FastQCFastQC Report
Sun 21 May 2023
EGAF00007847936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50360
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13112.6032565528196985No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12242.430500397140588No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9411.868546465448769No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9391.8645750595710882No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7671.5230341540905479No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA7621.5131056393963462No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA7311.4515488482922956No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6801.3502779984114377No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6731.336378077839555No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6481.2867355043685464No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6431.2768069896743446No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6341.2589356632247817No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT6301.2509928514694202No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6301.2509928514694202No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6201.2311358220810167No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5911.1735504368546466No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5741.1397934868943607No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA5691.1298649722001588No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5511.0941223193010325No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5341.0603653693407467No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA5020.9968228752978554No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4950.982922954725973No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4760.9451945988880063No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4760.9451945988880063No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4670.9273232724384431No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT4500.8935663224781574No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4310.8558379666401905No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4290.85186656076251No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4190.8320095313741064No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4030.8002382843526609No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3880.7704527402700556No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3770.7486100079428117No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3760.7466243050039714No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA3720.73868149324861No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3670.7287529785544083No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3610.7168387609213661No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3400.6751389992057188No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3400.6751389992057188No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3350.665210484511517No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA3330.6612390786338364No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT3110.6175536139793487No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3080.6115965051628276No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3060.607625099285147No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3060.607625099285147No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3050.6056393963463066No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2980.5917394757744242No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2930.5818109610802225No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2880.5718824463860206No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2830.561953931691819No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2830.561953931691819No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2830.561953931691819No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA2770.5500397140587767No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2740.5440826052422558No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2630.522239872915012No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2580.5123113582208102No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2550.5063542494042892No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA2550.5063542494042892No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2490.494440031771247No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2440.4845115170770453No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2440.4845115170770453No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2380.47259729944400314No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2370.4706115965051628No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2320.46068308181096107No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT2270.4507545671167594No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2260.448768864177919No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2130.42295472597299444No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2080.41302621127879263No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2070.41104050833995237No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2060.40905480540111194No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT2000.3971405877680699No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1940.3852263701350278No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1880.3733121525019857No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1850.3673550436854646No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1830.36338363780778393No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1800.3574265289912629No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1660.32962668784749805No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1640.3256552819698173No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1590.31572676727561555No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1550.30778395552025417No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1540.3057982525814138No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1520.3018268467037331No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1490.2958697378872121No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1470.29189833200953136No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1470.29189833200953136No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1460.289912629070691No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1420.2819698173153296No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1400.2779984114376489No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1380.27402700555996823No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1380.27402700555996823No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1340.26608419380460685No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1320.2621127879269261No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1300.2581413820492454No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1250.2482128673550437No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1250.2482128673550437No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1170.23232724384432088No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1160.23034154090548054No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1130.2243844320889595No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1100.21842732327243844No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1090.2164416203335981No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1060.21048451151707706No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1000.19857029388403494No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT980.19459888800635425No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA970.1926131850675139No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT970.1926131850675139No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC970.1926131850675139No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC960.19062748212867356No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC930.1846703733121525No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT920.18268467037331215No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA910.1806989674344718No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC910.1806989674344718No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA880.17474185861795077No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA870.1727561556791104No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA870.1727561556791104No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT860.17077045274027006No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA860.17077045274027006No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA860.17077045274027006No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC830.16481334392374902No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC820.16282764098490865No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.1608419380460683No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.1608419380460683No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCCGGCT800.15885623510722796No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT790.15687053216838762No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.15488482922954724No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT770.1528991262907069No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCCGGCT730.1449563145353455No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA720.14297061159650515No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT710.1409849086576648No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA710.1409849086576648No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT710.1409849086576648No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT700.13899920571882446No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC690.13701350277998411No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCCGGCT630.12509928514694202No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT630.12509928514694202No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.12311358220810167No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT620.12311358220810167No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT610.12112787926926133No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC600.11914217633042098No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG590.11715647339158061No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT590.11715647339158061No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC580.11517077045274027No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA580.11517077045274027No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC580.11517077045274027No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGGGGCGAGCGTTG570.11318506751389992No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT570.11318506751389992No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT570.11318506751389992No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA570.11318506751389992No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA570.11318506751389992No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT570.11318506751389992No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT570.11318506751389992No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT560.11119936457505958No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCCGGCT550.10921366163621922No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG550.10921366163621922No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT550.10921366163621922No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA540.10722795869737888No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT540.10722795869737888No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA530.10524225575853853No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT530.10524225575853853No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.10524225575853853No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCCGGCT520.10325655281969816No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA520.10325655281969816No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC520.10325655281969816No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCCGGCT510.10127084988085781No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)