FastQCFastQC Report
Sun 21 May 2023
EGAF00007847958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65834
Sequences flagged as poor quality0
Sequence length250
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA50217.626758210043443No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA37515.697663821125862No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA35915.454628307561443No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA25873.9295804599447095No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT14242.1630160707233346No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG13662.074915697056232No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT11611.7635264453018198No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT11311.717957286508491No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10551.6025154175653917No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT10531.5994774736458366No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT10141.5402375672145092No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9821.4916304645016252No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT9401.4278336421909652No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT8611.307834857368533No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT8251.2531518668165387No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT7741.1756842968678798No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT7401.1240392502354406No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG7361.1179633623963303No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT6991.061761399884558No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6550.9949266336543426No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTA6200.941762615062126No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6160.9356867272230155No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT6150.9341677552632379No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6120.9296108393839049No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT5570.8460673815961358No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT5560.8445484096363581No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT5520.8384725217972476No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT4990.7579670079290337No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT4970.7549290640094783No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT4900.744296260291035No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4540.6896132697390406No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTA4520.6865753258194854No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4410.6698666342619316No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG4130.6273354193881581No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4060.6167026156697147No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3900.5923990643132728No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTA3900.5923990643132728No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3880.5893611203937176No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT3770.5726524288361636No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3670.5574627092383875No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT3660.5559437372786098No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT3580.5437919616003889No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3550.539235045721056No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT3530.5361971018015007No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3200.4860710271288392No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3130.4754382234103958No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3080.46784336361150775No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT3070.4663243916517301No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTA3060.46480541969195244No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2960.4496157000941763No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2850.43290700853662245No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2780.42227420481817907No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2780.42227420481817907No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2740.4161983169790686No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2730.41467934501929093No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2690.40860345718018043No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2600.39493270954218185No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT2540.3858188777835161No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2490.378224017984628No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2480.3767050460248504No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2390.3630342983868518No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG2340.3554394385879637No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2270.3448066348695203No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA2130.32354102743263363No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2120.32202205547285595No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2070.3144271956739679No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2050.3113892517544126No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2040.309870279794635No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2010.3053133639153021No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1940.2946805601968588No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1850.28100981255886015No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1780.2703770088404168No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1770.2688580368806392No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1740.2643011210013063No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT1720.26126317708175106No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG1710.25974420512197344No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1650.25063037336330773No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG1590.24151654160464198No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1560.2369596257253091No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1550.2354406537655315No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1480.22480785004708814No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1470.22328887808731052No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1450.22025093416775526No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1420.21569401828842238No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1380.2096181304493119No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT1360.20658018652975665No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1330.20202327065042378No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1290.1959473828113133No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1260.19139046693198045No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG1250.18987149497220282No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT1240.1883525230124252No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1230.18683355105264757No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT1190.18075766321353706No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1180.17923869125375946No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1160.1762007473342042No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1160.1762007473342042No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1160.1762007473342042No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1130.17164383145487136No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCT1100.16708691557553848No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT1090.16556794361576085No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1080.16404897165598323No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1050.15949205577665038No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1040.15797308381687275No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1020.1549351398973175No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1000.15189719597776224No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCT990.15037822401798462No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA990.15037822401798462No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT990.15037822401798462No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCT990.15037822401798462No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCT990.15037822401798462No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA970.1473402800984294No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT920.1397454202995413No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT910.13822644833976364No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA900.136707476379986No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT880.13366953246043078No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCT870.13215056050065316No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT850.1291126165810979No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT850.1291126165810979No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCT840.1275936446213203No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA840.1275936446213203No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA830.12607467266154268No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA790.11999878482243218No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCT790.11999878482243218No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT770.11696084090287694No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT770.11696084090287694No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA770.11696084090287694No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA760.11544186894309931No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT760.11544186894309931No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT750.1139228969833217No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCT750.1139228969833217No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT740.11240392502354407No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCT730.11088495306376646No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT720.1093659811039888No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT720.1093659811039888No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCT710.10784700914421119No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA710.10784700914421119No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT700.10632803718443357No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT690.10480906522465595No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCT680.10329009326487833No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT680.10329009326487833No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT670.10177112130510071No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)