FastQCFastQC Report
Sun 21 May 2023
EGAF00007847793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43221
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA17344.011938640938433No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA17173.972605909164526No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11392.6352930288517156No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10062.327572244973508No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT9812.2697299923648226No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT8601.9897734897387844No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6751.561740820434511No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT6721.5547997501214688No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6411.4830753568866986No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6291.4553110756345295No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6041.397468823025844No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5961.3789593021910644No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5501.2725295573910829No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5441.2586474167649984No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5401.2493926563476088No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA5321.2308831355128294No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5271.2193146849910923No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA5141.1892367136345758No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4671.080493278730247No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4270.98794567455635No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4150.9601813933041807No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3940.911593901112885No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3890.9000254505911478No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3890.9000254505911478No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3760.8699474792346312No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3730.863006408921589No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3650.8444968880868096No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3620.8375558177737673No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3570.8259873672520303No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3530.8167326068346406No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3510.8121052266259458No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3470.802850466208556No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3440.7959093958955138No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3380.7820272552694293No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3270.7565766641216076No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3220.7450082135998705No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3080.7126165521390065No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3060.7079891719303116No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3000.6941070313042271No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2990.6917933411998798No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2930.6779112005737952No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2900.6709701302607529No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2780.6432058490085838No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2630.6085004974433724No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2560.5923046667129405No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2520.5830499062955508No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2360.546030864625992No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2270.5252076536868652No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2260.5228939635825177No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2160.49975706253904356No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2070.4789338515999167No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1920.4442285000347054No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1890.4372874297216631No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1870.4326600495129682No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1840.42571897919992596No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1800.41646421878253626No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1790.41415052867818886No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1750.40489576826079915No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1740.40258207815645175No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1720.3979546979477569No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1710.3956410078434095No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1580.3655630364868929No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1550.35862196617385067No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1540.35630827606950327No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1540.35630827606950327No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1530.3539945859651558No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1500.34705351565211356No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA1460.33779875523472386No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1390.3216029245042919No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1380.31928923439994444No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1320.30540709377385994No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA1320.30540709377385994No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1320.30540709377385994No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1300.3007797135651651No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1280.29615233335647023No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1260.2915249531477754No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1230.2845838828347331No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1220.28227019273038567No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1210.2799565026260383No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1200.2776428125216909No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1170.27070174220864857No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1160.2683880521043011No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1160.2683880521043011No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1140.2637606718956063No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1090.2521922213738692No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1090.2521922213738692No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1080.24987853126952178No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1050.24293746095647947No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.24293746095647947No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1040.24062377085213205No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1030.23831008074778462No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA980.22674163022604754No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC960.2221142500173527No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA950.21980055991300526No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA950.21980055991300526No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT930.21517317970431038No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT930.21517317970431038No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA920.21285948959996298No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA890.2059184192869207No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT890.2059184192869207No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT850.19666365886953102No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA850.19666365886953102No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT830.19203627866083617No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT800.1850952083477939No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT790.18278151824344646No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA750.17352675782605678No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA740.17121306772170936No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC740.17121306772170936No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.16889937761736193No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA720.1665856875130145No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT710.16427199740866708No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA700.16195830730431968No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA690.15964461719997222No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC670.1550172369912774No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC660.15270354688692997No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT660.15270354688692997No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC660.15270354688692997No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA650.15038985678258254No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA640.14807616667823512No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.14807616667823512No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA640.14807616667823512No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA620.14344878646954026No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA600.13882140626084544No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT590.136507716156498No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT590.136507716156498No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC590.136507716156498No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA590.136507716156498No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT590.136507716156498No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.136507716156498No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT590.136507716156498No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG580.13419402605215056No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT570.13188033594780316No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA550.1272529557391083No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT550.1272529557391083No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT550.1272529557391083No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT540.12493926563476089No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGATACCCACGCT530.12262557553041346No Hit
TCGACCGAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT530.12262557553041346No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC520.12031188542606602No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA510.11799819532171861No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.11568450521737118No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT500.11568450521737118No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC500.11568450521737118No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT490.11337081511302377No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA490.11337081511302377No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT490.11337081511302377No Hit
TCATGCCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA490.11337081511302377No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT480.11105712500867634No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA480.11105712500867634No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT470.1087434349043289No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC460.10642974479998149No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT450.10411605469563406No Hit
TCATGCCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA450.10411605469563406No Hit
TCATGCCTTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA450.10411605469563406No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT440.10180236459128664No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT440.10180236459128664No Hit
TCATGCCTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA440.10180236459128664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)