FastQCFastQC Report
Sun 21 May 2023
EGAF00007848092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29552
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10243.4650785056848945No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9613.2518949648077964No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9273.1368435300487274No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7972.6969409853817No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5441.8408229561451002No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4921.6648619382782892No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4551.5396589063345967No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4441.5024363833243097No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4351.4719815917704386No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4231.4313752030319438No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4151.4043042772062804No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3981.346778559826746No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3741.2655657823497564No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3701.2520303194369249No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3661.2384948565240932No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3311.1200595560368165No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3261.1031402273957769No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3151.06591770438549No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3061.0354629128316188No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3011.0185435841905792No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3001.0151597184623713No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2961.0016242555495398No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2930.991472658364916No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2890.9779371954520845No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2730.923795343800758No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2670.9034921494315106No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2530.8561180292366No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2450.8290471034109367No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2260.7647536545749865No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2260.7647536545749865No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2220.7512181916621549No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2170.7342988630211154No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2110.7139956686518679No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2080.7038440714672441No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1990.673389279913373No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1970.6666215484569572No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1940.6564699512723335No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1890.639550622631294No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1890.639550622631294No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1870.6327828911748782No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1820.6158635625338387No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1690.5718733080671359No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1630.5515701136978884No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1630.5515701136978884No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1610.5448023822414726No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1580.5346507850568489No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1540.5211153221440173No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1510.5109637249593936No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1500.5075798592311856No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1470.497428262046562No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1470.497428262046562No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1420.4805089334055225No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1360.46020573903627504No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1320.44667027612344345No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1280.4331348132106118No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1230.4162154845695723No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1210.4094477531131565No Hit
TGTGTTAAGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1200.4060638873849486No Hit
TGTGTTAAGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1180.3992961559285328No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1180.3992961559285328No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1150.389144558743909No Hit
TGTGTTAAGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1050.35530590146183No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1040.3519220357336221No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA990.33500270709258256No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT990.33500270709258256No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA970.32823497563616677No Hit
TGTGTTAAGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT970.32823497563616677No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA960.32485110990795885No Hit
TGTGTTAAGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT940.31808337845154305No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT940.31808337845154305No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA940.31808337845154305No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC910.30793178126691934No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA910.30793178126691934No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA890.30116404981050354No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA890.30116404981050354No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA850.2876285868976719No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA830.28086085544125605No Hit
TGTGTTAAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT820.2774769897130482No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA820.2774769897130482No Hit
TGTGTTAAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT790.26732539252842447No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA770.2605576610720087No Hit
TGTGTTAAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT760.25717379534380075No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC720.24363833243096913No Hit
TGTGTTAAGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT690.23348673524634542No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA690.23348673524634542No Hit
TGTGTTAAGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT690.23348673524634542No Hit
TGTGTTAAGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT690.23348673524634542No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA680.23010286951813752No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.2165674066053059No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.2165674066053059No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT630.213183540877098No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA610.2064158094206822No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC610.2064158094206822No Hit
TGTGTTAAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT610.2064158094206822No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA600.2030319436924743No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC600.2030319436924743No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA590.1996480779642664No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA590.1996480779642664No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA580.1962642122360585No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT570.19288034650785058No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT570.19288034650785058No Hit
TGTGTTAAGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT550.18611261505143475No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA550.18611261505143475No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC540.18272874932322686No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.18272874932322686No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA540.18272874932322686No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA520.17596101786681104No Hit
TGTGTTAAGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC500.16919328641039524No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC500.16919328641039524No Hit
TGTGTTAAGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.16919328641039524No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA490.16580942068218735No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA490.16580942068218735No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT490.16580942068218735No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT480.16242555495397942No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA480.16242555495397942No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTG470.15904168922577153No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA470.15904168922577153No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT470.15904168922577153No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA470.15904168922577153No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT460.15565782349756363No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA450.15227395776935573No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTG440.1488900920411478No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTG440.1488900920411478No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA430.14550622631293988No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA430.14550622631293988No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT430.14550622631293988No Hit
TGTGTTAAGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT430.14550622631293988No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA430.14550622631293988No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT420.142122360584732No Hit
TGTGTTAAGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT420.142122360584732No Hit
TGTGTTAAGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT410.1387384948565241No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA410.1387384948565241No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA410.1387384948565241No Hit
TGTGTTAAGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC410.1387384948565241No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA400.1353546291283162No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT400.1353546291283162No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT400.1353546291283162No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC400.1353546291283162No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA400.1353546291283162No Hit
TGTGTTAAGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC400.1353546291283162No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT390.13197076340010827No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC380.12858689767190037No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA380.12858689767190037No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA370.12520303194369248No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA370.12520303194369248No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC370.12520303194369248No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA360.12181916621548457No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA360.12181916621548457No Hit
TGTGTTAAGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT350.11843530048727667No Hit
TGTGTTAAGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT350.11843530048727667No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT350.11843530048727667No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG340.11505143475906876No Hit
TGTGTTAAGCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT330.11166756903086086No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA310.10489983757444506No Hit
TGTGTTAAGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT310.10489983757444506No Hit
TGTGTTAAGCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT310.10489983757444506No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA310.10489983757444506No Hit
TGTGTTAAGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC310.10489983757444506No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA310.10489983757444506No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTG300.10151597184623715No Hit
TGTGTTAAGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC300.10151597184623715No Hit
TGTGTTAAGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT300.10151597184623715No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)