FastQCFastQC Report
Sun 21 May 2023
EGAF00007847857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38710
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA15293.949883750968742No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA14713.800051666236115No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10332.668561095324206No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10102.609144923792302No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT8022.071816068199432No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7251.8729010591578406No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6951.795401704985792No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6691.7282355980366828No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6651.7179023508137432No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6611.7075691035908034No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5731.4802376646861275No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5391.3924050632911391No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5341.3794885042624645No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4851.2529062257814518No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4781.2348230431413072No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4641.198656677861018No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4421.1418238181348488No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4191.0824076466029449No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3981.028158098682511No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3710.9584086799276673No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3590.927408938258848No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3590.927408938258848No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3540.914492379230173No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3470.8964091965900284No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3440.8886592611728237No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3400.8783260139498837No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3190.8240764660294497No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3150.81374321880651No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3040.7853267889434254No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2990.7724102299147507No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2940.7594936708860759No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2820.7284939292172565No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2640.6819943167140274No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2580.6664944458796176No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2560.6613278222681478No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2300.5941617153190389No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2300.5941617153190389No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2150.5554120382330148No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2120.5476621028158098No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2100.5424954792043399No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2070.5347455437871351No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2030.5244122965641953No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1930.4985791785068458No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1830.4727460604494963No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1830.4727460604494963No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1820.47016274864376134No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1810.46757943683802633No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1810.46757943683802633No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1780.4598295014208215No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1770.4572461896150865No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1770.4572461896150865No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1740.4494962541978817No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1730.4469129423921467No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1640.4236631361405322No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1640.4236631361405322No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1600.41332988891759237No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1580.40816326530612246No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1570.4055799535003875No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1570.4055799535003875No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1550.40041332988891754No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1510.39008008266597777No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1440.37199690002583313No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1420.3668302764143632No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1410.36424696460862827No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1410.36424696460862827No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1390.35908034099715835No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1340.34616378196848363No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1290.3332472229398088No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1270.32808059932833894No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1250.32291397571686903No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1230.3177473521053991No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1210.3125807284939292No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1210.3125807284939292No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1210.3125807284939292No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1150.29708085765951947No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1150.29708085765951947No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1150.29708085765951947No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1140.29449754585378457No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1130.29191423404804956No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1110.2867476104365797No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1030.26608111599070006No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1030.26608111599070006No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1020.26349780418496516No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1000.25833118057349524No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC960.24799793335055542No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT960.24799793335055542No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT940.2428313097390855No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT920.2376646861276156No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT910.23508137432188067No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA900.2324980625161457No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA900.2324980625161457No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA890.22991475071041076No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT880.2273314389046758No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT870.22474812709894085No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT870.22474812709894085No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT870.22474812709894085No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC820.2118315680702661No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG800.20666494445879618No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT800.20666494445879618No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA770.19891500904159135No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG770.19891500904159135No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA760.19633169723585636No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC760.19633169723585636No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT760.19633169723585636No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT740.19116507362438648No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT700.18083182640144665No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT700.18083182640144665No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT690.1782485145957117No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA680.17566520278997677No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA680.17566520278997677No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA660.17049857917850686No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT660.17049857917850686No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT640.16533195556703695No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC630.162748643761302No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC620.16016533195556704No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC620.16016533195556704No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC620.16016533195556704No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA620.16016533195556704No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC620.16016533195556704No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG610.15758202014983208No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT580.14983208473262724No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC580.14983208473262724No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA580.14983208473262724No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA570.14724877292689229No Hit
TCGACCGAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT570.14724877292689229No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.14466546112115733No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC550.14208214931542237No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC550.14208214931542237No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA550.14208214931542237No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC530.13691552570395246No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAACACGTAGGGTGCGAGCGTTA530.13691552570395246No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC520.13433221389821753No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT520.13433221389821753No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA520.13433221389821753No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAACACGTAGGGTGCGAGCGTTA510.13174890209248258No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT500.12916559028674762No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAACACGTAGGGTGCGAGCGTTA490.12658227848101267No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT490.12658227848101267No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC480.12399896667527771No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT480.12399896667527771No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT480.12399896667527771No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT480.12399896667527771No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACT470.12141565486954276No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT470.12141565486954276No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT470.12141565486954276No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT470.12141565486954276No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC470.12141565486954276No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA460.1188323430638078No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC460.1188323430638078No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCT450.11624903125807284No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG450.11624903125807284No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA430.11108240764660296No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT430.11108240764660296No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA430.11108240764660296No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA430.11108240764660296No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC430.11108240764660296No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA430.11108240764660296No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA420.108499095840868No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT420.108499095840868No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAACACGTAGGGTGCGAGCGTTA420.108499095840868No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT410.10591578403513305No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGATACCCACGCT400.10333247222939809No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA390.10074916042366314No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC390.10074916042366314No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA390.10074916042366314No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)