FastQCFastQC Report
Sun 21 May 2023
EGAF00007847975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59810
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA25404.246781474669787No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA25174.20832636682829No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA16162.7018893161678648No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA16142.698545393746865No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10581.7689349607089115No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT9931.660257482026417No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT9261.5482360809229225No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9251.5465641197124227No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8501.421167028924929No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT8351.3960876107674303No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT7581.2673465975589366No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7381.2339073733489383No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7041.177060692191941No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6921.1569971576659421No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6341.060023407456947No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5991.00150476508945No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA5850.9780973081424511No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5670.9480020063534526No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT5430.9078749373014547No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5290.8844674803544558No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT5250.8777796355124561No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5230.8744357130914563No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA5220.8727637518809563No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT4970.8309647216184584No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT4750.7941815749874603No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4690.7841498077244607No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4530.7573984283564621No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4300.718943320514964No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4160.6955358635679653No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT4080.6821601738839659No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3900.6520648720949673No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3900.6520648720949673No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3890.6503929108844675No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3800.6353452599899682No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3760.6286574151479686No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3730.6236415315164688No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3720.6219695703059689No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3610.6035779969904698No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3460.5784985788329711No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3440.5751546564119713No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT3440.5751546564119713No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3420.5718107339909714No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA3350.5601070055174721No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3340.5584350443069721No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT3290.5500752382544726No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3150.5266677813074737No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3110.519979936465474No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2940.4915565958869755No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2890.48319678983447584No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2830.4731650225714763No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2750.459789332887477No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2740.45811737167697714No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2690.4497575656244775No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2580.43136599230897843No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT2570.4296940310984785No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2570.4296940310984785No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2440.40795853536197957No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2440.40795853536197957No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2440.40795853536197957No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2400.40127069051997993No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2370.3962548068884802No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2280.381207155993981No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2270.379535194783481No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2230.3728473499414814No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2210.36950342752048154No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2200.3678314663099816No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT2170.36281558267848185No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2110.35278381541548237No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2100.3511118542049824No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2030.33940812573148305No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2020.3377361645209831No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1940.32436047483698377No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1940.32436047483698377No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1930.3226885136264839No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1920.3210165524159839No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1860.31098478515298444No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1830.3059689015214847No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1830.3059689015214847No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1790.29928105667948507No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1790.29928105667948507No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1790.29928105667948507No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1780.2976090954689851No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1770.2959371342584852No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1700.2842334057849858No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1640.2742016385219863No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1630.27252967731148636No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1590.26584183246948667No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1530.2558100652064872No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1500.25079418157498745No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1460.24410633673298782No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1450.2424343755224879No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1390.23240260825948839No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1380.23073064704898846No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1380.23073064704898846No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1380.23073064704898846No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1290.21568299615448921No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1290.21568299615448921No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1280.2140110349439893No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1270.21233907373348937No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1260.21066711252298947No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1260.21066711252298947No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1220.20397926768098978No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1200.20063534525998997No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1160.1939475004179903No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1150.1922755392074904No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1140.1906035779969905No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1110.18558769436549072No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1110.18558769436549072No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1100.1839157331549908No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1070.17889984952349106No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1060.17722788831299113No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1050.1755559271024912No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1040.17388396589199132No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1040.17388396589199132No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1030.1722120046814914No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1010.16886808226049155No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1010.16886808226049155No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1010.16886808226049155No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1010.16886808226049155No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1000.16719612104999163No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA980.1638521986289918No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT950.15883631499749204No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA950.15883631499749204No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT940.15716435378699214No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA930.15549239257649222No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA930.15549239257649222No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA920.15382043136599233No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT890.14880454773449256No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT880.14713258652399264No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT870.14546062531349274No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA860.14378866410299282No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA850.1421167028924929No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA850.1421167028924929No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA840.14044474168199297No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA830.13877278047149305No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA810.13542885805049323No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC810.13542885805049323No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT790.1320849356294934No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC790.1320849356294934No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT780.13041297441899347No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA770.12874101320849354No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC770.12874101320849354No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC770.12874101320849354No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA770.12874101320849354No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA750.12539709078749373No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT750.12539709078749373No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA750.12539709078749373No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA730.12205316836649391No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA700.11703728473499415No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA700.11703728473499415No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.11703728473499415No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA680.11369336231399432No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA680.11369336231399432No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG670.1120214011034944No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG650.10867747868249457No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCT640.10700551747199465No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT630.10533355626149474No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC620.10366159505099481No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT610.10198963384049489No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT600.10031767262999498No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC600.10031767262999498No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC600.10031767262999498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)