FastQCFastQC Report
Sun 21 May 2023
EGAF00007847948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48096
Sequences flagged as poor quality0
Sequence length250
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA26285.4640718562874255No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA24705.135562208915502No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG15813.287175648702595No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG15733.2705422488356617No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10132.106204258150366No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9031.8774950099800398No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8931.856703260146374No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8381.7423486360612108No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7211.4990851630073188No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG6331.316117764471058No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6261.3015635395874918No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6141.2766134397870925No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG6081.2641383898868928No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5911.2287924151696608No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5651.1747338656021291No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5351.1123586161011312No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5241.0894876912840985No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5191.0790918163672656No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4800.998003992015968No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4690.9751330671989353No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4690.9751330671989353No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4610.9584996673320026No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4100.852461743180306No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4070.8462242182302062No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4040.8399866932801064No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT4040.8399866932801064No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3790.7880073186959414No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3740.7776114437791084No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3630.7547405189620758No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3580.7443446440452428No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3530.7339487691284098No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3520.7318695941450433No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3440.7152361942781105No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3440.7152361942781105No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3260.6778110445775117No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3190.6632568196939455No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3150.654940119760479No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3020.6279108449767132No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3020.6279108449767132No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2990.6216733200266135No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2800.5821689953426481No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2760.5738522954091816No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2610.5426646706586826No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2530.5260312707917498No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2490.5177145708582834No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2460.5114770459081837No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2430.5052395209580838No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2430.5052395209580838No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2430.5052395209580838No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA2230.46365602129075184No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2210.45949767132401864No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2130.44286427145708585No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2120.4407850964737193No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2090.43454757152361945No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2080.4324683965402528No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2040.4241516966067864No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2000.41583499667332No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1940.4033599467731204No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1940.4033599467731204No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1930.40128077178975385No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1790.3721723220226214No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1770.36801397205588826No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1750.363855622089155No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1650.343063872255489No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1620.33682634730538924No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1610.3347471723220226No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1600.332667997338656No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1570.3264304723885562No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1530.3181137724550898No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1500.31187624750499005No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1470.3056387225548902No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1460.3035595475715236No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1460.3035595475715236No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1420.2952428476380572No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1420.2952428476380572No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1390.2890053226879574No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1380.2869261477045908No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1360.28276779773785765No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1320.27445109780439125No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1240.2578176979374584No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1230.25573852295409183No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1210.25158017298735863No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1200.249500998003992No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1180.24534264803725883No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1160.2411842980705256No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1160.2411842980705256No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1140.23702594810379243No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1140.23702594810379243No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1130.23494677312042583No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1130.23494677312042583No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1120.2328675981370592No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1120.2328675981370592No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1110.2307884231536926No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1090.2266300731869594No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1090.2266300731869594No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1070.22247172322022618No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1040.2162341982701264No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1020.2120758483033932No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1010.2099966733200266No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1000.20791749833666No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA990.20583832335329344No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA980.2037591483699268No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA980.2037591483699268No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT970.2016799733865602No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT960.19960079840319359No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT950.19752162341982704No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT950.19752162341982704No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT940.19544244843646041No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT860.17880904856952762No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA850.17672987358616102No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT840.17465069860279442No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT830.17257152361942782No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA830.17257152361942782No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT830.17257152361942782No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA810.16841317365269462No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA790.16425482368596142No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT780.1621756487025948No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT760.1580172987358616No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.1580172987358616No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT740.15385894876912842No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT740.15385894876912842No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA740.15385894876912842No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCT730.1517797737857618No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA720.14970059880239522No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCT720.14970059880239522No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.1476214238190286No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA700.14554224883566202No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT690.1434630738522954No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA690.1434630738522954No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA670.1393047238855622No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA640.1330671989354624No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT630.1309880239520958No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT630.1309880239520958No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCACGCT620.1289088489687292No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC620.1289088489687292No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA620.1289088489687292No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACGCT620.1289088489687292No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT610.1268296739853626No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA600.124750499001996No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTG600.124750499001996No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.12267132401862942No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT590.12267132401862942No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA570.11851297405189622No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT570.11851297405189622No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT570.11851297405189622No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT570.11851297405189622No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC560.1164337990685296No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCT550.11435462408516302No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA530.11019627411842982No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT530.11019627411842982No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTG520.1081170991350632No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCT520.1081170991350632No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCT520.1081170991350632No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA520.1081170991350632No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA520.1081170991350632No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT520.1081170991350632No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA510.1060379241516966No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT500.10395874916833No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTCGCTCCCCATGCT490.1018795741849634No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA490.1018795741849634No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT490.1018795741849634No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCACGCT490.1018795741849634No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCT490.1018795741849634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)