FastQCFastQC Report
Sun 21 May 2023
EGAF00007848118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47687
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA21284.462432109379915No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA21234.451947071528928No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA13462.8225721894856037No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12542.62964749302745No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT9672.0278063203808165No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA9061.8998888585987794No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA8771.8390756390630572No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT8591.8013295027995049No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7951.667121018306876No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7591.5916287457797722No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT7291.5287185186738523No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT6591.3819279887600395No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT6001.258204542118397No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5881.2330404512760291No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5721.1994883301528718No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA5631.180615262021096No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA5381.1281900727661627No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5301.111414012204584No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5281.1072199970641894No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5211.0925409440728082No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT5041.0568918153794535No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4500.9436534065887977No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4430.9289743535974165No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT4170.874452156772286No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4120.8639671189212992No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4080.8555790886405099No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3870.8115419296663661No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3800.7968628766749848No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3710.7779898085432088No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3710.7779898085432088No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3630.7612137479816302No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3600.7549227252710382No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3570.7486317025604462No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3500.733952649569065No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3480.7297586344286703No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3420.7171765890074863No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3390.7108855662968944No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3260.6836244678843291No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3180.6668484073227504No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2950.6186172332082119No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2910.6102292029274226No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2890.6060351877870279No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2860.5997441650764359No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2720.5703860590936733No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2720.5703860590936733No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2720.5703860590936733No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2680.5619980288128841No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2660.5578040136724893No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2420.5074758319877535No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2420.5074758319877535No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2350.4927967789963722No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2350.4927967789963722No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2290.4802147335751882No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2250.47182670329439885No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2160.452953635162623No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2150.4508566275924256No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2140.4487596200222283No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2070.43408056703084696No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2010.421498521609663No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1920.40262545347788703No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1830.3837523853461111No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1800.3774613626355191No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1800.3774613626355191No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1780.37326734749512447No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1730.3627823096441378No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1710.35858829450374313No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1680.3522972717931512No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1650.34600624908255917No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1600.3355212112315725No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1580.3313271960911779No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1570.32923018852098057No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1570.32923018852098057No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1500.31455113552959924No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1490.3124541279594019No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1430.29987208253821795No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1420.29777507496802064No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1380.2893870446872313No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1370.287290037117034No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1340.28099901440644204No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1300.2726109841256527No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1280.2684169689852581No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1200.2516409084236794No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1150.24115587057269278No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1140.23905886300249543No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1110.23276784029190345No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1020.21389477216012748No Hit
TACACATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1020.21389477216012748No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA980.20550674187933818No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA970.20340973430914086No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA940.19711871159854888No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA910.1908276888879569No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA910.1908276888879569No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT910.1908276888879569No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT880.18453666617736492No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA870.18243965860716757No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC840.1761486358965756No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT830.17405162832637824No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA810.1698576131859836No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT790.16566359804558894No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA790.16566359804558894No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT790.16566359804558894No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA780.16356659047539163No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT770.1614695829051943No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC750.15727556776479962No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC750.15727556776479962No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC740.1551785601946023No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT720.15098454505420764No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA720.15098454505420764No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC710.14888753748401032No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA710.14888753748401032No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC710.14888753748401032No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT690.14469352234361565No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT680.14259651477341834No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA680.14259651477341834No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT680.14259651477341834No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC680.14259651477341834No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA680.14259651477341834No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA660.13840249963302367No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA660.13840249963302367No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.13420848449262904No Hit
TACACATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT620.13001446935223435No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT620.13001446935223435No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA620.13001446935223435No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT610.12791746178203703No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA600.1258204542118397No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT600.1258204542118397No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA590.12372344664164238No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA580.12162643907144505No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.12162643907144505No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGGGCAAGCGTTA570.11952943150124772No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA570.11952943150124772No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.1174324239310504No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.1174324239310504No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA550.11533541636085307No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT530.11114140122045842No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.11114140122045842No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT530.11114140122045842No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT520.10904439365026107No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT520.10904439365026107No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT510.10694738608006374No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT510.10694738608006374No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA500.10485037850986642No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT480.10065636336947176No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT480.10065636336947176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)