FastQCFastQC Report
Sun 21 May 2023
EGAF00007847943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49470
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA21774.400646856680817No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA20024.046897109359207No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG17253.4869617950272893No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG15823.1978977157873456No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG14562.943197897715787No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG13472.722862340812614No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6911.396806145138468No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6881.3907418637558115No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6711.3563776025874268No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6551.3240347685465939No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6381.2896705073782089No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6111.2350919749343037No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5971.206791995148575No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5791.170406306852638No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5601.131999191429149No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5321.0753992318576915No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5241.0592278148372751No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5081.0268849807964422No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5051.020820699413786No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4991.0086921366484738No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4680.9460278956943602No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4550.9197493430361836No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4380.8853850818677986No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4310.8712350919749343No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4190.8469779664443097No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4160.8409136850616535No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4100.8287851222963413No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4000.8085708510208208No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3880.7843137254901961No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3780.7640994542146755No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3640.7357994744289468No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3600.7277137659187386No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3500.7074994946432182No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3430.6933495047503537No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3320.6711138063472811No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3310.6690923792197291No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3230.6529209621993127No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3190.6448352536891044No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3190.6448352536891044No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3050.6165352739033758No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2820.5700424499696786No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2820.5700424499696786No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2710.547806751566606No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2700.5457853244390539No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2480.5013139276329088No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2380.48109965635738833No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2340.4730139478471801No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2320.468971093592076No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2250.4548211036992117No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2250.4548211036992117No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2240.45279967657165965No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2210.4467353951890034No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA2200.44471396806145136No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA2150.43460683242369114No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2100.42449969678593086No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA2100.42449969678593086No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2060.4164139882757227No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2020.4083282797655145No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1930.390135435617546No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1920.38811400848999394No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1870.37800687285223367No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1860.3759854457246816No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1840.3719425914695775No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1830.36992116434202543No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1800.3638568829593693No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1680.3395997574287447No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1670.33757833030119266No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1630.32949262179098443No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1610.32544976753588034No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1590.3214069132807762No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1560.31534263189812006No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1530.30927835051546393No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1530.30927835051546393No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1520.3072569233879119No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1520.3072569233879119No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1490.30119264200525575No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1460.29512836062259956No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1460.29512836062259956No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1460.29512836062259956No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1430.28906407923994343No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1340.2708712350919749No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1340.2708712350919749No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1330.26884980796442287No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1260.2546998180715585No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1240.2506569638164544No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1220.2466141095613503No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1220.2466141095613503No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1220.2466141095613503No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1160.234485546796038No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1160.234485546796038No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1150.23246411966848596No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1130.22842126541338187No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1130.22842126541338187No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1120.22639983828582982No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1120.22639983828582982No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1110.22437841115827772No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1080.2183141297756216No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1070.21629270264806955No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1050.21224984839296543No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1050.21224984839296543No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1020.2061855670103093No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1020.2061855670103093No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1020.2061855670103093No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1010.20416413988275725No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1010.20416413988275725No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT970.19607843137254902No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT970.19607843137254902No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT960.19405700424499697No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA900.18192844147968465No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT900.18192844147968465No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT890.1799070143521326No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT890.1799070143521326No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC860.17384273296947644No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA820.16575702445926824No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT810.1637355973317162No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT800.16171417020416412No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC790.15969274307661208No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT790.15969274307661208No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC780.15767131594906003No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT760.15362846169395594No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA750.1516070345664039No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA750.1516070345664039No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT740.14958560743885185No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT730.14756418031129978No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA720.14554275318374774No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA720.14554275318374774No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT700.14149989892864362No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA690.13947847180109157No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT690.13947847180109157No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA680.13745704467353953No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC670.13543561754598746No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC660.1334141904184354No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT660.1334141904184354No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT660.1334141904184354No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT640.1293713361633313No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT640.1293713361633313No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC630.12734990903577925No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT630.12734990903577925No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA620.1253284819082272No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA620.1253284819082272No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC620.1253284819082272No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC610.12330705478067515No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA600.1212856276531231No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA600.1212856276531231No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA590.11926420052557106No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT590.11926420052557106No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA590.11926420052557106No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.11926420052557106No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT580.117242773398019No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCT580.117242773398019No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT550.11117849201536284No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC540.1091570648878108No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.1091570648878108No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC530.10713563776025875No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT520.10511421063270669No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT510.10309278350515465No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC510.10309278350515465No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCT510.10309278350515465No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT500.1010713563776026No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACC500.1010713563776026No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA500.1010713563776026No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC500.1010713563776026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)