FastQCFastQC Report
Sun 21 May 2023
EGAF00007847961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58946
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA20423.4641875614969635No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA18913.2080209004851894No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA12382.1002273267057987No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11972.030672140603264No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG9991.694771485766634No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG9361.5878940046822516No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9091.542089369931802No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT8671.470837715875547No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8641.4657483120143862No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8421.4284260170325382No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8021.360567298883724No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7981.3537814270688426No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA7651.297797984596071No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG7601.2893156448274692No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7411.2570827537067824No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7231.226546330539816No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG6371.0806500865198656No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6291.0670783428901027No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG6281.0653818749363826No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT6051.0263631120008143No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5911.0026125606487293No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5890.9992196247412887No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5880.9975231567875683No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5740.9737726054354834No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5530.9381467784073558No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5420.9194856309164321No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5400.9160926950089913No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5190.8804668679808638No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5180.8787704000271435No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4930.8363587011841346No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4920.8346622332304142No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4650.7888575984799646No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4590.7786787907576426No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA4430.7515353034981169No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4400.7464458996369558No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4360.7396600278220745No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4210.7142130085162691No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4150.704034200793947No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA4030.6836765853493028No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3980.675194245580701No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3970.6734977776269806No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3930.6667119058120993No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3880.6582295660434975No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3760.6378719505988532No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3690.6259966749228107No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3640.617514335154209No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3630.6158178672004886No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3600.6107284633393275No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3460.5869779119872426No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3400.5767991042649204No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3310.5615308926814372No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3290.5581379567739966No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3240.5496556170053948No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3230.5479591490516744No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3180.5394768092830726No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2960.5021545143012248No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2920.4953686424863434No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2890.49027923862518236No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2860.4851898347640213No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2800.4750110270416992No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2750.4665286872730974No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2740.46483221931937707No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2740.46483221931937707No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2710.45974281545821605No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2660.4512604756896142No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2520.4275099243375292No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2470.41902758456892747No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2460.4173311166152071No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT2440.41393818070776645No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2410.40884877684660537No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2380.40375937298544434No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2350.39866996912428326No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2280.3867946934482408No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2270.3850982254945204No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2170.36813354595731684No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2160.36643707800359654No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2120.3596512061887151No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2120.3596512061887151No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT2110.35795473823499474No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2050.3477759305126726No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1930.3274183150680284No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1920.325721847114308No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1900.3223289112068673No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1860.31554303939198586No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1800.30536423166966375No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1740.2951854239473416No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1730.2934889559936213No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1720.29179248803990093No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1640.27822074441013805No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1540.2612560648729345No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1490.2527737251043328No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1480.2510772571506124No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1400.2375055135208496No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1400.2375055135208496No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1400.2375055135208496No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1370.23241610965968854No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1350.22902317375224782No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1310.22223730193736638No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1300.22054083398364605No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1290.2188443660299257No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1270.215451430122485No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTCGCTCCCCATGCT1240.21036202626132394No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1230.20866555830760355No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT1220.20696909035388322No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1160.19679028263156112No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT1130.19170087877040004No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1110.18830794286295932No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1110.18830794286295932No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1040.17643266718691686No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1010.17134326332575578No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC980.16625385946459473No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC980.16625385946459473No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA970.16455739151087437No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT960.162860923557154No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA950.16116445560343365No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT950.16116445560343365No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT930.15777151969599293No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC920.1560750517422726No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCT900.15268211583483188No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA890.15098564788111152No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA890.15098564788111152No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA870.1475927119736708No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA830.1408068401587894No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA800.13571743629762834No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT790.13402096834390798No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA790.13402096834390798No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA780.13232450039018762No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA760.12893156448274692No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA730.12384216062158585No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA710.12044922471414515No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT710.12044922471414515No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA710.12044922471414515No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA700.1187527567604248No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC680.11535982085298407No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA670.11366335289926374No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA670.11366335289926374No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCT670.11366335289926374No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA670.11366335289926374No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA670.11366335289926374No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT660.11196688494554338No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA660.11196688494554338No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC640.10857394903810266No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.10857394903810266No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT640.10857394903810266No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG630.1068774810843823No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT630.1068774810843823No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCT620.10518101313066197No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCT620.10518101313066197No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA620.10518101313066197No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAACACGTAGGGTGCGAGCGTTA620.10518101313066197No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAACACGTAGGGTGCGAGCGTTA620.10518101313066197No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.10348454517694161No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT610.10348454517694161No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA610.10348454517694161No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA590.10009160926950089No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC590.10009160926950089No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)