FastQCFastQC Report
Sun 21 May 2023
EGAF00007848041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60781
Sequences flagged as poor quality0
Sequence length250
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG42897.056481466247676No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG41386.808048567809019No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG21893.601454401869005No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG20163.3168259818035244No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT12642.0795972425593523No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT12282.0203682071700038No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT9991.6436057320544248No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT9691.5942482025633011No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT8801.447820865072967No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT8741.437949359174742No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8251.3573320610059063No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7771.2783600138201083No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT6571.0809298958556126No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6551.0776393938895379No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT6491.067767887991313No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6491.067767887991313No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT6081.000312597686777No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCACGCT5880.967407578026028No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCT5870.9657623270429905No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5730.9427288132804659No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCT5220.8588210131455554No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCT5010.8242707425017686No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4470.7354271894177457No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4400.7239104325364835No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCACGCT4110.676198154028397No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACGCT3940.6482288873167602No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT3930.6465836363337227No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3930.6465836363337227No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3780.6219048715881608No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3760.6186143696220858No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3740.6153238676560109No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3700.6087428637238611No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3690.6070976127408236No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCT3660.6021618597917112No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3600.5922903538934865No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3600.5922903538934865No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3570.5873546009443741No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3540.5824188479952617No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3480.5725473420970368No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3350.5511590793175498No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCT3290.5412875734193251No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3240.5330613185041377No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3050.501801549826426No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3010.49522054589427617No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2810.46231552623352695No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2780.4573797732844146No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2710.4458630164031523No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2690.4425725144370774No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2650.43599151050492757No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2620.4310557575558151No Hit
TGTGTTAAGCCGGACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCT2600.42776525558974016No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2590.42612000460670274No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2490.4096674947763282No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCT2480.40802224379329066No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2430.39979598887810336No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2430.39979598887810336No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCT2410.3965054869120284No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2350.38663398101380364No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2350.38663398101380364No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2240.36853622020039156No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2200.36195521626824173No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2180.3586647143021668No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2140.35208371037001696No Hit
TGTGTTAAGCCGGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCT2120.348793208403942No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2080.3422122044717922No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2080.3422122044717922No Hit
TGTGTTAAGCCGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCT2050.33727645152267977No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1930.3175334397262302No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1910.3142429377601553No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1900.31259768677711786No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1900.31259768677711786No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1870.30766193382800544No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1860.30601668284496797No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1830.3010809298958556No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1780.2928546749806683No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1770.29120942399763083No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1720.28298316908244353No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1720.28298316908244353No Hit
TGTGTTAAGCCGGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCT1690.27804741613333117No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1670.2747569141672562No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1660.27311166318421876No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1650.2714664122011813No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1610.2648854082690314No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1550.2550139023708067No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1530.25172340040473173No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1510.2484328984386568No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1500.24678764745561937No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1500.24678764745561937No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1470.241851894506507No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1440.23691614155739457No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1440.23691614155739457No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1430.2352708905743571No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1370.22539938467613235No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1330.2188183807439825No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1280.2105921258287952No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1270.20894687484575772No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1250.2056563728796828No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1230.20236587091360786No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1220.2007206199305704No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1220.2007206199305704No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1220.2007206199305704No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1190.19578486698145803No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1180.19413961599842056No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1180.19413961599842056No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1160.19084911403234564No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1150.18920386304930817No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1130.18591336108323325No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1110.18262285911715834No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1110.18262285911715834No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1090.1793323571510834No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1090.1793323571510834No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1080.17768710616804592No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1070.17604185518500848No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1060.174396604201971No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1020.16781560026982115No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT980.16123459633767132No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA970.15958934535463384No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA970.15958934535463384No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT970.15958934535463384No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC960.15794409437159637No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT960.15794409437159637No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT940.15465359240552146No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT890.14642733749033415No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC870.1431368355242592No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA840.13820108257514685No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT840.13820108257514685No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA830.13655583159210938No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT800.131620078642997No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC790.12997482765995952No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC780.12832957667692207No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT780.12832957667692207No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC780.12832957667692207No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA770.1266843256938846No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT770.1266843256938846No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA760.12503907471084713No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT760.12503907471084713No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA760.12503907471084713No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT750.12339382372780969No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC750.12339382372780969No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT750.12339382372780969No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT720.11845807077869729No Hit
TGTGTTAAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT710.11681281979565983No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA710.11681281979565983No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA710.11681281979565983No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT700.11516756881262237No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA690.11352231782958491No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC670.11023181586350998No Hit
TGTGTTAAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT670.11023181586350998No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT670.11023181586350998No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC660.10858656488047251No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC660.10858656488047251No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.1052960629143976No Hit
TGTGTTAAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT640.1052960629143976No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT630.10365081193136014No Hit
TGTGTTAAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT620.10200556094832267No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA610.1003603099652852No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)