FastQCFastQC Report
Sun 21 May 2023
EGAF00007847937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50360
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12402.4622716441620334No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11162.21604447974583No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9451.8764892772041304No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT9061.7990468625893565No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7891.5667196187450358No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7861.5607625099285147No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7631.5150913423351866No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT7631.5150913423351866No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA7551.4992057188244639No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA7211.4316918189038919No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6921.3741064336775217No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT6571.3046068308181096No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6511.2926926131850673No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6151.221207307386815No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5651.1219221604447973No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5491.0901509134233518No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA5171.0266084193804605No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5061.004765687053217No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4910.9749801429706116No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4840.9610802223987293No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4800.9531374106433679No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4740.9412231930103256No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4720.9372517871326449No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4680.9293089753772835No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4670.9273232724384431No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4600.9134233518665608No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA4350.8637807783955521No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4330.8598093725178714No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT4100.8141382049245434No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4060.806195393169182No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT4060.806195393169182No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4060.806195393169182No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT4040.8022239872915011No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4000.7942811755361397No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3800.7545671167593329No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3710.7366957903097697No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3680.7307386814932486No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA3670.7287529785544083No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3640.7227958697378872No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3550.7049245432883241No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3450.6850675138999206No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA3360.6671961874503575No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3360.6671961874503575No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3340.6632247815726767No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3260.647339158061954No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3240.6433677521842732No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT3150.6254964257347101No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2910.5778395552025417No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2800.5559968228752978No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2710.5381254964257347No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2700.5361397934868944No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2580.5123113582208102No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA2570.5103256552819698No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2540.5043685464654488No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2470.4904686258935663No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2460.488482922954726No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2450.4864972200158856No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2420.48054011119936463No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2400.47656870532168394No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2400.47656870532168394No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2360.46862589356632245No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA2300.4567116759332804No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2250.4467831612390787No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2240.4447974583002383No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2220.44082605242255757No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA2130.42295472597299444No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2110.41898332009531375No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2110.41898332009531375No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2020.40111199364575056No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA2000.3971405877680699No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1980.3931691818903892No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1960.3891977760127085No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1950.3872120730738682No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1920.3812549642573471No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1910.37926926131850675No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1880.3733121525019857No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1860.369340746624305No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1860.369340746624305No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1750.3474980142970611No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1740.3455123113582208No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1660.32962668784749805No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1620.3216838760921366No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1610.31969817315329624No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1600.3177124702144559No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1420.2819698173153296No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1410.2799841143764893No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1360.27005559968228754No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1360.27005559968228754No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1360.27005559968228754No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1320.2621127879269261No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1320.2621127879269261No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1310.2601270849880858No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1310.2601270849880858No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1310.2601270849880858No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1300.2581413820492454No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1300.2581413820492454No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1260.25019857029388404No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1170.23232724384432088No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1160.23034154090548054No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1120.22239872915011916No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1070.2124702144559174No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1060.21048451151707706No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1060.21048451151707706No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC950.18864177918983321No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG930.1846703733121525No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA910.1806989674344718No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC910.1806989674344718No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT900.17871326449563146No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA900.17871326449563146No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCCGGCT870.1727561556791104No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT870.1727561556791104No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC860.17077045274027006No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT830.16481334392374902No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA780.15488482922954724No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC760.15091342335186655No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG740.14694201747418587No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT740.14694201747418587No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA730.1449563145353455No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT720.14297061159650515No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA720.14297061159650515No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA710.1409849086576648No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC710.1409849086576648No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT680.13502779984114377No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT680.13502779984114377No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA680.13502779984114377No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA670.13304209690230342No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT670.13304209690230342No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC660.13105639396346305No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT650.1290706910246227No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC640.12708498808578236No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA630.12509928514694202No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT630.12509928514694202No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT630.12509928514694202No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCCGGCT620.12311358220810167No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT620.12311358220810167No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA620.12311358220810167No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCCGGCT610.12112787926926133No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG610.12112787926926133No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA590.11715647339158061No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA590.11715647339158061No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.11715647339158061No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC580.11517077045274027No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT580.11517077045274027No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCCGGCT580.11517077045274027No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT570.11318506751389992No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT570.11318506751389992No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC570.11318506751389992No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA570.11318506751389992No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT570.11318506751389992No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA570.11318506751389992No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.11119936457505958No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT560.11119936457505958No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCCGGCT550.10921366163621922No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT550.10921366163621922No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC550.10921366163621922No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA530.10524225575853853No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCCGGCT510.10127084988085781No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT510.10127084988085781No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)