FastQCFastQC Report
Sun 21 May 2023
EGAF00007847989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100457
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA67236.692415660431826No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA47344.712464039340215No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA36273.610500014931762No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA26922.679753526384423No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA24992.4876315239356144No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT22662.2556914898911975No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT19671.9580517037140268No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA18381.8296385518181908No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT16851.6773345809649902No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG16271.6195984351513582No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT15671.5598713877579462No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT15441.5369760195904716No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA15031.4961625372049732No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT14521.445394546920573No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT13471.3408722139821017No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT11871.1816000875996695No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT11201.114904884677026No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG11081.1029594751983436No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT10781.0730959515016374No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA10000.9954507898902019No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9900.9854962819912997No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA9880.9835053804115195No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT9830.9785281264620683No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9220.917805628278766No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT8960.8919239077416208No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT8760.8720148919438167No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8620.8580785808853538No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT8590.8550922285156833No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT8510.8471286221965617No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT8440.8401604666673304No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8150.8112923937605144No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG7790.7754561653244672No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7660.7625153050558946No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG7500.7465880924176513No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTA7230.7197109210906159No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT6870.6838746926545686No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6460.6430612102690704No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT6300.6271339976308271No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT6280.6251430960510467No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6200.6171794897319252No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT6160.6131976865723643No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5960.5932886707745603No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT5860.5833341628756582No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT5750.572384204186866No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5680.5654160486576346No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG5590.5564569915486228No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5560.5534706391789522No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT5440.5415252297002697No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5260.5236071154822461No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5100.5076799028440029No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5100.5076799028440029No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA4860.4837890838866381No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4810.478811829937187No Hit
TGTGTTAAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT4750.4728391251978458No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT4690.4668664204585046No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4680.4658709696686144No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTA4530.45093920782026137No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT4440.44198015071124963No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG4220.4200802333336652No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3960.39419851279651985No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3900.3882258080571787No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3810.37926675094816686No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTA3780.37628039857849627No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3760.3742894969987159No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3630.3613486367301432No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT3600.3583622843604726No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT3510.34940322725146084No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT3440.3424350717222294No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA3390.3374578177727784No Hit
TGTGTTAAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT3190.31754880197497437No Hit
TGTGTTAAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT3140.31257154802552334No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3010.29963068775695073No Hit
TGTGTTAAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT2740.2727535164299153No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2580.25682630379167204No Hit
TGTGTTAAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2570.2558308530017819No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT2540.2528445006321113No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2490.24786724668266025No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTA2410.23990364036353862No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2380.23691728799386802No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA2380.23691728799386802No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2370.2359218372039778No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2330.23194003404441704No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2320.23094458325452683No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2180.217008272196064No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2170.21601282140617378No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2110.2100401166668326No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2100.20904466587694237No Hit
TGTGTTAAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2080.207053764297162No Hit
TGTGTTAAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT2020.20108105955782074No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1940.19311745323869914No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1930.19212200244880895No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1900.18913565007913835No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1900.18913565007913835No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1870.18614929770946773No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1840.18316294533979713No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT1810.18017659297012653No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1790.17818569139034612No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1750.1742038882307853No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1720.1712175358611147No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1710.1702220850712245No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1690.16823118349144411No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA1640.16325392954199308No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1590.15827667559254208No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1580.1572812248026519No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1570.15628577401276167No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT1560.15529032322287148No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1540.15329942164309107No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1530.15230397085320088No Hit
TGTGTTAAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1510.15031306927342047No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1490.14832216769364007No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1490.14832216769364007No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT1480.14732671690374988No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1450.14434036453407925No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT1430.14234946295429884No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1380.13737220900484784No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1320.13139950426550664No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1270.12642225031605564No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1250.12443134873627523No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1190.11845864399693401No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1180.11746319320704382No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1180.11746319320704382No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1180.11746319320704382No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1170.1164677424171536No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1170.1164677424171536No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1150.1144768408373732No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1150.1144768408373732No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC1140.113481390047483No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1120.1114904884677026No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1120.1114904884677026No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1120.1114904884677026No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCT1120.1114904884677026No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1080.10750868530814178No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1080.10750868530814178No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCT1070.10651323451825159No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC1070.10651323451825159No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1070.10651323451825159No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1070.10651323451825159No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1060.10551778372836138No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1060.10551778372836138No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.10452233293847119No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1030.10253143135869078No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1010.10054052977891037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)