FastQCFastQC Report
Sun 21 May 2023
EGAF00007847998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58178
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA49798.558217883048574No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA46668.020213826532366No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA27404.709684073017292No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA26684.58592595139056No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT14912.56282443535357No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT14042.413283371721269No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT12062.0729485372477567No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT11161.9182508852143423No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA10691.8374643335968923No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT10411.7893361751864967No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA10291.7687098215820412No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT10201.7532400563787No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT8911.5315067551308055No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT7371.2668018838736292No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT7051.2117982742617484No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT6691.1499192134483824No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT6541.1241362714428134No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6521.1206985458420708No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT6351.0914778782357593No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6171.0605383478290762No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT6131.0536628966275914No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5560.9556877170064285No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5210.895527518993434No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT4750.8164598301763554No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4750.8164598301763554No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4690.8061466533741277No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT4490.7717693973667022No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT4470.7683316717659596No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4200.7219223761559352No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT4120.7081714737529651No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4030.6927017085496235No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3990.6858262573481384No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3930.6755130805459109No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3830.6583244525421981No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3790.651449001340713No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3700.6359792361373715No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3600.6187906081336587No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3420.5878510777269758No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3340.5741001753240056No Hit
TGTGTTAAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT3140.5397229193165802No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3060.52597201691361No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2980.5122211145106398No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2870.49331362370655574No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2860.4915947609061845No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2820.48471930970469934No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT2650.45549864209838764No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2610.4486231908969026No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2590.44518546529616004No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2590.44518546529616004No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2440.41940252329059097No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2380.4090893464883633No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2370.40737048368799206No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2230.3833064044827942No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2220.3815875416824229No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2200.37814981608168036No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2190.3764309532813091No Hit
TGTGTTAAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT2180.3747120904809378No Hit
TGTGTTAAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2180.3747120904809378No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2110.3626800508783389No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2070.3558045996768538No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2050.3523668740761112No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2040.35064801127573997No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1820.3128330296675719No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1740.29908212726460176No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1670.2870500876620028No Hit
TGTGTTAAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1610.2767369108597752No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1490.2561105572553199No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1480.25439169445494864No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1470.2526728316545773No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1450.24923510605383475No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1440.24751624325346352No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1420.24407851765272096No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1320.22688988964900825No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1310.22517102684863696No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1270.21829557564715185No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1220.20970126164529548No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1090.1873560452404689No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1060.1821994568393551No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.17876173123861253No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1010.17360514283749873No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA990.17016741723675616No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA990.17016741723675616No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA960.16501082883564233No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC950.16329196603527107No Hit
TGTGTTAAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT950.16329196603527107No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT940.1615731032348998No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT910.15641651483378596No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC870.14954106363230088No Hit
TGTGTTAAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT870.14954106363230088No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA870.14954106363230088No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA830.14266561243081577No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA830.14266561243081577No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA830.14266561243081577No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC830.14266561243081577No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.13922788683007323No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA760.13063357282821686No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA750.12891471002784557No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT740.12719584722747432No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT740.12719584722747432No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.12547698442710303No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA720.12375812162673176No Hit
TGTGTTAAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT700.1203203960259892No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA690.11860153322561792No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC680.11688267042524667No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA670.11516380762487538No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA660.11344494482450412No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT650.11172608202413284No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA630.10828835642339028No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT630.10828835642339028No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA620.106569493623019No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.10485063082264774No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT590.10141290522190519No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)