FastQCFastQC Report
Sun 21 May 2023
EGAF00007847825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34748
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA14114.060665362035225No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9102.6188557614826755No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9082.613100034534362No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8752.5181305398871876No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7242.083573155289513No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6621.9051456198917922No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6151.7698860366064235No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6101.7554967192356397No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5991.7238402210199149No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5841.680672268907563No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4981.4331760101300794No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4931.4187866927592954No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4841.3928859214918845No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4791.3784966041211004No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4701.3525958328536896No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4201.20870265914585No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4191.2058247956716934No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4151.1943133417750662No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3951.1367560722919305No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3460.9957407620582479No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3300.9496949464717395No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3270.941061356049269No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3250.9353056291009555No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3100.8921376769886037No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3020.8691147691953495No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3000.8633590422470357No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2970.8547254518245654No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2940.8460918614020951No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2870.8259468170829976No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2860.8230689536088407No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2790.8029239092897433No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2670.7683895475998619No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2400.6906872337976286No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2390.6878093703234719No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2280.6561528721077472No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2160.6216185104178658No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2080.5985956026246115No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2040.5870841487279843No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2030.5842062852538276No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2020.5813284217796708No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2020.5813284217796708No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2010.578450558305514No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2000.5755726948313572No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1940.5583055139864165No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1910.5496719235639461No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1880.5410383331414758No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1850.5324047427190054No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1770.5093818349257511No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1680.4834810636583401No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1680.4834810636583401No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1660.4777253367100265No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1660.4777253367100265No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1640.4719696097617129No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1590.45758029239092896No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1580.4547024289167722No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1570.45182456544261534No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1480.4259237941752043No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1460.42016806722689076No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1460.42016806722689076No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1430.41153447680442035No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1410.40577874985610685No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1370.39426729595947974No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1370.39426729595947974No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1340.3856337055370093No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1340.3856337055370093No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1320.37987797858869576No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1260.36261079774375504No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1240.35685507079544143No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1220.3510993438471279No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1220.3510993438471279No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1210.3482214803729711No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1190.3424657534246575No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1140.3280764360538736No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1140.3280764360538736No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1140.3280764360538736No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1130.32519857257971685No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1130.32519857257971685No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1110.31944284563140324No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1090.3136871186830897No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1080.3108092552089329No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1050.3021756647864625No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1000.2877863474156786No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT980.28203062046736505No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC950.2733970300448947No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC950.2733970300448947No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA940.2705191665707379No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT920.26476343962242427No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA900.2590077126741107No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA900.2590077126741107No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC890.25612984919995396No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC880.25325198572579716No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT860.2474962587774836No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT860.2474962587774836No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT830.23886266835501324No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC830.23886266835501324No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT810.23310694140669966No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.23310694140669966No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA800.23022907793254285No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA790.2273512144583861No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA780.2244733509842293No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.21871762403591574No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG760.21871762403591574No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA750.21583976056175894No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG750.21583976056175894No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC750.21583976056175894No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC720.2072061701392886No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT720.2072061701392886No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA700.201450443190975No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT700.201450443190975No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC700.201450443190975No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC690.19857257971681824No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT690.19857257971681824No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA670.19281685276850466No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA660.18993898929434788No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT650.1870611258201911No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT630.18130539887187752No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT630.18130539887187752No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT620.17842753539772072No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC600.17267180844940716No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA580.1669160815010936No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA580.1669160815010936No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA570.1640382180269368No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT570.1640382180269368No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT540.15540462760446644No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT540.15540462760446644No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCT540.15540462760446644No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT530.15252676413030966No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT520.14964890065615288No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC520.14964890065615288No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA520.14964890065615288No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC510.14677103718199608No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT510.14677103718199608No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC510.14677103718199608No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC510.14677103718199608No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC500.1438931737078393No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.1438931737078393No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC500.1438931737078393No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA480.13813744675952574No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA470.13525958328536894No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT460.13238171981121213No Hit
TCGACCGAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT460.13238171981121213No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA460.13238171981121213No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC450.12950385633705536No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACC450.12950385633705536No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC440.12662599286289858No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT430.1237481293887418No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA420.12087026591458502No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT420.12087026591458502No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT420.12087026591458502No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT410.11799240244042823No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA400.11511453896627143No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT400.11511453896627143No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT400.11511453896627143No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT390.11223667549211465No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA380.10935881201795787No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC370.10648094854380108No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCT370.10648094854380108No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC370.10648094854380108No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT370.10648094854380108No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT370.10648094854380108No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT370.10648094854380108No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT360.1036030850696443No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC360.1036030850696443No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC360.1036030850696443No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT350.1007252215954875No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC350.1007252215954875No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)