FastQCFastQC Report
Sun 21 May 2023
EGAF00007847794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34239
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA13914.062618651245656No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG12913.770554046555098No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8742.552644644995473No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8302.424136218931628No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA8212.3978504045094775No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7772.2693419784456323No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA7602.219690995648237No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6161.7991179648938345No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5981.7465463360495344No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4961.4486404392651653No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4921.436957855077543No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4771.3931481643739596No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4641.355179765764187No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4501.314290721107509No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4401.2850842606384532No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4261.2441952159817753No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4211.2295919857472473No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4071.1887029410905692No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3761.0981629136364963No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3451.0076228861824235No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3410.9959403019948012No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3370.984257717807179No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3180.928765442915973No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3080.8995589824469172No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2920.852828645696428No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2890.8440667075557113No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2810.8207015391804666No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2790.8148602470866555No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2710.791495078711411No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2650.7739712024299775No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2630.7681299103361664No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2490.7272408656794883No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2340.6834311749759047No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2250.6571453605537545No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2170.6337801921785099No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2130.6220976079908876No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2040.5958117935687374No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2000.5841292093811151No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1990.5812085633342096No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1920.5607640410058705No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1910.5578433949589648No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1840.5373988726306259No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1820.5315575805368148No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1780.5198749963491924No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1780.5198749963491924No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1760.5140337042553813No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1740.5081924121615702No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1630.47606530564560884No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1530.44685884517655305No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1520.4439381991296475No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1510.44101755308274193No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1480.43225561494202525No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1440.4205730307544029No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1430.4176523847074973No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1430.4176523847074973No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1420.41473173866059176No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1400.40889044656678053No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1400.40889044656678053No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1400.40889044656678053No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1340.3913665702853471No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1340.3913665702853471No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1330.38844592423844154No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1310.3826046321446304No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1280.3738426940039137No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1200.3504775256286691No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1200.3504775256286691No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1190.3475568795817635No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1180.3446362335348579No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1130.33003300330033003No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1120.32711235725342447No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1080.31542977306580217No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1070.3125091270188966No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1040.30374718887817986No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1040.30374718887817986No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1030.3008265428312743No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1000.29206460469055756No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG990.28914395864365194No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT980.2862233125967464No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT970.2833026665498408No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT960.28038202050293526No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC950.27746137445602964No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC950.27746137445602964No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT930.2716200823622185No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT930.2716200823622185No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT900.26285814422150183No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA870.2540962060807851No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC870.2540962060807851No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA860.2511755600338795No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA850.2482549139869739No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA840.24533426794006835No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT820.2394929758462572No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT820.2394929758462572No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT810.23657232979935164No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA800.23365168375244602No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT800.23365168375244602No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC750.21904845351791818No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA740.21612780747101262No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT740.21612780747101262No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC740.21612780747101262No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA720.21028651537720144No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC710.20736586933029588No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.20736586933029588No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG690.2015245772364847No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT670.19568328514267355No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT670.19568328514267355No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC660.192762639095768No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC650.1898419930488624No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT650.1898419930488624No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT640.18692134700195684No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA640.18692134700195684No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT640.18692134700195684No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC630.18400070095505125No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT630.18400070095505125No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC610.1781594088612401No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.17523876281433454No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT600.17523876281433454No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT600.17523876281433454No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT590.17231811676742895No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT560.16355617862671223No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT550.16063553257980664No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC540.15771488653290108No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT540.15771488653290108No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA540.15771488653290108No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC530.15479424048599552No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA520.15187359443908993No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA520.15187359443908993No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA510.14895294839218434No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC510.14895294839218434No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC510.14895294839218434No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACT510.14895294839218434No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA490.1431116562983732No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACT480.14019101025146763No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC480.14019101025146763No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC470.13727036420456204No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA460.13434971815765648No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT460.13434971815765648No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA460.13434971815765648No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT450.13142907211075092No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT450.13142907211075092No Hit
TCGACCGAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT450.13142907211075092No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC450.13142907211075092No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT450.13142907211075092No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG440.12850842606384533No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA440.12850842606384533No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCT430.12558778001693974No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC430.12558778001693974No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG410.1197464879231286No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC410.1197464879231286No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT400.11682584187622301No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCT400.11682584187622301No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT400.11682584187622301No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA400.11682584187622301No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT400.11682584187622301No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCT390.11390519582931745No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT390.11390519582931745No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA390.11390519582931745No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACT380.11098454978241187No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT380.11098454978241187No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT380.11098454978241187No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCT380.11098454978241187No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA380.11098454978241187No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT370.10806390373550631No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGATACCCACGCT370.10806390373550631No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA370.10806390373550631No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA360.10514325768860072No Hit
TCGACCGAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT360.10514325768860072No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT350.10222261164169513No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)