FastQCFastQC Report
Sun 21 May 2023
EGAF00007848052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47116
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA17203.6505645640546738No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA16043.4043636981068No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG11252.387723915442737No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG11002.334663383988454No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9862.0927073605569233No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9181.9483827150012734No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7501.5918159436284913No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7431.576958994821292No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7131.5132863570761526No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6911.4665930893963834No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6641.4092877154257577No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6211.318023601324391No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5981.2692079123864504No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5851.2416164360302233No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5701.2097801171576534No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5411.148229900670685No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5351.1354953731216573No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4961.0527209440529757No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4921.0442312590202902No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4741.0060276763732066No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4530.9614568299516087No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4360.9253756685626964No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4160.8829272433992699No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4160.8829272433992699No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4070.863825452075728No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3960.8404788182358434No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3890.8256218694286441No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3790.804397656846931No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3640.7725613379743612No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3620.7683164954580185No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA3510.7449698616181339No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3500.7428474403599626No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3420.7258680702945921No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3340.7088887002292216No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3240.6876644876475083No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3060.6494609050004245No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA3050.6473384837422532No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3030.6430936412259105No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3010.6388487987095679No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2930.6218694286441973No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2920.619747007386026No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2910.6176245861278546No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2900.6155021648696833No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2870.6091349010951694No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2830.600645216062484No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2810.5964003735461415No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA2630.5581967908990576No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2630.5581967908990576No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2590.5497071058663723No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2570.5454622633500298No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2490.5284828932846591No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2440.5178707869938025No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2420.5136259444774599No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2400.5093811019611172No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2390.5072586807029459No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2320.49240173189574665No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2270.48178962560489No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2180.4626878342813482No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2080.44146362169963493No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2010.4266066728924357No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1950.4138721453434077No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1950.4138721453434077No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1950.4138721453434077No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1930.40962730282706517No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1910.4053824603107225No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1900.4032600390525511No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1870.3968927752780372No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1710.36293403514729605No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1700.36081161388912475No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1690.3586891926309534No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1660.3523219288564394No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1570.33322013753289753No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1510.3204856099838696No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1510.3204856099838696No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1510.3204856099838696No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1500.3183631887256983No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1480.31411834620935564No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1480.31411834620935564No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1450.30775108243484167No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1440.3056286611766703No Hit
TGTGTTAAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1420.3013838186603277No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1390.29501655488581374No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1320.28015960607861445No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1290.27379234230410054No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1270.2695474997877578No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC1250.2653026572714152No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1240.2631802360132439No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC1170.24832328720604466No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1140.2419560234315307No Hit
TGTGTTAAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1130.23983360217335936No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1110.2355887596570167No Hit
TGTGTTAAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1110.2355887596570167No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1110.2355887596570167No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1100.23346633839884542No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1090.2313439171406741No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1070.22709907462433143No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1060.22497665336616013No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1050.2228542321079888No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1020.21648696833347483No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1010.2143645470753035No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC1000.21224212581713217No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT1000.21224212581713217No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1000.21224212581713217No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1000.21224212581713217No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA990.21011970455896084No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA980.20799728330078956No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA980.20799728330078956No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT950.20163001952627554No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA930.19738517700993294No Hit
TGTGTTAAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT930.19738517700993294No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT920.1952627557517616No Hit
TGTGTTAAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT900.19101791323541895No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT880.1867730707190763No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA870.184650649460905No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT870.184650649460905No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC850.18040580694456237No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG840.17828338568639102No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG840.17828338568639102No Hit
TGTGTTAAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT830.1761609644282197No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA810.17191612191187708No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT800.16979370065370575No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA800.16979370065370575No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA800.16979370065370575No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA790.16767127939553442No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC780.1655488581373631No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT750.15918159436284915No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT730.1549367518465065No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA730.1549367518465065No Hit
TGTGTTAAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT720.15281433058833516No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC710.15069190933016385No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT680.1443246455556499No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC670.14220222429747856No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.14220222429747856No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.1358349605229646No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA640.1358349605229646No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA620.13159011800662196No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.13159011800662196No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.13159011800662196No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT620.13159011800662196No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACT610.12946769674845063No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTCGATACCCGCACC590.125222854232108No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA590.125222854232108No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC580.12310043297393666No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT580.12310043297393666No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.12310043297393666No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.11885559045759402No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.11885559045759402No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT550.11673316919942271No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT550.11673316919942271No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA550.11673316919942271No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT540.11461074794125138No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT540.11461074794125138No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT540.11461074794125138No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC530.11248832668308006No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCT520.11036590542490873No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT520.11036590542490873No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA520.11036590542490873No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA520.11036590542490873No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT510.10824348416673742No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT500.10612106290856609No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.10612106290856609No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT490.10399864165039478No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT480.10187622039222344No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT480.10187622039222344No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA480.10187622039222344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)