FastQCFastQC Report
Sun 21 May 2023
EGAF00007848115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43377
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA17253.9767618784148278No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA12062.7802752610830628No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10052.3168960509025522No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9022.0794430228001013No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8631.989533623809853No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG8291.9111510708439958No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6931.5976208589805658No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6791.5653456901122715No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6451.486963137146414No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6271.445466491458607No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5981.3786107845171405No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5951.371694676902506No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5911.362473200082993No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5861.3509463540586024No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5781.3325034004195773No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5321.226456416995182No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4971.1457684948244462No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4891.1273255411854208No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4621.0650805726537105No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4561.0512483574244416No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4471.0305000345805382No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4401.0143624501463908No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4250.9797819120732185No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4210.9705604352537058No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4190.9659496968439495No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4160.9590335892293151No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4070.9382852663854117No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4000.9221476819512644No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3800.8760402978537012No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3780.871429559443945No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3740.8622080826244324No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3730.8599027134195543No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3550.8184060677317473No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3130.7215805611268644No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3050.7031376074878392No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3040.700832238282961No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2960.6823892846439358No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2910.670862438619545No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2750.6339765313414943No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2640.6086174700878345No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2640.6086174700878345No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2630.6063121008829564No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2540.5855637780390529No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2460.5671208244000276No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2460.5671208244000276No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2340.5394563939414897No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2230.51409733268783No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2200.5071812250731954No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2030.46798994859026677No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2030.46798994859026677No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1970.4541577333609978No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1950.4495469949512414No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1890.4357147797219725No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1810.41727182608294716No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1780.4103557184683127No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1770.4080503492634346No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1700.3919127648292874No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1690.38960739562440927No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1620.3734698111902621No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1580.3642483343707495No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1560.35963759596099315No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1550.357332226756115No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1540.3550268575512368No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1530.3527214883463587No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1500.3458053807317242No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1490.343500011526846No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1480.3411946423219679No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1310.30200336583903914No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1300.29969799663416097No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1290.2973926274292828No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1290.2973926274292828No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1290.2973926274292828No Hit
TGTGCCTGTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1260.29047651981464834No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1250.28817115060977017No Hit
TGTGCCTGTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1230.2835604122000138No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1220.28125504299513565No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1210.27894967379025754No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1150.2651174585609885No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1150.2651174585609885No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1150.2651174585609885No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1140.2628120893561104No Hit
TGTGCCTGTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1140.2628120893561104No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1110.25589598174147593No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1100.2535906125365977No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1090.2512852433317196No Hit
TGTGCCTGTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1080.2489798741268414No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1070.24667450492196324No Hit
TGTGCCTGTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1050.24206376651220693No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1040.23975839730732879No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1030.2374530281024506No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1030.2374530281024506No Hit
TGTGCCTGTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1030.2374530281024506No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1020.23514765889757244No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1020.23514765889757244No Hit
TGTGCCTGTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT990.22823155128293798No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA990.22823155128293798No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA970.22362081287318164No Hit
TGTGCCTGTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT960.2213154436683035No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA940.21670470525854715No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA930.21439933605366898No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC930.21439933605366898No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC930.21439933605366898No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC890.20517785923415635No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC870.20056712082440004No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA860.1982617516195219No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC840.19365101320976555No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA840.19365101320976555No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA830.19134564400488738No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC820.18904027480000923No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.18673490559513106No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA790.18212416718537475No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA770.1775134287756184No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC750.1729026903658621No Hit
TGTGCCTGTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT740.17059732116098394No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC740.17059732116098394No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC740.17059732116098394No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA730.16829195195610577No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT710.16368121354634946No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA710.16368121354634946No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA710.16368121354634946No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC700.1613758443414713No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA700.1613758443414713No Hit
TGTGCCTGTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT700.1613758443414713No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT690.15907047513659314No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT650.14984899831708048No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC650.14984899831708048No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT640.14754362911220234No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT630.14523825990732417No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC620.142932890702446No Hit
TGTGCCTGTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT610.14062752149756783No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC610.14062752149756783No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA610.14062752149756783No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACC600.13832215229268968No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT570.1314060446780552No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT560.12910067547317702No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA560.12910067547317702No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA550.12679530626829885No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT550.12679530626829885No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.1244899370634207No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.12218456785854255No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.11987919865366439No Hit
TGTGCCTGTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT510.11757382944878622No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT510.11757382944878622No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAACACGTAAGGTGCGAGCGTTG510.11757382944878622No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT510.11757382944878622No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA510.11757382944878622No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA500.11526846024390805No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA500.11526846024390805No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG490.11296309103902989No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT490.11296309103902989No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT480.11065772183415175No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA480.11065772183415175No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA480.11065772183415175No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC480.11065772183415175No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC470.10835235262927358No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA460.10604698342439542No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT460.10604698342439542No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACC450.10374161421951725No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG450.10374161421951725No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACC440.10143624501463909No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)