FastQCFastQC Report
Sun 21 May 2023
EGAF00007848133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51676
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA40527.841164176793869No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA36437.049694248780865No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT12822.4808421704466292No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT12552.428593544391981No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA11892.300874680702841No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT11182.163480145522099No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA10832.0957504450808884No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA10131.9602910441984673No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9371.8132208375261243No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9231.7861289573496402No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA9071.755166808576515No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8581.6603452279588204No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT8161.5790695874293676No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7821.5132750212864774No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7161.3855561575973372No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7101.3739453518074156No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6291.2171994736434708No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT6131.186237324870346No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6011.1630157132905024No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5571.077869804164409No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT5351.0352968496013624No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT5271.0198157752148No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT4090.7914699280130042No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3610.6985834816936295No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT3460.669556467218825No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT3430.663751064323864No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3430.663751064323864No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3380.6540753928322626No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3230.6250483783574581No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3080.5960213638826535No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3070.5940862295843331No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3040.5882808266893722No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3020.5844105580927317No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2900.561188946512888No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2580.4992646489666383No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2570.49732951466831793No Hit
TGTGCCTGTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2540.4915241117733571No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2360.4566916944035916No Hit
TGTGCCTGTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT2330.4508862915086307No Hit
TGTGCCTGTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT2320.4489511572103104No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2260.4373403514203886No Hit
TGTGCCTGTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2120.41024847124390434No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2120.41024847124390434No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2050.39670253115566223No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT2020.39089712826070133No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA2000.3870268596640607No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1970.3812214567690998No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT1970.3812214567690998No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1950.37735118817245916No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1860.35993497948757647No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1850.3579998451892561No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1730.3347782336094125No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1730.3347782336094125No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1680.32510256211781097No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1610.31155662202956885No Hit
TGTGCCTGTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1540.29801068194132674No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1530.2960755476430064No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1520.2941404133446861No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1520.2941404133446861No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1510.29220527904636584No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1500.2902701447480455No Hit
TGTGCCTGTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1500.2902701447480455No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1480.28639987615140494No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1480.28639987615140494No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA1450.280594473256444No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC1450.280594473256444No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1330.25737286167660034No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT1310.25350259307995976No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA1310.25350259307995976No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1310.25350259307995976No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1290.24963232448331915No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1260.24382692158835823No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1250.2418917872900379No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1240.23995665299171762No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1230.23802151869339733No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1220.23608638439507704No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1220.23608638439507704No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1210.23415125009675675No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1200.2322161157984364No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1180.2283458472017958No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1170.2264107129034755No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1150.22254044430683487No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1130.2186701757101943No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1120.216735041411874No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1120.216735041411874No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1110.2147999071135537No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1110.2147999071135537No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC1110.2147999071135537No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT1100.21286477281523336No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1100.21286477281523336No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1100.21286477281523336No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1090.21092963851691307No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1030.19931883272699125No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1020.19738369842867096No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1010.19544856413035067No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA990.19157829553371003No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA990.19157829553371003No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC990.19157829553371003No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT980.1896431612353897No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT940.18190262404210852No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT920.17803235544546792No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA910.17609722114714763No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC890.172226952550507No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC880.1702918182521867No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA870.16835668395386638No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT850.1644864153572258No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT830.1606161467605852No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCACGCT830.1606161467605852No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACC830.1606161467605852No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT820.1586810124622649No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA810.15674587816394459No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT810.15674587816394459No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC800.15481074386562427No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT790.15287560956730395No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA780.15094047526898366No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC760.14707020667234305No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA750.14513507237402276No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC740.14319993807570247No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT740.14319993807570247No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCT720.13932966947906186No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA720.13932966947906186No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA710.13739453518074154No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT710.13739453518074154No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT710.13739453518074154No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT710.13739453518074154No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT700.13545940088242123No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC680.13158913228578062No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCT680.13158913228578062No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT670.12965399798746033No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC660.12771886368914004No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCT650.12578372939081972No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC650.12578372939081972No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACC650.12578372939081972No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC630.12191346079417911No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACC620.11997832649585881No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC620.11997832649585881No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA610.11804319219753852No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT610.11804319219753852No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG610.11804319219753852No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.1161080578992182No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA600.1161080578992182No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.1161080578992182No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA590.1141729236008979No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG590.1141729236008979No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTTGATACCCGCACC590.1141729236008979No Hit
TGTGCCTGTCCGGACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCT580.1122377893025776No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA580.1122377893025776No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT580.1122377893025776No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT570.11030265500425729No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCT570.11030265500425729No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT560.108367520705937No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA560.108367520705937No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA550.10643238640761668No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCT540.10449725210929639No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA540.10449725210929639No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCT530.10256211781097609No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA520.10062698351265577No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)