FastQCFastQC Report
Sun 21 May 2023
EGAF00007847960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58946
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA18673.167305669595901No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA16502.7991721236385847No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA12052.044243884233027No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11461.944152274963526No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG9451.6031622162657349No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG9081.5403929019780815No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA8871.5047670749499542No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG8521.4453906965697418No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8511.4436942286160215No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7641.2961015166423506No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7541.2791368371051473No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7381.2519933498456215No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7221.2248498625860957No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7101.2044922471414514No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG7011.1892240355579684No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6641.126454721270315No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6621.1230617853628744No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6391.0840430224273063No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6271.0636854069826622No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6101.0348454517694161No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG5991.0161843042784922No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5830.9890408170189664No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5750.9754690733892037No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5680.9635937977131612No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5660.9602008618057205No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5550.9415397143147967No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5310.900824483425508No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5090.8635021884436603No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5040.8550198486750584No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4970.8431445729990161No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4810.8160010857394904No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4780.8109116818783293No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA4650.7888575984799646No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4600.7803752587113629No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4590.7786787907576426No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4550.7718929189427612No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4490.7617141112204391No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA4490.7617141112204391No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT4360.7396600278220745No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4360.7396600278220745No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT4310.7311776880534726No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4240.7193024123774302No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4200.7125165405625488No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3920.6650154378583789No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3760.6378719505988532No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3750.6361754826451328No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3710.6293896108302514No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3510.5954602517558443No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3470.5886743799409629No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3430.5818885081260815No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3340.5666202965425984No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3310.5615308926814372No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT3270.5547450208665559No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3250.5513520849591151No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT3170.5377803413293523No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3110.5276015336070302No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3100.5259050656533099No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT3010.5106368540698266No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3000.5089403861161063No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3000.5089403861161063No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2960.5021545143012248No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2960.5021545143012248No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2820.47840396294913984No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2700.45804634750449563No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2700.45804634750449563No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2610.44277813592101245No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT2600.4410816679672921No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2570.435992264106131No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2490.4224205204763682No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2460.4173311166152071No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2430.4122417127540461No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2390.40545584093916465No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2350.39866996912428326No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2330.3952770332168425No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2280.3867946934482408No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2240.38000882163335936No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA2200.3732229498184779No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT2200.3732229498184779No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2170.36813354595731684No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT2150.3647406100498761No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2120.3596512061887151No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT2020.34268652665151156No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2000.33929359074407084No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1970.33420418688290976No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1950.3308112509754691No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1920.325721847114308No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1900.3223289112068673No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1800.30536423166966375No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1730.2934889559936213No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1670.2833101482712992No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1530.2595595969192142No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1480.2510772571506124No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1480.2510772571506124No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1470.24938078919689208No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT1420.2408984494282903No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1400.2375055135208496No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1350.22902317375224782No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1290.2188443660299257No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1260.2137549621687646No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1200.2035761544464425No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT1160.19679028263156112No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT1160.19679028263156112No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1150.19509381467784073No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1140.1933973467241204No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1120.19000441081667968No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT1090.18491500695551863No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCT1020.17303973127947614No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTCGCTCCCCATGCT1010.17134326332575578No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1000.16964679537203542No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA990.16795032741831506No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC990.16795032741831506No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA960.162860923557154No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCT940.1594679876497133No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCT920.1560750517422726No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA920.1560750517422726No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC920.1560750517422726No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT920.1560750517422726No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCT900.15268211583483188No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA890.15098564788111152No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT870.1475927119736708No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA830.1408068401587894No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA830.1408068401587894No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC830.1408068401587894No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA830.1408068401587894No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.1374139042513487No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA810.1374139042513487No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC790.13402096834390798No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.13232450039018762No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA770.13062803243646726No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAACACGTAGGGTGCGAGCGTTA770.13062803243646726No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA770.13062803243646726No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT770.13062803243646726No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.12893156448274692No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA740.1255386285753062No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA740.1255386285753062No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA730.12384216062158585No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT720.12214569266786551No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA710.12044922471414515No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.12044922471414515No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.1187527567604248No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCT690.11705628880670443No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA670.11366335289926374No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT670.11366335289926374No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT660.11196688494554338No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC640.10857394903810266No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA640.10857394903810266No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT630.1068774810843823No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCT620.10518101313066197No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA620.10518101313066197No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAACACGTAGGGTGCGAGCGTTA620.10518101313066197No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.10348454517694161No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC600.10178807722322125No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT600.10178807722322125No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)