FastQCFastQC Report
Sun 21 May 2023
EGAF00007847837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39743
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA17064.292579825378054No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13023.276048612334247No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10362.606748358201444No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10092.5388118662405956No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA9902.4910047052311097No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7321.841833782049669No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7161.801575120146944No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6941.746219460030697No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6701.6858314671766097No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA6651.6732506353320082No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6561.6506051380117255No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6141.5449261505170722No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5791.4568603276048613No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5461.373826837430491No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5431.3662783383237298No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5341.343632841003447No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA5211.310922678207483No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4931.2404700198777143No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4611.1599526960722644No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4491.1297586996452205No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4461.1222102005384598No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4451.1196940341695394No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4221.061822207684372No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3970.9989180484613643No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3570.8982713937045518No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3560.8957552273356314No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3550.8932390609667112No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3400.8554965654329064No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3340.8403995672193845No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3340.8403995672193845No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3320.8353672344815439No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3230.8127217371612612No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3170.7976247389477392No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2830.7120750824044486No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2780.6994942505598469No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2690.6768487532395642No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2660.6693002541328033No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2600.6542032559192814No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2500.6290415922300783No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2480.6240092594922376No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2460.618976926754397No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2410.6063960949097954No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2400.6038799285408751No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2390.6013637621719548No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2360.5938152630651939No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2270.5711697657449111No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2210.5560727675313892No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2200.5535566011624689No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2110.530911103842186No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2090.5258787711043454No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2060.5183302719975844No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2050.5158141056286641No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2020.5082656065219032No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1960.49316860830838133No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1920.4831039428327001No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1900.4780716100948595No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1880.4730392773570189No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1870.4705231109880985No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1830.46045844551241727No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1780.4478776136678157No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1750.44032911456105484No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1730.4352967818232142No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1680.4227159499786126No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1630.410135118134011No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1570.39503811992048915No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1480.37239262260020634No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1450.3648441234934454No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1410.35477945801776417No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1390.34974712527992347No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1380.3472309589110032No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1340.33716629343532195No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1310.32961779432856103No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1280.32206929522180006No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1270.31955312885287973No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1230.3094884633771985No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1220.3069722970082782No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1170.29439146516367665No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1160.29187529879475627No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1160.29187529879475627No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1150.289359132425836No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1110.27929446695015475No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1090.2742621342123141No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1050.26419746873663286No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1040.26168130236771253No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.26168130236771253No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1040.26168130236771253No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1010.2541328032609516No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1010.2541328032609516No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG970.24406813778527037No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT970.24406813778527037No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT920.23148730594066882No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC920.23148730594066882No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT910.22897113957174847No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT890.22393880683390785No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA870.21890647409606723No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG860.21639030772714693No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT860.21639030772714693No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT850.2138741413582266No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT840.2113579749893063No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT830.20884180862038598No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG820.20632564225146569No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT820.20632564225146569No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA750.18871247766902347No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT740.18619631130010317No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC740.18619631130010317No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT720.18116397856226255No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT720.18116397856226255No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT700.1761316458244219No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA690.1736154794555016No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT690.1736154794555016No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT690.1736154794555016No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA660.16606698034874065No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC650.16355081397982035No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT630.1585184812419797No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA630.1585184812419797No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT600.15096998213521878No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA590.14845381576629849No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC590.14845381576629849No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC570.14342148302845784No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC570.14342148302845784No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA570.14342148302845784No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC570.14342148302845784No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT570.14342148302845784No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT570.14342148302845784No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC560.14090531665953754No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA560.14090531665953754No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA560.14090531665953754No Hit
TCGACCGAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT560.14090531665953754No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC550.1383891502906172No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC540.13587298392169692No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT530.1333568175527766No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT530.1333568175527766No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT520.13084065118385627No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG500.12580831844601564No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT500.12580831844601564No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG500.12580831844601564No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC470.11825981933925472No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC450.11322748660141407No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC440.11071132023249376No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA440.11071132023249376No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA430.10819515386357346No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT420.10567898749465315No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA420.10567898749465315No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA420.10567898749465315No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC410.10316282112573284No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT410.10316282112573284No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC410.10316282112573284No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCT410.10316282112573284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)