FastQCFastQC Report
Sun 21 May 2023
EGAF00007848030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61211
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA34355.6117364525983895No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA32915.3764846187776705No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA14142.310042312656222No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA13902.270833673686102No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA13842.261031513943572No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA13622.225090261554296No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT12462.0355818398653835No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT11881.9408276290209274No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT9511.553642319190995No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT9041.4768587345411772No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT8711.4229468559572627No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8521.391906683439251No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8491.3870056035679863No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8291.354331737759553No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA8261.349430657888288No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT8071.3183904853702766No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT7241.1827939422652791No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7231.1811602489748574No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7031.1484863831664245No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6641.0847723448399798No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6621.0815049582591365No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT6591.0766038783878713No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6531.0668017186453416No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT5920.9671464279296206No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5610.9165019359265493No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5540.9050660828935976No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA5310.8674911372138996No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5310.8674911372138996No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT5280.8625900573426345No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5050.8250151116629365No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5030.821747725082093No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT4870.7956086324353465No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4750.7760043129502867No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT4670.7629347666269134No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4530.7400630605610103No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4520.7384293672705886No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4230.6910522618483605No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4120.6730816356537224No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4010.6551110094590842No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT3840.627338223521916No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3800.6208034503602294No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3710.6061002107464345No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3650.5962980510039045No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT3620.5913969711326396No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3480.5685252650667364No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3410.5570894120337848No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3310.5407524791295683No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3300.5391187858391466No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3260.5325840126774599No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3200.52278185293493No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3200.52278185293493No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3190.5211481596445083No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT3000.4901079871264969No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2920.47703844080312363No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2820.460701507898907No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2480.40515593602457073No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2480.40515593602457073No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT2470.4035222427341491No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2430.39698746957246245No Hit
TGTGTTAAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT2420.3953537762820408No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2370.38718530982993254No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2310.3773831500874026No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2250.3675809903448726No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2190.3577788306023427No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2110.34470928427896946No Hit
TGTGTTAAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1990.3251049647939096No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1970.32183757821306624No Hit
TGTGTTAAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1950.31857019163222294No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1880.30713433859927136No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1880.30713433859927136No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1860.30386695201842806No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1860.30386695201842806No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1790.2924310989854765No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1790.2924310989854765No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1760.2875300191142115No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1740.2842626325333682No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1680.27446047279083824No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1650.2695593929195733No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1640.26792569962915164No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1610.26302461975788666No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1530.2499550734345134No Hit
TGTGTTAAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1530.2499550734345134No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1480.24178660698240514No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1390.2270833673686102No Hit
TGTGTTAAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1390.2270833673686102No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1350.2205485942069236No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1330.21728120762608027No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1300.21238012775481532No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1180.19277580826975543No Hit
TGTGTTAAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1160.18950842168891213No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1150.18787472839849045No Hit
TGTGTTAAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1140.1862410351080688No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1120.18297364852722547No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1100.1797062619463822No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1070.17480518207511722No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1050.1715377954942739No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1030.1682704089134306No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1020.16663671562300894No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1010.1650030223325873No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC1000.1633693290421656No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC990.16173563575174396No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT960.156834555880479No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA960.156834555880479No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA920.15029978271879238No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT920.15029978271879238No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA890.1453987028475274No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA880.14376500955710575No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA880.14376500955710575No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA880.14376500955710575No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT860.14049762297626245No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA850.13886392968584077No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT830.13559654310499747No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA820.13396284981457582No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT790.12906176994331084No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA790.12906176994331084No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA780.1274280766528892No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA780.1274280766528892No Hit
TGTGTTAAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT780.1274280766528892No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT760.12416069007204586No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA760.12416069007204586No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC750.12252699678162422No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA750.12252699678162422No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT750.12252699678162422No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC740.12089330349120257No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA740.12089330349120257No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA740.12089330349120257No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC730.11925961020078091No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT720.11762591691035924No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA720.11762591691035924No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA710.11599222361993759No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC710.11599222361993759No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT710.11599222361993759No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.11435853032951593No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA690.11272483703909428No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC680.11109114374867263No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC680.11109114374867263No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGAGGGCCCCAAGCGTTA680.11109114374867263No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT660.10782375716782931No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT660.10782375716782931No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT620.10128898400614268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)