FastQCFastQC Report
Sun 21 May 2023
EGAF00007847974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59810
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA25514.265173047985287No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA23613.9475004179903026No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA17302.8924928941648553No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA15312.559772613275372No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10891.8207657582344088No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT9541.59505099481692No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9411.5733154990804212No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT8321.3910717271359305No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT7741.2940979769269354No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7741.2940979769269354No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7631.2757064036114363No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7121.1904363818759405No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7111.1887644206654406No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT6751.1285738170874435No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT6741.1269018558769437No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6731.1252298946664436No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT6711.121885972245444No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT5960.9964888814579501No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5570.9312823942484535No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA5430.9078749373014547No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT5420.9062029760909547No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT5100.8527002173549573No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT4890.8175890319344591No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4810.8042133422504597No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4660.779133924092961No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4660.779133924092961No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4630.7741180404614613No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4530.7573984283564621No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4280.7155993980939642No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT4280.7155993980939642No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4270.7139274368834643No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT4040.6754723290419662No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3900.6520648720949673No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3870.6470489884634677No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT3840.6420331048319678No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3840.6420331048319678No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3810.6370172212004681No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT3710.6202976090954689No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3490.5835144624644708No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3420.5718107339909714No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3390.5667948503594716No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT3380.5651228891489718No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3300.5517471994649724No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3250.5433873934124729No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3250.5433873934124729No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT3190.5333556261494734No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3090.5166360140444742No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3020.5049322855709747No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2970.49657247951847516No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2950.4932285570974753No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2840.4748369837819763No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2770.4631332553084768No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2670.44641364320347765No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT2640.44139775957197797No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2630.43972579836147796No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2610.43638187594047817No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT2550.42635010867747863No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2520.42133422504597895No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2500.4179903026249791No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2480.41464638020397926No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2430.40628657415147973No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2310.3862230396254807No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2310.3862230396254807No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT2300.3845510784149808No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2150.359471660257482No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2080.34776793178398263No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2080.34776793178398263No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2070.3460959705734827No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2050.34275204815248284No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2020.3377361645209831No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1890.31600066878448424No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1820.3042969403109848No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1790.29928105667948507No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1780.2976090954689851No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1750.2925932118374854No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1750.2925932118374854No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1750.2925932118374854No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1720.28757732820598564No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1670.27921752215348605No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1650.2758735997324862No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1600.2675137936799866No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1560.26082594883798693No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1530.2558100652064872No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1490.24912222036448753No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1480.2474502591539876No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1420.23741849189098815No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1400.2340745694699883No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1380.23073064704898846No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1370.22905868583848857No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1370.22905868583848857No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1350.22571476341748872No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1350.22571476341748872No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1320.22069887978598898No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1260.21066711252298947No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1240.20732319010198963No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1210.2023073064704899No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1210.2023073064704899No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1180.19729142283899012No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1180.19729142283899012No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1170.1956194616284902No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1150.1922755392074904No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1130.18893161678649056No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1120.18725965557599064No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1120.18725965557599064No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1110.18558769436549072No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1100.1839157331549908No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1100.1839157331549908No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1100.1839157331549908No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1080.18057181073399098No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1070.17889984952349106No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1060.17722788831299113No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1060.17722788831299113No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1050.1755559271024912No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.1755559271024912No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1020.17054004347099147No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1010.16886808226049155No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1010.16886808226049155No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1000.16719612104999163No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA980.1638521986289918No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC980.1638521986289918No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA950.15883631499749204No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA940.15716435378699214No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC940.15716435378699214No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT930.15549239257649222No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT930.15549239257649222No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA880.14713258652399264No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG880.14713258652399264No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC880.14713258652399264No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT860.14378866410299282No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA850.1421167028924929No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT840.14044474168199297No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCT830.13877278047149305No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA820.13710081926099316No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC820.13710081926099316No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA810.13542885805049323No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCT810.13542885805049323No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT810.13542885805049323No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC790.1320849356294934No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA770.12874101320849354No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA770.12874101320849354No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG760.12706905199799365No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA760.12706905199799365No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT740.1237251295769938No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT720.12038120715599399No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA710.11870924594549408No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT700.11703728473499415No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA700.11703728473499415No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA690.11536532352449423No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT680.11369336231399432No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA670.1120214011034944No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC670.1120214011034944No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC650.10867747868249457No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT630.10533355626149474No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC620.10366159505099481No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCT610.10198963384049489No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA600.10031767262999498No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA600.10031767262999498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)