FastQCFastQC Report
Sun 21 May 2023
EGAF00007847824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34748
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA15454.446299067572235No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10302.9641993783814895No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10162.9239092897432943No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9042.601588580637735No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8372.40877172786923No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7572.178542649936687No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7052.028893749280534No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5981.7209623575457582No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5531.5914585012087028No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5281.519511914354783No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5201.4964890065615288No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5101.467710371819961No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4941.4216645562334522No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4901.4101531023368252No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4881.4043973753885115No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4871.4015195119143549No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4471.2864049729480833No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4111.182801887878439No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3821.0993438471278922No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3811.0964659836537356No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3701.0648094854380108No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3581.0302751237481294No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3150.9065269943593876No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2910.8374582709796248No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2880.8288246805571544No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2870.8259468170829976No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2790.8029239092897433No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2520.7252215954875101No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2460.7079544146425694No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2350.6762979164268448No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2320.6676643260043744No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2300.6619085990560608No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2300.6619085990560608No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2260.6503971451594337No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2250.6475192816852768No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2230.6417635547369632No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2210.6360078277886496No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2180.6273742373661794No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2100.604351329572925No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2060.5928398756762979No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2030.5842062852538276No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1960.5640612409347301No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1920.552549787038103No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1850.5324047427190054No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1780.5122596983999079No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1750.5036261079774376No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1740.5007482445032807No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1720.4949925175549672No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1650.47484747323586973No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1640.4719696097617129No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1560.4489467019684586No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1550.44606883849430184No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1540.4431909750201451No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1540.4431909750201451No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1500.4316795211235179No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1450.41729020375273396No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1450.41729020375273396No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1440.4144123402785772No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1410.40577874985610685No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1380.3971451594336365No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1380.3971451594336365No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1320.37987797858869576No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1310.377000115114539No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1300.3741222516403822No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1300.3741222516403822No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1290.3712443881662254No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1270.3654886612179118No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1250.35973293426959824No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1230.3539772073212847No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1230.3539772073212847No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1190.3424657534246575No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1180.33958788995050077No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1160.3338321630021872No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1140.3280764360538736No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1130.32519857257971685No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1110.31944284563140324No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1060.3050535282606193No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1060.3050535282606193No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.29929780131230577No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1040.29929780131230577No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1030.29641993783814896No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1000.2877863474156786No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT990.28490848394152185No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA980.28203062046736505No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA980.28203062046736505No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT980.28203062046736505No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT980.28203062046736505No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA940.2705191665707379No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC920.26476343962242427No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC890.25612984919995396No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC890.25612984919995396No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT850.2446183953033268No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG850.2446183953033268No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA820.23598480488085646No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA820.23598480488085646No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA800.23022907793254285No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC790.2273512144583861No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT790.2273512144583861No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.2244733509842293No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT760.21871762403591574No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT740.21296189708760216No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC740.21296189708760216No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC720.2072061701392886No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC720.2072061701392886No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA710.2043283066651318No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC690.19857257971681824No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA660.18993898929434788No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA650.1870611258201911No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT650.1870611258201911No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC650.1870611258201911No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC630.18130539887187752No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT630.18130539887187752No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA620.17842753539772072No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC610.17554967192356394No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC610.17554967192356394No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT610.17554967192356394No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC600.17267180844940716No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT600.17267180844940716No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT570.1640382180269368No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT560.16116035455278002No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT560.16116035455278002No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT550.15828249107862322No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA540.15540462760446644No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.15540462760446644No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA540.15540462760446644No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA520.14964890065615288No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT520.14964890065615288No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT510.14677103718199608No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT510.14677103718199608No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC500.1438931737078393No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA480.13813744675952574No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT480.13813744675952574No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACC480.13813744675952574No Hit
TCGACCGAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT480.13813744675952574No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT480.13813744675952574No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT480.13813744675952574No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA470.13525958328536894No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA460.13238171981121213No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT460.13238171981121213No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCT450.12950385633705536No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA440.12662599286289858No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT430.1237481293887418No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC430.1237481293887418No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT430.1237481293887418No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT420.12087026591458502No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA420.12087026591458502No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC420.12087026591458502No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT420.12087026591458502No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT420.12087026591458502No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT410.11799240244042823No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC410.11799240244042823No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTG410.11799240244042823No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA400.11511453896627143No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT400.11511453896627143No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACC390.11223667549211465No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT390.11223667549211465No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC390.11223667549211465No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT390.11223667549211465No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA380.10935881201795787No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC370.10648094854380108No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA370.10648094854380108No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT370.10648094854380108No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA360.1036030850696443No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA360.1036030850696443No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC360.1036030850696443No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC350.1007252215954875No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACC350.1007252215954875No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA350.1007252215954875No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)