FastQCFastQC Report
Sun 21 May 2023
EGAF00007848132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51676
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA46308.959671801223005No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA39267.5973372552055105No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA12462.411177335707098No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA11472.2195990401733883No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT11272.180896354206982No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA11142.155739608328818No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG11062.1402585339422555No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT10572.0454369533245607No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9651.8674045978790927No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA9451.8287019119126866No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9361.811285703227804No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8571.6584100936605No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG8421.6293830791856954No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT7281.408777769177181No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG7041.3623345460174936No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6281.2152643393451505No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6231.205588667853549No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6121.1843021905720257No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5951.1514049075005806No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT5881.1378589674123383No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT4860.9404752689836674No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT4370.8456536883659725No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT4260.8243672110844492No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4210.8146915395928477No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3980.7701834507314808No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3690.7140645560801919No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3640.7043888845885905No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3510.6792321387104265No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3400.6579456614289032No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3250.6289186469540986No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT3240.6269835126557783No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3140.6076321696725753No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2970.5747348866011301No Hit
TGTGCCTGTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2730.5282916634414428No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2440.4721727687901541No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2420.4683025001935135No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2420.4683025001935135No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2340.452821425806951No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2320.4489511572103104No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2220.4295998142271074No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2200.4257295456304667No Hit
TGTGCCTGTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT2180.42185927703382614No Hit
TGTGCCTGTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2110.408313336945584No Hit
TGTGCCTGTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT2070.4005727997523028No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2070.4005727997523028No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1930.3734809195758186No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1930.3734809195758186No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1910.369610650979178No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1820.35219444229429525No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1750.33864850220605314No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1690.3270376964161313No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1680.32510256211781097No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC1640.3173620249245298No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT1640.3173620249245298No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1630.31542689062620943No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1620.31349175632788917No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1600.30962148773124853No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1580.3057512191346079No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1510.29220527904636584No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1490.2883350104497252No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1470.28446474185308457No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1410.27285393606316277No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1410.27285393606316277No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1370.26511339886988156No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1360.26317826457156124No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1340.25930799597492066No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1340.25930799597492066No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1330.25737286167660034No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1310.25350259307995976No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC1290.24963232448331915No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT1270.24576205588667854No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1260.24382692158835823No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1210.23415125009675675No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1210.23415125009675675No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1210.23415125009675675No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1210.23415125009675675No Hit
TGTGCCTGTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1200.2322161157984364No Hit
TGTGCCTGTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1190.2302809815001161No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA1190.2302809815001161No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1130.2186701757101943No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1120.216735041411874No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA1100.21286477281523336No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1100.21286477281523336No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1100.21286477281523336No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1090.21092963851691307No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1090.21092963851691307No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1090.21092963851691307No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT1090.21092963851691307No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1080.20899450421859278No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1070.20705936992027246No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1070.20705936992027246No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.20318910132363188No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1040.20125396702531154No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1040.20125396702531154No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1010.19544856413035067No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC990.19157829553371003No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT950.18383775834042881No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC950.18383775834042881No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT930.17996748974378823No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC920.17803235544546792No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT920.17803235544546792No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT880.1702918182521867No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT870.16835668395386638No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA870.16835668395386638No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC850.1644864153572258No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCT850.1644864153572258No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA840.16255128105890548No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT840.16255128105890548No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT820.1586810124622649No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT810.15674587816394459No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC800.15481074386562427No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT800.15481074386562427No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT790.15287560956730395No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT780.15094047526898366No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT760.14707020667234305No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA750.14513507237402276No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA750.14513507237402276No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACC730.14126480377738215No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACC730.14126480377738215No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG720.13932966947906186No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCACGCT710.13739453518074154No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC710.13739453518074154No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG700.13545940088242123No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA690.13352426658410094No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACC680.13158913228578062No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT670.12965399798746033No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA650.12578372939081972No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCT650.12578372939081972No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC650.12578372939081972No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA640.12384859509249943No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC640.12384859509249943No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT630.12191346079417911No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT630.12191346079417911No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA630.12191346079417911No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA610.11804319219753852No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC600.1161080578992182No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCT600.1161080578992182No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA590.1141729236008979No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC590.1141729236008979No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC590.1141729236008979No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA580.1122377893025776No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCT580.1122377893025776No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.108367520705937No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG540.10449725210929639No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT540.10449725210929639No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACC540.10449725210929639No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT530.10256211781097609No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)