FastQCFastQC Report
Sun 21 May 2023
EGAF00007847867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32248
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13434.164599354998759No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA13194.090176134954106No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8372.595509799057306No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8202.542793351525676No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6972.1613743487968247No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6241.935003721161002No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5841.810965021086579No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5801.7985611510791366No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5501.7055321260233194No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5111.5845943934507567No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5111.5845943934507567No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5091.5783924584470355No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4671.448151823368891No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4511.3985363433391218No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4391.3613247333167948No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4141.2838005457702804No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4141.2838005457702804No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4001.2403870007442321No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3931.2186802282312081No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3861.1969734557181841No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3831.1876705532126024No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3801.1783676507070204No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3371.0450260481270157No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3361.0419250806251552No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3180.9861076655916645No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3050.945795088067477No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3050.945795088067477No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2840.8806747705284048No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2800.8682709005209625No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2780.8620689655172413No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2750.8527660630116597No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2700.8372612255023568No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2620.8124534854874721No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2520.7814438104688662No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2500.7752418754651451No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2490.7721409079632846No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2400.7442322004465393No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2280.7070205904242124No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2240.69461672041677No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2170.6729099479037459No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2160.6698089804018854No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2150.6667080129000248No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2030.6294964028776978No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2020.6263954353758373No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1930.5984867278590921No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1890.5860828578516497No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1810.5612751178367651No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1790.5550731828330439No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1760.5457702803274621No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1700.5271644753162986No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1690.5240635078144381No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1570.4868518977921112No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1490.4620441577772265No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1420.4403373852642024No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1370.4248325477548995No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1350.4186306127511784No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1350.4186306127511784No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1320.40932771024559667No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1320.40932771024559667No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1290.40002480774001486No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1270.39382287273629374No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1260.3907219052344331No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1190.36901513272140907No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1150.35661126271396676No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1140.3535102952121062No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1040.3225006201935004No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1030.3193996526916398No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1020.3162986851897792No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1010.31319771768791865No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1010.31319771768791865No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT990.30699578268419747No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA970.3007938476804763No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA960.2976928801786157No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT950.2945919126767551No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA940.2914909451748946No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA910.28218804266931286No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA910.28218804266931286No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT900.27908707516745224No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT890.2759861076655917No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA860.26668320516000993No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA840.2604812701562888No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG830.2573803026544282No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC820.2542793351525676No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC780.24187546514512526No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT780.24187546514512526No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA750.23257256263954354No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT730.22637062763582239No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT710.2201686926321012No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT710.2201686926321012No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT660.20466385512279833No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA660.20466385512279833No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG650.20156288762093774No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT650.20156288762093774No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA630.19536095261721656No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC620.192259985115356No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC620.192259985115356No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA610.1891590176134954No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA600.18605805011163482No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA590.18295708260977425No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA560.1736541801041925No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA560.1736541801041925No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA550.17055321260233192No Hit
TCGACCGAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT550.17055321260233192No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA550.17055321260233192No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA540.16745224510047135No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC520.1612503100967502No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT520.1612503100967502No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT510.1581493425948896No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.15504837509302902No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA500.15504837509302902No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT500.15504837509302902No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT480.14884644008930786No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA480.14884644008930786No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA470.1457454725874473No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG460.1426445050855867No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT430.13334160258000496No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA420.1302406350781444No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT410.1271396675762838No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA410.1271396675762838No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA400.12403870007442323No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT390.12093773257256263No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC380.11783676507070207No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC370.11473579756884147No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA370.11473579756884147No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA370.11473579756884147No Hit
TCGACCGAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT370.11473579756884147No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG360.1116348300669809No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT350.1085338625651203No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA350.1085338625651203No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT340.10543289506325974No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA340.10543289506325974No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT340.10543289506325974No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC330.10233192756139917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)