FastQCFastQC Report
Sun 21 May 2023
EGAF00007848097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70901
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA60148.482249897744742No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA32534.588087615125316No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA22183.128305665646465No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTA19302.7221054710088715No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCC13481.9012425776787352No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12411.7503279220321293No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCC12161.7150674884698383No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA11591.6346736999478146No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCC10261.447088193396426No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTA9971.4061860904641683No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCC9701.368104822216894No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9261.3060464591472616No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCC8861.2496297654475959No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCC8611.2143693318853048No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA8171.1523109688156725No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7191.0140900692514916No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6850.9661358796067757No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6530.9210025246470431No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCC6450.90971918590711No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTA6430.9068983512221266No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCC6020.8490712401799692No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5980.8434295708100026No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5940.837787901440036No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5910.8335566494125611No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCC5740.8095795545902033No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCC5670.7997066331927618No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCC5490.7743191210279121No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5010.7066190885883132No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA4950.6981565845333634No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCC4840.6826419937659554No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4820.6798211590809721No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4700.6628961509710724No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT4500.6346878041212395No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4410.6219940480388148No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4220.5951961185314735No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4070.5740398583940989No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTA4040.5698086063666239No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4020.5669877716816406No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCC3970.5599356849691823No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT3950.557114850284199No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3940.5557044329417075No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3860.5444210942017743No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCACGCC3770.5317273381193495No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3730.526085668749383No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3700.521854416721908No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3690.5204439993794164No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT3680.5190335820369247No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCC3570.5035189912695167No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCC3410.48095231378965037No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA3410.48095231378965037No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3350.47248980973470056No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGATACCCACGCC3340.47107939239220886No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACT3270.46120647099476736No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACT3270.46120647099476736No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3250.4583856363097841No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3210.4527439669398175No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3010.4245356200899846No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3010.4245356200899846No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2990.4217147854050013No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACT2950.4160731160350348No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2870.4047897772951016No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGATACCCACGCC2850.4019689426101184No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2760.38927518652769355No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2530.35683558765038575No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2510.35401475296540247No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2480.34978350093792754No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACT2460.34696266625294425No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2460.34696266625294425No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGATACCCACGCC2440.34414183156796097No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2300.32439598877307796No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2280.3215751540880947No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2240.3159334847181281No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACT2230.31452306737563646No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2040.28772513786829523No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2030.2863147205258036No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2020.28490430318331195No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGATACCCACGCC1990.280673051155837No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1970.27785221647085373No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1970.27785221647085373No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1950.27503138178587044No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGATACCCACGCC1940.2736209644433788No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1940.2736209644433788No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1910.26938971241590387No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACT1900.2679792950734122No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACACT1900.2679792950734122No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1870.2637480430459373No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1850.260927208360954No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1820.2566959563334791No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1820.2566959563334791No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1800.2538751216484958No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACT1800.2538751216484958No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACACT1800.2538751216484958No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGATACCCACGCC1750.24682303493603758No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1740.24541261759354593No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1710.24118136556607098No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGATACCCACGCC1660.2341292788536128No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1600.22566677479866293No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1600.22566677479866293No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1570.221435522771188No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1540.21720427074371304No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1500.2115626013737465No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1500.2115626013737465No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACACT1470.2073313493462716No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACT1410.1988688452913217No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1360.1918167585788635No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1290.18194383718142199No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1280.18053341983893034No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1230.17348133312647213No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1230.17348133312647213No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1230.17348133312647213No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACACT1220.17207091578398048No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1210.17066049844148884No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1180.1664292464140139No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1170.16501882907152227No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1170.16501882907152227No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1120.15796674235906405No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTC1100.15514590767408076No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1090.15373549033158912No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1080.15232507298909748No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1050.14809382096162255No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1030.14527298627663926No Hit
TACACATGACCGGACTACAAGGGTATCTAATCCTGTTTGATACCCACGCC1030.14527298627663926No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACACT1030.14527298627663926No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1030.14527298627663926No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1010.14245215159165597No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1000.14104173424916433No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGATACCCACACT990.1396313169066727No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC980.13822089956418104No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA970.1368104822216894No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT950.1339896475367061No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG950.1339896475367061No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT920.12975839550923118No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGATACCCACACT910.12834797816673954No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG900.1269375608242479No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT870.12270630879677297No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTC840.11847505676929804No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT830.1170646394268064No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGATACCCACACT830.1170646394268064No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT820.11565422208431475No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC820.11565422208431475No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA820.11565422208431475No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC800.11283338739933146No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT790.11142297005683982No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGATACCCACACT780.11001255271434818No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA780.11001255271434818No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC770.10860213537185652No Hit
TACACATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT770.10860213537185652No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT770.10860213537185652No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC770.10860213537185652No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.10719171802936489No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC750.10578130068687325No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA750.10578130068687325No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT750.10578130068687325No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA730.10296046600188995No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAACACGTAAGGTGCGAGCGTTA730.10296046600188995No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG710.10013963131690667No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC710.10013963131690667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)