FastQCFastQC Report
Sun 21 May 2023
EGAF00007848040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60781
Sequences flagged as poor quality0
Sequence length250
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG47797.862654447936032No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG45917.553347263124991No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG23293.83178953949425No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG21553.5455158684457313No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT11881.9545581678485053No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT10731.7653543047991973No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT9331.5350191671739524No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT8791.4461756140899296No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8671.42643260229348No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT8381.3787203237853933No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT8221.352396308056794No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7651.2586170020236587No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7521.2372287392441716No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7501.2339382372780967No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT7091.1664829469735607No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT5980.9838600878564024No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCACGCT5710.939438311314391No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5660.9312120563992037No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5330.8769187739589673No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5180.8522400092134055No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT5080.8357874993830309No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCT4980.8193349895526564No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCT4910.8078182326713941No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCT4730.7782037149767197No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4570.7518796992481203No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4200.6910054128757342No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4090.6729076520623222No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3810.6268406245372732No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3720.6120333656899359No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCACGCT3700.6087428637238611No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3550.5840640989782991No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3460.5692568401309619No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT3450.5676115891479245No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCT3420.5626758361988121No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCT3350.5511590793175498No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3310.5445780753854No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3290.5412875734193251No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3240.5330613185041377No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACGCT3210.5281255655550254No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3200.526480314571988No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3200.526480314571988No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCT3170.5215445616228755No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3080.5067373027755384No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2880.47383228311478914No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2860.4705417811487142No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2830.46560602819960184No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2820.46396077721656437No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2740.4507987693522647No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2690.4425725144370774No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2580.4244747536236653No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2560.4211842516575904No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2560.4211842516575904No Hit
TGTGTTAAGCCGGACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCT2460.4047317418272157No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2430.39979598887810336No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2430.39979598887810336No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2430.39979598887810336No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2350.38663398101380364No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2290.37676247511557887No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2240.36853622020039156No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCT2170.3570194633191293No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2140.35208371037001696No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2100.3455027064378671No Hit
TGTGTTAAGCCGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCT2080.3422122044717922No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2060.33892170250571724No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2050.33727645152267977No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1950.32082394169230516No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1900.31259768677711786No Hit
TGTGTTAAGCCGGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCT1900.31259768677711786No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1880.3093071848110429No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1830.3010809298958556No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1790.2944999259637058No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1690.27804741613333117No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1690.27804741613333117No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1670.2747569141672562No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1660.27311166318421876No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1610.2648854082690314No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1570.2583044043368816No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1530.25172340040473173No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1510.2484328984386568No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1500.24678764745561937No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1490.2451423964725819No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1480.24349714548954443No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1480.24349714548954443No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1480.24349714548954443No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1480.24349714548954443No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1460.24020664352346952No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1460.24020664352346952No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1450.23856139254043204No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1380.22704463565916982No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1380.22704463565916982No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1380.22704463565916982No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1350.2221088827100574No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1320.21717312976094502No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1320.21717312976094502No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1320.21717312976094502No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1310.21552787877790755No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1300.21388262779487008No Hit
TGTGTTAAGCCGGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCT1290.21223737681183266No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1280.2105921258287952No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1250.2056563728796828No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1230.20236587091360786No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1230.20236587091360786No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1230.20236587091360786No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1230.20236587091360786No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1220.2007206199305704No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1190.19578486698145803No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1170.19249436501538308No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1170.19249436501538308No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1160.19084911403234564No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1150.18920386304930817No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1140.1875586120662707No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1130.18591336108323325No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1100.18097760813412087No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1070.17604185518500848No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1030.16946085125285862No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA970.15958934535463384No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT960.15794409437159637No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA950.15629884338855893No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA950.15629884338855893No Hit
TGTGTTAAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT950.15629884338855893No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA940.15465359240552146No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA930.15300834142248398No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA910.14971783945640907No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC900.14807258847337162No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC890.14642733749033415No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC860.14149158454122177No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT850.13984633355818432No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT820.1349105806090719No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT810.13326532962603443No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC790.12997482765995952No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT790.12997482765995952No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC780.12832957667692207No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA770.1266843256938846No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT770.1266843256938846No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC760.12503907471084713No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA760.12503907471084713No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC750.12339382372780969No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA750.12339382372780969No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT750.12339382372780969No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC740.12174857274477222No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT730.12010332176173476No Hit
TGTGTTAAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT730.12010332176173476No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC720.11845807077869729No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA710.11681281979565983No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC690.11352231782958491No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC690.11352231782958491No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT680.11187706684654744No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA680.11187706684654744No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA660.10858656488047251No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC660.10858656488047251No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT630.10365081193136014No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA630.10365081193136014No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT630.10365081193136014No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA630.10365081193136014No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.10200556094832267No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC610.1003603099652852No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC610.1003603099652852No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA610.1003603099652852No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)