FastQCFastQC Report
Sun 21 May 2023
EGAF00007848013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46072
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA26735.801788504948776No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA15393.340423684667477No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA15123.2818197603750656No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT9111.9773398159402673No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9051.9643167216530648No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA8281.7971870116339643No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT8011.7385830873415522No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7221.5671123458933844No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT6371.3826185101580135No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6061.3153325230074666No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5981.2979683972911964No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5891.2784337558603924No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5801.2588991144295885No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5731.243705504427852No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5461.1851015801354403No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4871.0570411529779475No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4851.05270012154888No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4661.0114603229727384No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4560.9897551658274005No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4510.9789025872547317No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4200.9116166001041848No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4130.8964229901024483No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3930.8530126758117729No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3920.850842160097239No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3600.7813856572321584No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3600.7813856572321584No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3510.7618510158013544No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3510.7618510158013544No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3430.7444868900850842No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3240.7032470915089425No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3230.7010765757944087No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3220.6989060600798749No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3220.6989060600798749No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3170.6880534815072061No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2980.6468136829310643No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2950.6403021357874631No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2830.6142559472130579No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2690.5838687272095849No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2680.5816982114950512No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2670.5795276957805174No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2670.5795276957805174No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2600.5643340857787811No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2570.5578225386351797No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2520.5469699600625109No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2490.5404584129189096No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2470.536117381489842No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2460.5339468657753083No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2420.5252648029171731No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2380.516582740059038No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2340.5079006772009029No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2240.4861955200555652No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2210.47968397291196385No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2180.4731724257683626No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2160.468831394339295No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2010.4362736586212885No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1970.4275915957631533No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1970.4275915957631533No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1960.4254210800486195No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1960.4254210800486195No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1960.4254210800486195No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1940.421080048619552No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1840.39937489147421423No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1790.38852231290154543No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1730.37549921861434277No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1710.3711581871852752No Hit
TGTGTTAAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1710.3711581871852752No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1670.3624761243271401No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1610.34945303003993744No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1610.34945303003993744No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1550.33642993575273483No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1530.3320889043236673No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1470.31906581003646467No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1460.31689529432193086No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1440.31255426289286337No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1430.31038374717832956No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1350.2930196214620594No Hit
TGTGTTAAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1330.28867859003299184No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1320.28650807431845804No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1310.2843375586039243No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1300.28216704288939054No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1290.27999652717485674No Hit
TGTGTTAAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1270.2756554957457892No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1250.2713144643167216No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1240.2691439486021879No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1230.2669734328876541No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1220.2648029171731203No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1220.2648029171731203No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1210.2626324014585866No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1160.25177982288591766No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1150.24960930717138394No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1150.24960930717138394No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1110.24092724431324883No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1100.23875672859871502No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1060.23007466574057997No Hit
TGTGTTAAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1060.23007466574057997No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1040.2257336343115124No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1040.2257336343115124No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1030.22356311859697864No Hit
TGTGTTAAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1020.22139260288244486No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1010.21922208716791108No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA990.21488105573884356No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT960.20836950859524223No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA950.20619899288070845No Hit
TGTGTTAAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT930.2018579614516409No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA920.19968744573710712No Hit
TGTGTTAAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT910.19751693002257337No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC900.1953464143080396No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA890.1931758985935058No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT880.19100538287897206No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA870.18883486716443826No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC870.18883486716443826No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT860.1866643514499045No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA820.1779822885917694No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG820.1779822885917694No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA820.1779822885917694No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA810.17581177287723562No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA790.17147074144816807No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.16930022573363432No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC770.16712971001910054No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC770.16712971001910054No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT770.16712971001910054No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT740.1606181628754992No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC700.15193610001736413No Hit
TGTGTTAAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT700.15193610001736413No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC690.14976558430283035No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT680.14759506858829657No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT670.1454245528737628No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC670.1454245528737628No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAACACGTAAGGTGCGAGCGTTG670.1454245528737628No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAACACGTAAGGTGCGAGCGTTG670.1454245528737628No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC660.14325403715922902No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC630.13674249001562772No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA620.13457197430109394No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA620.13457197430109394No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG610.13240145858656016No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA610.13240145858656016No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT610.13240145858656016No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC600.13023094287202638No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA590.12806042715749263No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC590.12806042715749263No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT580.12588991144295883No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT570.12371939572842507No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA570.12371939572842507No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT540.11720784858482375No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT540.11720784858482375No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC540.11720784858482375No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA530.11503733287028998No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC510.11069630144122243No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT510.11069630144122243No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT510.11069630144122243No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT510.11069630144122243No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA510.11069630144122243No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT500.10852578572668867No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT500.10852578572668867No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA500.10852578572668867No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT490.10635527001215488No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA490.10635527001215488No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC470.10201423858308735No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)