FastQCFastQC Report
Sun 21 May 2023
EGAF00007848143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48704
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA20784.266590013140605No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA17343.5602825229960575No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG10682.1928383705650463No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8311.7062253613666227No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8271.6980124835742443No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8241.6918528252299607No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT8111.6651609724047307No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7981.6384691195795005No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7921.626149802890933No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT7801.6015111695137978No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7721.5850854139290407No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT7121.461892247043364No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6591.3530716162943495No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6431.320220105124836No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6301.2935282522996057No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA6141.260676741130092No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5991.2298784494086727No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5851.2011333771353483No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5651.160068988173456No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5401.1087385019710907No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5381.1046320630749014No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5331.0943659658344282No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5201.0676741130091985No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5061.0389290407358738No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5041.0348226018396847No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA4700.9650131406044679No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4690.9629599211563733No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4400.9034165571616295No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4320.8869908015768726No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4300.8828843626806834No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4180.8582457293035479No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4150.8520860709592641No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4020.8253942181340342No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3800.7802233902759528No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3770.7740637319316689No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3700.7596911957950067No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3540.7268396846254928No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3530.7247864651773982No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3390.6960413929040736No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3330.683722076215506No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3310.6796156373193166No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3200.657030223390276No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3180.6529237844940867No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3050.6262319316688567No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2980.6118593955321945No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2920.5995400788436268No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2910.5974868593955321No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2800.5749014454664915No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2690.5523160315374507No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2590.5317838370565046No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2580.5297306176084099No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2530.5194645203679369No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2370.48661300919842315No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2340.48045335085413926No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2310.4742936925098555No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2240.45992115637319314No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2230.45786793692509853No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2230.45786793692509853No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2220.45581471747700397No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2120.4352825229960578No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2080.42706964520367935No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2080.42706964520367935No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2080.42706964520367935No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2030.4168035479632063No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1820.37368593955321944No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1790.3675262812089356No Hit
TGTGCCTGTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1740.35726018396846254No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1620.3326215505913272No Hit
TGTGCCTGTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1600.328515111695138No Hit
TGTGCCTGTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1590.32646189224704336No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1560.3203022339027595No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1550.3182490144546649No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1540.3161957950065703No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1490.30592969776609724No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1460.2997700394218134No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1440.2956636005256242No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1390.2853975032851511No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1350.2771846254927727No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1350.2771846254927727No Hit
TGTGCCTGTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1320.27102496714848884No Hit
TGTGCCTGTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1310.2689717477003942No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1300.2669185282522996No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1290.26486530880420495No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1260.25870565045992117No Hit
TGTGCCTGTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1260.25870565045992117No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1230.25254599211563733No Hit
TGTGCCTGTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1180.24227989487516427No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1140.2340670170827858No Hit
TGTGCCTGTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1110.22790735873850199No Hit
TGTGCCTGTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1110.22790735873850199No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1040.21353482260183967No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1030.2114816031537451No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA990.20326872536136661No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG990.20326872536136661No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT980.20121550591327203No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT960.19710906701708278No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC960.19710906701708278No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC960.19710906701708278No Hit
TGTGCCTGTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT940.19300262812089355No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC900.18478975032851513No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA900.18478975032851513No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG870.17863009198423127No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA850.17452365308804205No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC830.17041721419185282No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.1663107752956636No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA780.16015111695137976No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT770.15809789750328515No Hit
TGTGCCTGTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT760.15604467805519054No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATGCGTAGGGTGCGAGCGTTA760.15604467805519054No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA750.15399145860709593No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT690.14167214191852825No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT690.14167214191852825No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC680.13961892247043364No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT670.13756570302233903No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC660.13551248357424442No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA660.13551248357424442No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT650.1334592641261498No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA650.1334592641261498No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC640.1314060446780552No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC640.1314060446780552No Hit
TGTGCCTGTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT640.1314060446780552No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA630.12935282522996058No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC630.12935282522996058No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA630.12935282522996058No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA620.12729960578186594No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA610.12524638633377136No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA610.12524638633377136No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT590.12113994743758214No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA590.12113994743758214No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT580.11908672798948752No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.11908672798948752No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT570.1170335085413929No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA570.1170335085413929No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA540.11087385019710908No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT540.11087385019710908No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT520.10676741130091984No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA520.10676741130091984No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA510.10471419185282523No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT510.10471419185282523No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA500.10266097240473063No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA490.10060775295663602No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA490.10060775295663602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)