FastQCFastQC Report
Sun 21 May 2023
EGAF00007847879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37524
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13153.504423835412003No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12843.421810041573393No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10562.8141989126958746No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10532.8062040294211705No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6881.8334932309988272No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6201.6522758767721992No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6071.6176313825818145No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4931.313825818143055No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4921.311160857051487No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4901.305830934868351No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4901.305830934868351No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4861.2951710905020788No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4771.2711864406779663No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4601.225882102121309No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4541.2098923355719007No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4511.2018974522971964No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4191.1166186973670185No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3961.0553245922609529No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3891.0366698646199766No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3861.0286749813452722No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3781.0073552926127278No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3750.9993604093380237No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3750.9993604093380237No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3550.9460611875066625No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3500.932736382048822No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3250.8661123547596205No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3250.8661123547596205No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3190.8501225882102121No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3070.8181430551113953No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3040.8101481718366912No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3020.804818249653555No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3010.8021532885619871No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2890.7701737554631702No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2890.7701737554631702No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2860.762178872188466No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2830.7541839889137618No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2800.7461891056390577No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2700.7195394947233771No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2650.7062146892655368No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2470.6582453896173116No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2470.6582453896173116No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2360.628930817610063No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2290.6102760899690864No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2210.5889564012365419No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2190.5836264790534058No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2160.5756315957787016No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2110.5623067903208613No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2080.5543119070461572No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2050.546317023771453No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2030.5409871015883169No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2020.5383221404967488No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1990.5303272572220445No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1950.5196674128557722No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1940.5170024517642042No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1910.5090075684895No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1910.5090075684895No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1810.4823579575738194No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1760.4690331521159791No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1730.4610382688412749No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1720.4583733077497068No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1710.4557083466581388No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1650.4397185801087304No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1560.4157339302846178No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1510.4024091248267775No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1480.3944142415520734No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1430.381089436094233No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1400.37309455281952886No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1390.37042959172796075No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1380.3677646306363927No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1290.3437799808122801No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1240.3304551753544398No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1240.3304551753544398No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1230.32779021426287175No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1220.3251252531713037No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1210.32246029207973564No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1190.31713036989659954No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1170.3118004477134634No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1160.30913548662189533No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1150.3064705255303273No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1140.30380556443875917No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1110.295810681164055No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1090.29048075898091885No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1090.29048075898091885No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1080.2878157978893508No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1050.27982091461464664No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1010.2691610702483744No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1010.2691610702483744No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1010.2691610702483744No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1010.2691610702483744No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA950.253171303698966No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA940.2505063426073979No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT940.2505063426073979No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC930.24784138151582985No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT900.2398464982411257No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC900.2398464982411257No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT900.2398464982411257No Hit
TCGACCGAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT890.23718153714955761No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA880.23451657605798956No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT860.2291866538748534No Hit
TCGACCGAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT860.2291866538748534No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA860.2291866538748534No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC830.22119177060014925No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC800.21319688732544506No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT800.21319688732544506No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA770.20520200405074085No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA760.2025370429591728No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT760.2025370429591728No Hit
TCGACCGAGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT750.19987208186760475No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA750.19987208186760475No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC740.1972071207760367No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA720.19187719859290053No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT720.19187719859290053No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA720.19187719859290053No Hit
TCGACCGAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT710.18921223750133248No Hit
TCGACCGAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT680.1812173542266283No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA680.1812173542266283No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC680.1812173542266283No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA670.17855239313506024No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGGGCAAGCGTTA670.17855239313506024No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT660.17588743204349216No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA640.17055750986035603No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA610.16256262658565185No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA600.1598976654940838No Hit
TCGACCGAGCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT580.15456774331094766No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC580.15456774331094766No Hit
TCGACCGAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT570.15190278221937958No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGGGGCAAGCGTTA570.15190278221937958No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA570.15190278221937958No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.14923782112781153No Hit
TCGACCGAGCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC540.1439078989446754No Hit
TCGACCGAGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT540.1439078989446754No Hit
TCGACCGAGCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.14124293785310735No Hit
TCGACCGAGCCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC510.13591301566997122No Hit
TCGACCGAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT510.13591301566997122No Hit
TCGACCGAGCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT500.13324805457840316No Hit
TCGACCGAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT490.13058309348683508No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGGGGCAAGCGTTA490.13058309348683508No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA490.13058309348683508No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA490.13058309348683508No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC480.12791813239526703No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA480.12791813239526703No Hit
TCGACCGAGCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC470.12525317130369895No Hit
TCGACCGAGCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT470.12525317130369895No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA460.1225882102121309No Hit
TCGACCGAGCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT450.11992324912056285No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT450.11992324912056285No Hit
TCGACCGAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT440.11725828802899478No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA440.11725828802899478No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA430.1145933269374267No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA430.1145933269374267No Hit
TCGACCGAGCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT430.1145933269374267No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC420.11192836584585865No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA420.11192836584585865No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA420.11192836584585865No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA420.11192836584585865No Hit
TCGACCGAGCCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCT410.1092634047542906No Hit
TCGACCGAGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT410.1092634047542906No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA400.10659844366272253No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGGGCAAGCGTTA400.10659844366272253No Hit
TCGACCGAGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC400.10659844366272253No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA390.10393348257115445No Hit
TCGACCGAGCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT390.10393348257115445No Hit
TCGACCGAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT390.10393348257115445No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA390.10393348257115445No Hit
TCGACCGAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT380.1012685214795864No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA380.1012685214795864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)