FastQCFastQC Report
Sun 21 May 2023
EGAF00007848016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79279
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA53416.736966914315266No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA37514.731391667402464No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA24653.1092723167547525No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG23432.9553854110167888No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA17502.207394139683901No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA16702.1064846932983516No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG16672.1027005890588932No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT14811.8680861262124902No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT14381.8138472987802572No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA13531.7066310119956105No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG12821.617073878328435No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT12481.5741873636145765No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT12311.552744106257647No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT12251.5451758977787309No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA11901.5010280149850528No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG11441.4430050833133619No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT11301.4253459301958906No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT10401.311822803012147No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT10021.263890815979011No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9911.2500157671009977No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9701.223527037424791No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9611.2121747247064167No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA9151.1541517930347256No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT8871.1188334867997831No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT8521.074685604006105No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG8131.0254922488931495No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7961.0040489915362203No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7951.0027876234564008No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA7710.9725147895407359No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT7610.9599011087425422No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT6980.8804349197139217No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT6730.8489007177184374No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6560.8274574603615081No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6280.7921391541265657No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6240.7870936818072882No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT6210.7833095775678302No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT6150.7757413690889139No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT6140.7744800010090945No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6090.7681731606099976No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT6050.7631276882907202No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA5740.7240252778163196No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT5740.7240252778163196No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT5430.6849228673419191No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5330.6723091865437254No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT5270.6647409780648091No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5220.6584341376657122No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4980.6281613037500473No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT4800.6054566783132986No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT4740.5978884698343824No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4630.5840134209563693No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4590.5789679486370918No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT4210.5310359616039557No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT4110.5184222808057619No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT4020.5070699680873876No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3970.5007631276882908No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3830.48310397457081955No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3720.46922892569280644No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT3640.45913798105425147No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3580.45156977257533526No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3430.43264925137804466No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT3300.4162514663403928No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3230.4074218897816572No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3200.4036377855421991No Hit
TGTGTTAAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT2940.37084221546689544No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2900.3657967431476179No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2780.3506603261897855No Hit
TGTGTTAAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2580.325432964593398No Hit
TGTGTTAAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT2540.32038749227412056No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2530.31912612419430114No Hit
TGTGTTAAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2450.30903517955574616No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT2340.29516013067773306No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2310.291376026438275No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT2300.2901146583584556No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2250.2838078179593587No Hit
TGTGTTAAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT2100.2648872967620681No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1980.2497508798042357No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1970.2484895117244163No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1910.2409213032455001No Hit
TGTGTTAAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1870.2358758309262226No Hit
TGTGTTAAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1770.22326215012802886No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1560.19677342045182206No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1560.19677342045182206No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1540.1942506842921833No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1490.18794384389308644No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1400.1765915311747121No Hit
TGTGTTAAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1310.16523921845633777No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1260.1589323780572409No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1180.14884143341868591No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1160.14631869725904717No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1120.14127322493976968No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1110.14001185685995032No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1100.13875048878013094No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1070.13496638454067283No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1010.12739817606175657No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1000.12613680798193722No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC990.12487543990211784No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA990.12487543990211784No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT950.11982996758284035No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA930.11730723142320161No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT920.11604586334338224No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA910.11478449526356287No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA890.11226175910392412No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT860.108477654864466No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA830.10469355062500789No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA830.10469355062500789No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTG820.10343218254518852No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT820.10343218254518852No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC810.10217081446536914No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)