FastQCFastQC Report
Sun 21 May 2023
EGAF00007847940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56051
Sequences flagged as poor quality0
Sequence length250
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13022.322884515887317No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12612.2497368468002357No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA10771.9214643806533336No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA10711.9107598437137607No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9701.7305668052309504No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9521.6984531944122316No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT7731.379101175714974No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA7691.3719648177552586No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA7581.3523398333660417No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7271.2970330591782484No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7251.2934648801983908No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7041.2559990009098858No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6791.2113967636616654No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6761.206044495191879No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6371.136465005084655No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6201.1061354837558652No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6111.090078678346506No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT6061.081158230896862No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6001.0704536939572888No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5801.0347719041587127No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5611.000874203850065No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5580.9955219353802787No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5570.9937378458903499No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA5500.9812492194608481No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA5420.9669765035414177No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA5300.9455674296622718No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4990.8902606554744785No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4920.877772029044977No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4740.8456584182262583No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4710.8403061497564719No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4550.8117607179176107No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4500.8028402704679668No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4040.7207721539312412No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3950.7047153485218819No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3870.6904426326024514No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3840.6850903641326649No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3690.6583290217837326No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3660.6529767533139462No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3580.6387040373945158No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3540.6315676794348004No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3480.6208631424952276No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3480.6208631424952276No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3460.6172949635153699No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3440.6137267845355123No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3190.5691245472872919No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3140.5602040998376479No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3130.558420010347719No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3110.5548518313678614No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2940.5245223100390716No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2870.5120336836095698No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2840.5066814151397834No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2820.5031132361599258No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2800.49954505718006814No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2730.4870564307505664No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2700.48170416228078006No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2660.47456780432106477No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2580.46029508840163424No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2530.45137464095199015No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2530.45137464095199015No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2490.44423828299227486No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2340.4174769406433427No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2310.4121246721735562No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2300.4103405826836274No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2230.3978519562541257No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2170.3871474193145528No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA2100.3746587928850511No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA2080.3710906139051935No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1920.34254518206633244No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1880.3354088241066172No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1860.3318406451267596No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1810.3229201976771155No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1760.31399975022747145No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1580.28188613940875273No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1580.28188613940875273No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1540.27474978144903744No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1540.27474978144903744No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1520.2711816024691799No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1510.269397512979251No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1480.2640452445094646No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1440.2569088865497493No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1410.2515566180799629No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1410.2515566180799629No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1390.24798843910010526No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1390.24798843910010526No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT1370.24442026012024762No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1370.24442026012024762No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1360.24263617063031878No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1360.24263617063031878No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1360.24263617063031878No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1340.2390679916504612No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1340.2390679916504612No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1310.23371572318067474No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1310.23371572318067474No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1300.23193163369074596No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1300.23193163369074596No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1280.22836345471088831No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1250.22301118624110183No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1250.22301118624110183No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1250.22301118624110183No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1240.22122709675117305No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1190.21230664930152895No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT1180.21052255981160017No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG1150.2051702913418137No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1140.20338620185188488No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1070.1908975754223832No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG1070.1908975754223832No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1060.18911348593245436No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1040.18554530695259674No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1020.18197712797273913No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1010.1801930384828103No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA990.17662485950295267No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT980.17484077001302384No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA980.17484077001302384No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT980.17484077001302384No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC970.17305668052309503No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC960.17127259103316622No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA950.1694885015432374No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA920.16413623307345096No Hit
TACACATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT910.16235214358352215No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT890.1587839646036645No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT890.1587839646036645No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA890.1587839646036645No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA890.1587839646036645No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT870.1552157856238069No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT850.15164760664394927No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT840.14986351715402044No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA840.14986351715402044No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA830.14807942766409163No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC830.14807942766409163No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA830.14807942766409163No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT820.14629533817416282No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA810.144511248684234No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCC790.14094306970437637No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT780.13915898021444756No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC770.13737489072451872No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC770.13737489072451872No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC750.1338067117446611No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT740.1320226222547323No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC740.1320226222547323No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.1302385327648035No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA720.12845444327487465No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT700.12488626429501704No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT690.12310217480508823No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT680.12131808531515939No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT670.1195339958252306No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA660.11774990633530177No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAACACGTAAGGTGCGAGCGTTG660.11774990633530177No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT650.11596581684537298No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA650.11596581684537298No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT640.11418172735544416No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCACGCT630.11239763786551533No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG630.11239763786551533No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT630.11239763786551533No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC630.11239763786551533No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA630.11239763786551533No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA620.11061354837558653No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT620.11061354837558653No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC610.1088294588856577No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA610.1088294588856577No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC610.1088294588856577No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.10704536939572888No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG600.10704536939572888No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC600.10704536939572888No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.10704536939572888No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA600.10704536939572888No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA600.10704536939572888No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA590.10526127990580009No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC590.10526127990580009No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA570.10169310092594244No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA570.10169310092594244No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)