FastQCFastQC Report
Sun 21 May 2023
EGAF00007847887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51920
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA23744.572419106317412No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA21914.219953775038521No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG12612.4287365177195688No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11522.2187981510015407No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG11202.157164869029276No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10402.0030816640986133No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT9211.7738828967642526No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7281.4021571648690292No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6681.2865947611710324No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6531.257704160246533No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6401.2326656394453006No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT6321.2172573189522342No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6321.2172573189522342No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5881.1325115562403698No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5661.0901386748844377No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5591.0766563944530045No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5541.0670261941448382No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5501.059322033898305No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5461.0516178736517718No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA5441.0477657935285054No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5281.0169491525423728No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA5040.970724191063174No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5020.9668721109399074No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT5000.9630200308166409No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4830.9302773497688752No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT4590.8840523882896764No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4420.8513097072419106No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4390.8455315870570107No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT4240.8166409861325115No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4050.7800462249614791No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3990.7684899845916795No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3920.7550077041602465No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3890.7492295839753467No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3700.7126348228043143No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3600.6933744221879815No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3590.6914483821263483No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3510.676040061633282No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3450.6644838212634823No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3290.6336671802773497No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT3210.6182588597842835No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3120.6009244992295839No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3090.5951463790446841No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3060.5893682588597843No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2930.5643297380585517No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2860.5508474576271186No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2830.5450693374422189No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2780.5354391371340524No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2700.5200308166409862No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2660.5123266563944531No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2560.4930662557781202No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2550.4911402157164869No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2520.485362095531587No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2500.48151001540832045No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2470.47573189522342063No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2440.4699537750385208No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2330.4487673343605547No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2280.4391371340523883No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2270.43721109399075503No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2260.4352850539291217No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2180.41987673343605547No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2160.41602465331278893No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2150.41409861325115566No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2110.40639445300462246No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT2080.40061633281972264No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2040.39291217257318956No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1900.3659476117103236No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1880.362095531587057No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1880.362095531587057No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1820.3505392912172573No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1810.348613251155624No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1800.34668721109399075No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1770.3409090909090909No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1740.33513097072419107No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1730.3332049306625578No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1710.3293528505392912No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1680.3235747303543914No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1660.31972265023112484No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1620.3120184899845917No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1610.3100924499229584No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1600.30816640986132515No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1520.29275808936825887No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1520.29275808936825887No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1510.29083204930662554No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1490.286979969183359No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1370.2638674884437596No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1360.26194144838212635No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1350.2600154083204931No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1340.2580893682588598No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1320.2542372881355932No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1270.24460708782742682No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1270.24460708782742682No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1270.24460708782742682No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1260.2426810477657935No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1220.2349768875192604No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1210.23305084745762714No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1200.23112480739599386No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1190.22919876733436054No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1160.22342064714946072No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.2022342064714946No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.20030816640986132No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1040.20030816640986132No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC980.18875192604006163No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT980.18875192604006163No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT960.18489984591679506No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT960.18489984591679506No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT930.17912172573189522No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA910.17526964560862865No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT910.17526964560862865No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT900.17334360554699538No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT880.1694915254237288No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT870.16756548536209553No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT860.16563944530046226No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC840.1617873651771957No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA840.1617873651771957No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA840.1617873651771957No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA830.15986132511556242No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT830.15986132511556242No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC830.15986132511556242No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT830.15986132511556242No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA810.15600924499229585No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT800.15408320493066258No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA790.15215716486902928No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT780.15023112480739598No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA760.14637904468412943No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA760.14637904468412943No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA750.14445300462249613No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT740.14252696456086286No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA740.14252696456086286No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT740.14252696456086286No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA730.1406009244992296No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA730.1406009244992296No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA700.13482280431432975No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC700.13482280431432975No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA690.13289676425269645No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA690.13289676425269645No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT690.13289676425269645No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT690.13289676425269645No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA680.13097072419106318No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT680.13097072419106318No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC670.1290446841294299No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA670.1290446841294299No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT670.1290446841294299No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCT660.1271186440677966No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT650.12519260400616333No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT640.12326656394453005No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA640.12326656394453005No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT620.11941448382126348No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG620.11941448382126348No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT620.11941448382126348No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT610.1174884437596302No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA610.1174884437596302No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC610.1174884437596302No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCT600.11556240369799693No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC590.11363636363636363No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT590.11363636363636363No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT580.11171032357473036No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA570.10978428351309708No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA570.10978428351309708No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA560.10785824345146379No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC560.10785824345146379No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT550.1059322033898305No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC540.10400616332819723No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCT540.10400616332819723No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG530.10208012326656393No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCT530.10208012326656393No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)