FastQCFastQC Report
Sun 21 May 2023
EGAF00007848026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49564
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA21674.372124929384231No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA18363.7043015091598743No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG15613.149463320151723No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA15203.066741990154144No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG14612.947703978694214No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA13292.681381647970301No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA8901.7956581389718345No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8231.660479380195303No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG8171.6483738197078526No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT7151.4425792914211928No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7101.4324913243483173No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6511.3134533128883867No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6341.27915422484061No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6011.212573642159632No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5831.1762569606972801No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5611.1318699055766281No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5341.0773948833831006No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5331.0753772899685254No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5241.0572189492373496No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4850.978532806068921No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4720.9523040916794447No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4660.9401985311919941No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4640.9361633443628441No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4550.918005003631668No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4360.8796707287547414No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4340.8756355419255911No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4090.8251957065612138No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3860.7787910580259866No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3740.7545799370510854No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3450.6960697280284077No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3440.6940521346138326No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3310.6678234202243564No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3310.6678234202243564No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3090.6234363651037044No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3000.6052780243725284No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2930.5911548704705027No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2850.575014123153902No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2810.5669437494956017No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2760.5568557824227262No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2730.5508030021790009No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2730.5508030021790009No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2620.5286094746186748No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2600.5245742877895246No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2570.5185215075457994No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2550.5144863207166492No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2470.4983455734000484No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2430.49027519974174805No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2410.48624001291259783No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2390.4822048260834477No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2390.4822048260834477No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2330.4700992655959971No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2270.4579937051085465No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2270.4579937051085465No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA2260.45597611169397145No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2160.4358001775482205No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2140.43176499071907026No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2130.42974739730449524No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2120.4277298038899201No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2090.4216770236461948No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA2030.40957146315874426No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1980.3994834960858688No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1970.39746590267129367No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1950.39343071584214345No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1900.383342748769268No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1890.3813251553546929No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1880.37930756194011783No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1870.3772899685255427No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1850.37325478169639253No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1810.36518440803809216No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1720.3470260673069163No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1690.340973287063191No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1590.32079735291744005No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1570.3167621660882899No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1570.3167621660882899No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1570.3167621660882899No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1550.31272697925913967No Hit
TGTGTTAAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1420.28649826486966345No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1400.2824630780405133No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1390.28044548462593816No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1360.27439270438221286No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1340.2703575175530627No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1310.2643047373093374No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1270.256234363651037No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1240.2501815834073118No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1200.24211120974901137No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1180.23807602291986119No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1170.23605842950528608No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1160.23404083609071102No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1160.23404083609071102No Hit
TGTGTTAAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1140.2300056492615608No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1090.21991768218868535No Hit
TGTGTTAAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1090.21991768218868535No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1080.21790008877411024No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1080.21790008877411024No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA1070.21588249535953513No Hit
TGTGTTAAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1070.21588249535953513No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1040.20982971511580983No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCAGGCGTTA1020.20579452828665967No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA1010.20377693487208456No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1000.2017593414575095No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC990.1997417480429344No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT970.1957065612137842No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG940.18965378097005892No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA930.18763618755548384No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT910.18360100072633362No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT880.17754822048260835No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG870.17553062706803324No Hit
TGTGTTAAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT870.17553062706803324No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCAGGCGTTA840.16947784682430797No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT820.16544265999515778No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA800.1614074731660076No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT790.15938987975143248No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA780.1573722863368574No Hit
TGTGTTAAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT780.1573722863368574No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA780.1573722863368574No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG750.1513195060931321No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT750.1513195060931321No Hit
TGTGTTAAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT750.1513195060931321No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC740.14930191267855702No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA740.14930191267855702No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT740.14930191267855702No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA730.14728431926398192No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA700.14123153902025665No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT690.13921394560568154No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG690.13921394560568154No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA680.13719635219110643No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC680.13719635219110643No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA660.13316116536195627No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT660.13316116536195627No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA660.13316116536195627No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA660.13316116536195627No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT660.13316116536195627No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC650.13114357194738116No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT640.12912597853280608No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT640.12912597853280608No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT630.12710838511823097No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA620.1250907917036559No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA620.1250907917036559No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA620.1250907917036559No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT610.1230731982890808No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT610.1230731982890808No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA610.1230731982890808No Hit
TGTGTTAAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT610.1230731982890808No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC600.12105560487450569No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA600.12105560487450569No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT590.11903801145993059No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC580.11702041804535551No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA570.1150028246307804No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT570.1150028246307804No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT560.11298523121620531No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC560.11298523121620531No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC530.10693245097248003No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA530.10693245097248003No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA530.10693245097248003No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA520.10491485755790492No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA510.10289726414332984No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT510.10289726414332984No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA510.10289726414332984No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC510.10289726414332984No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC510.10289726414332984No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA510.10289726414332984No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)