FastQCFastQC Report
Sun 21 May 2023
EGAF00007848044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21962
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA14486.593206447500227No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10234.658045715326473No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8523.8794281030871507No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7353.3466897368181403No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6132.7911847737000275No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5812.645478553865768No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4672.12640014570622No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4552.0717603132683724No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4241.9306074128039339No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4021.830434386667881No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3711.6892814862034422No Hit
AATCTGACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3421.557235224478645No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2881.3113559785083324No Hit
AATCTGACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2861.3022493397686914No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2711.2339495492213823No Hit
AATCTGACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2461.1201165649758675No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2411.0973499681267644No Hit
AATCTGACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2321.056370093798379No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2100.9561970676623258No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2030.9243238320735817No Hit
AATCTGACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1980.9015572352244786No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1970.8970039158546581No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1870.851470722156452No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1870.851470722156452No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1720.783170931609143No Hit
AATCTGACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1670.76040433476004No Hit
AATCTGACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1640.7467443766505784No Hit
AATCTGACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1630.7421910572807577No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1590.7239777798014753No Hit
AATCTGACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1560.7103178216920134No Hit
AATCTGACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1540.7012111829523723No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1510.6875512248429105No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1480.6738912667334487No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1400.637464711774884No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1380.6283580730352426No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1370.6238047536654221No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1340.6101447955559604No Hit
AATCTGACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1220.5555049631181131No Hit
AATCTGACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1200.5463983243784719No Hit
AATCTGACCCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC1170.5327383662690102No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1160.5281850468991895No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1150.5236317275293689No Hit
AATCTGACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1070.4872051725708041No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1070.4872051725708041No Hit
AATCTGACCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC1070.4872051725708041No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1060.48265185320098347No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1060.48265185320098347No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC1050.4780985338311629No Hit
AATCTGACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1040.4735452144613423No Hit
AATCTGACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1010.4598852563518805No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT970.4416719788725982No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA950.4325653401329569No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA890.4052454239140333No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA850.3870321464347509No Hit
AATCTGACCCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC810.3688188689554685No Hit
AATCTGACCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC800.36426554958564794No Hit
AATCTGACCCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC800.36426554958564794No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT780.3551589108460067No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA780.3551589108460067No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA770.35060559147618614No Hit
AATCTGACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT750.3414989527365449No Hit
AATCTGACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT730.3323923139969038No Hit
AATCTGACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT720.3278389946270831No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC710.32328567525726254No Hit
AATCTGACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT690.3141790365176213No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG690.3141790365176213No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA660.30051907840815956No Hit
AATCTGACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT660.30051907840815956No Hit
AATCTGACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT660.30051907840815956No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA660.30051907840815956No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA630.28685912029869776No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT630.28685912029869776No Hit
AATCTGACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT630.28685912029869776No Hit
AATCTGACCCCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACC630.28685912029869776No Hit
AATCTGACCCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC610.2777524815590566No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA590.26864584281941534No Hit
AATCTGACCCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC590.26864584281941534No Hit
AATCTGACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT590.26864584281941534No Hit
AATCTGACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT570.25953920407977416No Hit
AATCTGACCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT560.25498588470995354No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC560.25498588470995354No Hit
AATCTGACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT550.250432565340133No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC550.250432565340133No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG550.250432565340133No Hit
AATCTGACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT550.250432565340133No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.24587924597031235No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG510.23221928786085055No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG510.23221928786085055No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT490.22311264912120934No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG490.22311264912120934No Hit
AATCTGACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT480.21855932975138875No Hit
AATCTGACCCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACC480.21855932975138875No Hit
AATCTGACCCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACC470.2140060103815682No Hit
AATCTGACCCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACC460.20945269101174754No Hit
AATCTGACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT450.20489937164192695No Hit
AATCTGACCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC440.2003460522721064No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC420.19123941353246515No Hit
AATCTGACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT390.17757945542300335No Hit
AATCTGACCCCGGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACC390.17757945542300335No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG380.1730261360531828No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA380.1730261360531828No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA370.16847281668336217No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA370.16847281668336217No Hit
AATCTGACCCCGGACTACGAGGGTATCTAATCCTGTTTGATACCCGCACC370.16847281668336217No Hit
AATCTGACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT370.16847281668336217No Hit
AATCTGACCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC370.16847281668336217No Hit
AATCTGACCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC360.16391949731354155No Hit
AATCTGACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT340.15481285857390037No Hit
AATCTGACCCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT330.15025953920407978No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG330.15025953920407978No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA330.15025953920407978No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA320.1457062198342592No Hit
AATCTGACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT320.1457062198342592No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA320.1457062198342592No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA320.1457062198342592No Hit
AATCTGACCCCGGACTACAGGGGTATCTAATCCTGTTTGATACCCGCACC310.14115290046443857No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA310.14115290046443857No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG300.13659958109461798No Hit
AATCTGACCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT290.1320462617247974No Hit
AATCTGACCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC290.1320462617247974No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA290.1320462617247974No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT280.12749294235497677No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA280.12749294235497677No Hit
AATCTGACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT280.12749294235497677No Hit
AATCTGACCCCGGACTACCCGGGTATCTAATCCTGTTTGATACCCGCACC280.12749294235497677No Hit
AATCTGACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT270.12293962298515618No Hit
AATCTGACCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC270.12293962298515618No Hit
AATCTGACCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT260.11838630361533557No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT260.11838630361533557No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA260.11838630361533557No Hit
AATCTGACCCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT250.113832984245515No Hit
AATCTGACCCCGGACTACAAGGGTATCTAATCCTGTTTGATACCCGCACC250.113832984245515No Hit
AATCTGACCCCGGACTACACGGGTATCTAATCCTGTTTGATACCCGCACC250.113832984245515No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA240.10927966487569438No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT240.10927966487569438No Hit
AATCTGACCCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT240.10927966487569438No Hit
AATCTGACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT230.10472634550587377No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA230.10472634550587377No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA230.10472634550587377No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT220.1001730261360532No Hit
AATCTGACCCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC220.1001730261360532No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA220.1001730261360532No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA220.1001730261360532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)