No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT580.13419402605215056No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT580.13419402605215056No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC570.13188033594780316No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT570.13188033594780316No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT550.1272529557391083No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT540.12493926563476089No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA530.12262557553041346No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC500.11568450521737118No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.11568450521737118No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCT500.11568450521737118No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT490.11337081511302377No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA490.11337081511302377No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT480.11105712500867634No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT480.11105712500867634No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA480.11105712500867634No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC470.1087434349043289No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG460.10642974479998149No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA460.10642974479998149No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT460.10642974479998149No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA450.10411605469563406No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT450.10411605469563406No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA450.10411605469563406No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA450.10411605469563406No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA440.10180236459128664No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC440.10180236459128664No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT440.10180236459128664No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA440.10180236459128664No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT440.10180236459128664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)