FastQCFastQC Report
Sun 21 May 2023
EGAF00007847980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52840
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA22004.163512490537472No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA19273.6468584405753215No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA15612.954201362604088No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA14132.674110522331567No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10371.9625283875851627No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8971.6975775927327783No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7951.504542013626041No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7351.390991672975019No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7141.3512490537471613No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6471.2244511733535202No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6351.2017411052233158No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6161.1657834973504921No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6131.1601059803179408No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT6101.1544284632853898No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5831.10333080999243No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5791.0957607872823618No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5711.0806207418622256No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5541.048448145344436No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5431.0276305828917487No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5301.0030280090840271No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5120.9689629068887207No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4780.9046177138531416No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT4770.9027252081756245No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4660.8819076457229371No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4550.8610900832702498No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT4520.8554125662376988No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4290.811884935654807No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4250.8043149129447388No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4250.8043149129447388No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4200.7948523845571537No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4070.7702498107494323No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4000.757002271006813No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3980.7532172596517789No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3940.7456472369417109No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3720.7040121120363362No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3710.7021196063588191No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3410.6453444360333082No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3360.6358819076457229No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3340.6320968962906889No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3280.6207418622255867No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT3260.6169568508705526No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3240.6131718395155186No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3210.6074943224829674No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3190.6037093111279335No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3150.5961392884178652No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3100.58667676003028No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3040.5753217259651779No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2870.5431491294473884No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2770.524224072672218No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2590.4901589704769114No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2570.4863739591218773No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2540.48069644208932627No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2520.47691143073429226No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2470.46744890234670705No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2460.46555639666918996No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2400.45420136260408783No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2330.44095382286146856No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2320.4390613171839515No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2320.4390613171839515No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2290.4333838001514005No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2260.4277062831188494No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT2250.4258137774413323No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT2170.41067373202119606No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2110.39931869795609387No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2090.39553368660105975No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2020.3822861468584406No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2020.3822861468584406No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2020.3822861468584406No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2000.3785011355034065No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1980.37471612414837246No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1940.36714610143830434No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1920.3633610900832702No Hit
TCGGAAGGCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1890.3576835730507192No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1890.3576835730507192No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1860.3520060560181681No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1850.35011355034065106No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1820.34443603330809996No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1770.33497350492051475No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1770.33497350492051475No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1760.3330809992429977No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1760.3330809992429977No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1720.3255109765329296No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1670.31604844814534444No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1630.3084784254352763No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1610.30469341408024225No Hit
TCGGAAGGCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1560.2952308856926571No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1540.29144587433762303No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1520.28766086298258897No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1450.27441332323996975No Hit
TCGGAAGGCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1340.2535957607872824No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1310.24791824375473126No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1300.24602573807721426No Hit
TCGGAAGGCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1300.24602573807721426No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1270.24034822104466314No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1260.23845571536714613No Hit
TCGGAAGGCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1260.23845571536714613No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1250.23656320968962907No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1240.23467070401211204No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1230.23277819833459498No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA1200.22710068130204392No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1160.21953065859197576No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1150.21763815291445873No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1120.21196063588190764No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1110.2100681302043906No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1090.20628311884935657No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1090.20628311884935657No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.19871309613928842No Hit
TCGGAAGGCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1050.19871309613928842No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1010.1911430734292203No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1000.18925056775170326No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA990.18735806207418623No Hit
TCGGAAGGCCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT980.1854655563966692No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC970.18357305071915217No Hit
TCGGAAGGCCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT960.1816805450416351No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA960.1816805450416351No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC950.1797880393641181No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA950.1797880393641181No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC940.17789553368660105No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA930.17600302800908404No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC920.17411052233156699No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA920.17411052233156699No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT910.17221801665404998No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA840.15897047691143074No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA800.1514004542013626No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT800.1514004542013626No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT770.14572293716881152No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA750.14193792581377745No Hit
TCGGAAGGCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT750.14193792581377745No Hit
TCGGAAGGCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC740.1400454201362604No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA710.13436790310370933No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG710.13436790310370933No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT690.13058289174867527No Hit
TCGGAAGGCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC680.1286903860711582No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.1267978803936412No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA670.1267978803936412No Hit
TCGGAAGGCCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT660.12490537471612415No Hit
TCGGAAGGCCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT660.12490537471612415No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG660.12490537471612415No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA660.12490537471612415No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC660.12490537471612415No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA660.12490537471612415No Hit
TCGGAAGGCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT650.12301286903860713No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT640.12112036336109008No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA620.11733535200605602No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA620.11733535200605602No Hit
TCGGAAGGCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC620.11733535200605602No Hit
TCGGAAGGCCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT610.11544284632853899No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA610.11544284632853899No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA600.11355034065102196No Hit
TCGGAAGGCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT600.11355034065102196No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT600.11355034065102196No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA590.11165783497350493No Hit
TCGGAAGGCCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT590.11165783497350493No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT580.10976532929598788No Hit
TCGGAAGGCCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT570.10787282361847086No Hit
TCGGAAGGCCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.10598031794095382No Hit
TCGGAAGGCCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT550.1040878122634368No Hit
TCGGAAGGCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT540.10219530658591976No Hit
TCGGAAGGCCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC530.10030280090840274No Hit
TCGGAAGGCCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT530.10030280090840274No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)