FastQCFastQC Report
Sun 21 May 2023
EGAF00007848045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21962
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13276.042254803751935No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10014.55787268919042No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7633.474182679173117No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7163.260176668791549No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5552.5270922502504325No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5492.499772334031509No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5342.4314725434842No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4772.171933339404426No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4191.907840815954831No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3761.7120480830525455No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3461.5754485019579274No Hit
AATCTGACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3211.4616155177124124No Hit
AATCTGACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3131.4251889627538477No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3121.4206356433840268No Hit
AATCTGACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2711.2339495492213823No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2561.1656497586740735No Hit
AATCTGACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2431.1064566068664057No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC2321.056370093798379No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2231.0153902194699935No Hit
AATCTGACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2050.9334304708132228No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2040.9288771514434022No Hit
AATCTGACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2010.9152171933339405No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1980.9015572352244786No Hit
AATCTGACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1840.8378107640469902No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1800.8195974865677078No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1670.76040433476004No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1600.7285310991712959No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1590.7239777798014753No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC1540.7012111829523723No Hit
AATCTGACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1520.6921045442127312No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1470.6693379473636281No Hit
AATCTGACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1470.6693379473636281No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1450.6602313086239869No Hit
AATCTGACCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC1360.6192514342956015No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1340.6101447955559604No Hit
AATCTGACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1310.5964848374464985No Hit
AATCTGACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1270.5782715599672161No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1260.5737182405973955No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1250.5691649212275749No Hit
AATCTGACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1230.5600582824879338No Hit
AATCTGACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1220.5555049631181131No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1220.5555049631181131No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1200.5463983243784719No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1140.5190784081595483No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1120.5099717694199071No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1100.500865130680266No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1090.49631181131044527No Hit
AATCTGACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1080.4917584919406247No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1050.4780985338311629No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1010.4598852563518805No Hit
AATCTGACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1000.45533193698206No Hit
AATCTGACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT980.4462252982424187No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA940.4280120207631364No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA910.4143520626536746No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA890.4052454239140333No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA880.4006921045442128No Hit
AATCTGACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT870.3961387851743921No Hit
AATCTGACCCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC850.3870321464347509No Hit
AATCTGACCCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC810.3688188689554685No Hit
AATCTGACCCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC780.3551589108460067No Hit
AATCTGACCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT760.3460522721063656No Hit
AATCTGACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT760.3460522721063656No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA760.3460522721063656No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT740.33694563336672434No Hit
AATCTGACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT740.33694563336672434No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC730.3323923139969038No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG710.32328567525726254No Hit
AATCTGACCCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC700.318732355887442No Hit
AATCTGACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT690.3141790365176213No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA670.3050723977779802No Hit
AATCTGACCCCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACC670.3050723977779802No Hit
AATCTGACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT660.30051907840815956No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG650.29596575903833894No Hit
AATCTGACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT640.2914124396685184No Hit
AATCTGACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT620.28230580092887714No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC620.28230580092887714No Hit
AATCTGACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT600.27319916218923596No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.26864584281941534No Hit
AATCTGACCCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC590.26864584281941534No Hit
AATCTGACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT580.2640925234495948No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA570.25953920407977416No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA550.250432565340133No Hit
AATCTGACCCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACC550.250432565340133No Hit
AATCTGACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT540.24587924597031235No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG540.24587924597031235No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC530.24132592660049174No Hit
AATCTGACCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC530.24132592660049174No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA520.23677260723067114No Hit
AATCTGACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT500.22766596849103No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC500.22766596849103No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT490.22311264912120934No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG480.21855932975138875No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT470.2140060103815682No Hit
AATCTGACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT460.20945269101174754No Hit
AATCTGACCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC450.20489937164192695No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT430.19579273290228574No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG420.19123941353246515No Hit
AATCTGACCCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT420.19123941353246515No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA410.1866860941626446No Hit
AATCTGACCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC410.1866860941626446No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC410.1866860941626446No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA400.18213277479282397No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA400.18213277479282397No Hit
AATCTGACCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT390.17757945542300335No Hit
AATCTGACCCCGGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACC390.17757945542300335No Hit
AATCTGACCCCGGACTACGAGGGTATCTAATCCTGTTTGATACCCGCACC380.1730261360531828No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA380.1730261360531828No Hit
AATCTGACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT380.1730261360531828No Hit
AATCTGACCCCGGACTACAGGGGTATCTAATCCTGTTTGATACCCGCACC370.16847281668336217No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA370.16847281668336217No Hit
AATCTGACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT370.16847281668336217No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG340.15481285857390037No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG340.15481285857390037No Hit
AATCTGACCCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT340.15481285857390037No Hit
AATCTGACCCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT340.15481285857390037No Hit
AATCTGACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT330.15025953920407978No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA330.15025953920407978No Hit
AATCTGACCCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACC320.1457062198342592No Hit
AATCTGACCCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT310.14115290046443857No Hit
AATCTGACCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC310.14115290046443857No Hit
AATCTGACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT310.14115290046443857No Hit
AATCTGACCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC300.13659958109461798No Hit
AATCTGACCCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT300.13659958109461798No Hit
AATCTGACCCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC300.13659958109461798No Hit
AATCTGACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT270.12293962298515618No Hit
AATCTGACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT270.12293962298515618No Hit
AATCTGACCCCGGACTACACGGGTATCTAATCCTGTTTGATACCCGCACC270.12293962298515618No Hit
AATCTGACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT270.12293962298515618No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA260.11838630361533557No Hit
AATCTGACCCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACC260.11838630361533557No Hit
AATCTGACCCCGGACTACAAGGGTATCTAATCCTGTTTGATACCCGCACC260.11838630361533557No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA260.11838630361533557No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA260.11838630361533557No Hit
AATCTGACCCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT250.113832984245515No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT250.113832984245515No Hit
AATCTGACCCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC250.113832984245515No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCC250.113832984245515No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA250.113832984245515No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA250.113832984245515No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA240.10927966487569438No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA240.10927966487569438No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT230.10472634550587377No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA230.10472634550587377No Hit
AATCTGACCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC230.10472634550587377No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA220.1001730261360532No Hit
AATCTGACCCCGGACTACCAGGGTATCTAATCCTGTTTGATACCCGCACC220.1001730261360532No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG220.1001730261360532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)