FastQCFastQC Report
Sun 21 May 2023
EGAF00007848116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41918
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA20354.854716350970943No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13163.139462760627892No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11482.738680280547736No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA9252.206689250441338No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7511.7915931103583187No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT7361.7558089603511617No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6981.6651557803330312No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA6811.6246004103249203No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6591.5721169903144232No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6191.4766925902953385No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6191.4766925902953385No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT6151.46715015029343No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6101.4552221002910446No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA5741.369340140273868No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5551.3240135502648027No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5011.1951906102390382No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4871.1617920702323583No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4771.137935970227587No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4401.0496684002099337No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4351.037740350207548No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4271.0186554702037312No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4180.997184980199437No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4160.9924137601984828No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4130.9852569301970513No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4050.9661720501932344No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3900.9303879001860776No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3740.8922181401784437No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3690.880290090176058No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3690.880290090176058No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3670.8755188701751038No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3650.8707476501741496No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3450.8230354501646071No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3380.8063361801612672No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3340.7967937401593588No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3130.7466959301493391No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3060.7299966601459994No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2970.7085261701417053No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2910.6942125101388424No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2870.684670070136934No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2710.6465003101293001No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2680.6393434801278687No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2470.5892456701178491No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2470.5892456701178491No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2420.5773176201154635No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2390.5701607901140321No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2340.5582327401116466No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2220.529605420105921No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2200.5248342001049668No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2160.5152917601030583No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2120.5057493201011499No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2090.4985924900997185No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2030.4842788300968558No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1980.4723507800944701No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1970.469965170093993No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1900.45326590009065315No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1890.45088029009017605No Hit
TGTGCCTGTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1870.44610907008922185No Hit
TGTGCCTGTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1730.4127105300825421No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1730.4127105300825421No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1720.410324920082065No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1610.3840832100768167No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1610.3840832100768167No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1570.37454077007490816No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1550.3697695500739539No Hit
TGTGCCTGTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1500.3578415000715683No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1460.34829906006965977No Hit
TGTGCCTGTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1460.34829906006965977No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1450.34591345006918267No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1440.34352784006870557No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1440.34352784006870557No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1320.3149005200629801No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1260.30058686006011737No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1250.29820125005964027No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1210.28865881005773175No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1200.28627320005725465No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1160.2767307600553462No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1160.2767307600553462No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1130.2695739300539148No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT1120.2671883200534377No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1080.25764588005152916No Hit
TGTGCCTGTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1080.25764588005152916No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1070.25526027005105206No Hit
TGTGCCTGTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1070.25526027005105206No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1050.2504890500500978No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1010.24094661004818932No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT1000.23856100004771222No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT980.23378978004675796No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG970.2314041700462808No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA920.21947612004389522No Hit
TGTGCCTGTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT910.2170905100434181No Hit
TGTGCCTGTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT900.214704900042941No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT890.21231929004246383No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT850.2027768500405554No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT840.20039124004007824No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA830.19800563003960114No Hit
TGTGCCTGTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT830.19800563003960114No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA820.195620020039124No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.1932344100386469No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG800.19084880003816976No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC790.18846319003769263No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA790.18846319003769263No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT780.1860775800372155No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC770.1836919700367384No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA770.1836919700367384No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.18130636003626127No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA750.17892075003578414No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC750.17892075003578414No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG750.17892075003578414No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT740.17653514003530701No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC730.17414953003482989No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT710.16937831003387566No Hit
TGTGCCTGTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT690.1646070900329214No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA680.1622214800324443No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC640.1526790400305358No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA630.15029343003005868No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA630.15029343003005868No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCACGCT630.15029343003005868No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGATACCCACGCT620.14790782002958158No Hit
TGTGCCTGTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT610.14552221002910445No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC600.14313660002862733No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA600.14313660002862733No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA600.14313660002862733No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTTGATACCCACGCT590.1407509900281502No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC580.1383653800276731No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT570.13597977002719597No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA570.13597977002719597No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC560.13359416002671884No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA560.13359416002671884No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC550.1312085500262417No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT550.1312085500262417No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC540.12882294002576458No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.12643733002528748No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA520.12405172002481035No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTTGATACCCACGCT520.12405172002481035No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA490.11689489002337898No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG490.11689489002337898No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT490.11689489002337898No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA480.11450928002290185No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC480.11450928002290185No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA470.11212367002242474No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC470.11212367002242474No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT470.11212367002242474No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT470.11212367002242474No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA460.10973806002194761No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA460.10973806002194761No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC460.10973806002194761No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG450.1073524500214705No Hit
TGTGCCTGTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT450.1073524500214705No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT450.1073524500214705No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT440.10496684002099337No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT430.10258123002051625No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA430.10258123002051625No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT430.10258123002051625No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA420.10019562002003912No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC420.10019562002003912No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)