FastQCFastQC Report
Sun 21 May 2023
EGAF00007847843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42968
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAGCACCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA18004.18916402904487No Hit
ATCAGCACCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA16313.7958480729845463No Hit
ATCAGCACCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13493.1395457084341833No Hit
ATCAGCACCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA12602.932414820331409No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT11882.7648482591696144No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT10042.3366226028672497No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT8732.031744554086762No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT8581.9968348538447218No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT7851.8269409793334574No Hit
ATCAGCACCGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA6531.5197356172035004No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5901.3731148761869298No Hit
ATCAGCACCGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA5721.331223235896481No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT5401.256749208713461No Hit
ATCAGCACCGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA5171.2032210016756657No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT5061.1776205548315024No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4921.1450381679389312No Hit
ATCAGCACCGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA4891.1380562278905233No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4541.0566002606590952No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4531.0542729473096257No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4230.9844535468255446No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3930.9146341463414633No Hit
ATCAGCACCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3810.886706386147831No Hit
ATCAGCACCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3740.8704151927015452No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3680.8564513126047292No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3680.8564513126047292No Hit
ATCAGCACCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3620.8424874325079129No Hit
ATCAGCACCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3600.837832805808974No Hit
ATCAGCACCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3570.830850865760566No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3420.7959411655185255No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT3370.7843045987711785No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3310.7703407186743623No Hit
ATCAGCACCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3220.749394898529138No Hit
ATCAGCACCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3210.7470675851796685No Hit
ATCAGCACCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3210.7470675851796685No Hit
ATCAGCACCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3190.7424129584807299No Hit
ATCAGCACCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3130.7284490783839136No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3020.7028486315397505No Hit
ATCAGCACCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3000.6981940048408117No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3000.6981940048408117No Hit
ATCAGCACCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2860.6656116179482405No Hit
ATCAGCACCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2850.6632843045987712No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2820.6563023645503631No Hit
ATCAGCACCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2730.6353565444051387No Hit
ATCAGCACCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2670.6213926643083224No Hit
ATCAGCACCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2590.6027741575125675No Hit
ATCAGCACCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2520.5864829640662819No Hit
ATCAGCACCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2510.5841556507168124No Hit
ATCAGCACCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2490.5795010240178737No Hit
ATCAGCACCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2480.5771737106684044No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2400.5585552038726493No Hit
ATCAGCACCGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2390.55622789052318No Hit
ATCAGCACCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2380.5539005771737107No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2290.5329547570284863No Hit
ATCAGCACCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2150.5003723701359151No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT2100.4887358033885682No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2010.4677899832433439No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1980.4608080431949358No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1920.44684416309811953No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1900.44218953639918074No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1850.43055296965183393No Hit
ATCAGCACCGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1830.42589834295289514No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1740.40495252280767086No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1720.40029789610873207No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1710.3979705827592627No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1710.3979705827592627No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1660.38633401601191586No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1570.36538819586669147No Hit
ATCAGCACCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1490.34676968907093647No Hit
ATCAGCACCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1390.3234965555762428No Hit
ATCAGCACCGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1370.318841928877304No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1300.3025507354310184No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1290.30022342208154906No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1270.29556879538261027No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1250.29091416868367154No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1210.28160491528579407No Hit
ATCAGCACCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1190.27695028858685533No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1170.2722956618879166No Hit
ATCAGCACCGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1160.2699683485384472No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1130.26298640849003907No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1110.25833178179110033No Hit
ATCAGCACCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1070.2490225283932229No Hit
ATCAGCACCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1040.24204058834481476No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1030.23971327499534537No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1000.23273133494693723No Hit
ATCAGCACCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG990.2304040215974679No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT980.2280767082479985No Hit
ATCAGCACCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA970.22574939489852916No Hit
ATCAGCACCGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA930.21644014150065163No Hit
ATCAGCACCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA900.2094582014522435No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT900.2094582014522435No Hit
ATCAGCACCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG890.20713088810277416No Hit
ATCAGCACCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA870.20247626140383543No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT860.20014894805436603No Hit
ATCAGCACCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA850.19782163470489664No Hit
ATCAGCACCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG840.1954943213554273No Hit
TCGACCGAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT840.1954943213554273No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC840.1954943213554273No Hit
ATCAGCACCGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA780.18153044125861106No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT760.1768758145596723No Hit
ATCAGCACCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA750.17454850121020293No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT750.17454850121020293No Hit
ATCAGCACCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA730.1698938745112642No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT730.1698938745112642No Hit
ATCAGCACCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA730.1698938745112642No Hit
ATCAGCACCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA700.16291193446285607No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT690.1605846211133867No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT690.1605846211133867No Hit
ATCAGCACCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA680.15825730776391733No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT680.15825730776391733No Hit
ATCAGCACCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA640.14894805436603983No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT620.1442934276671011No Hit
ATCAGCACCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA610.14196611431763173No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT600.13963880096816234No Hit
ATCAGCACCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA590.13731148761869297No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC590.13731148761869297No Hit
ATCAGCACCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA580.1349841742692236No Hit
ATCAGCACCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA580.1349841742692236No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT580.1349841742692236No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT580.1349841742692236No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT570.13265686091975423No Hit
ATCAGCACCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA570.13265686091975423No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACT550.1280022342208155No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT550.1280022342208155No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT550.1280022342208155No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC550.1280022342208155No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.12334760752187675No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT530.12334760752187675No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT520.12102029417240738No Hit
ATCAGCACCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA520.12102029417240738No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT520.12102029417240738No Hit
ATCAGCACCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA520.12102029417240738No Hit
ATCAGCACCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA500.11636566747346862No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT490.11403835412399925No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT490.11403835412399925No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT480.11171104077452988No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT480.11171104077452988No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCT470.10938372742506051No Hit
ATCAGCACCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA470.10938372742506051No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT470.10938372742506051No Hit
ATCAGCACCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA460.10705641407559115No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT460.10705641407559115No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT460.10705641407559115No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT450.10472910072612175No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCT450.10472910072612175No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC440.10240178737665238No Hit
ATCAGCACCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA430.10007447402718302No Hit
ATCAGCACCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA430.10007447402718302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)