FastQCFastQC Report
Sun 21 May 2023
EGAF00007848168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49647
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA20344.096924285455315No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA16913.4060466896287793No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG11172.2498841823272304No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11142.243841521139243No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8961.804741474812174No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG8691.750357524120289No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7991.6093620964005881No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7711.5529639253127079No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7001.4099542771970108No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6421.2931294942292586No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6391.2870868330412712No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6371.28305839224928No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT6071.222631780369408No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6051.2186033395774165No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6031.214574898785425No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5931.1944326948254678No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5921.1924184744294721No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5421.0917074546296857No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5231.0534372671057668No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5221.0514230467097712No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4900.9869679940379076No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4670.940640924930006No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4510.9084133985940742No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4410.8882711946341169No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4410.8882711946341169No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4370.8802143130501339No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4350.8761858722581425No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4260.8580578886941809No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4080.8218019215662578No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4050.8157592603782706No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3940.7936028360223175No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3780.7613753096863859No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3740.753318428102403No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3730.7513042077064073No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3670.7392188853304328No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3520.7090055793904969No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA3440.692891816222531No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3400.6848349346385482No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3360.6767780530545653No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3320.6687211714705823No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3310.6667069510745866No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3250.6546216286986122No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3100.6244083227586763No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3030.6103087799867062No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3000.6042661187987189No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2990.6022518984027232No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2940.5921807964227446No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2840.5720385924627873No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA2810.5659959312748001No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2750.5539106088988257No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2660.5357826253348641No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2590.5216830825628941No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2540.5116119805829153No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2460.49549821741494954No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2430.48945555622696235No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA2390.4813986746429794No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2380.47938445424698367No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2380.47938445424698367No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2370.47737023385098803No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2260.4552138094950349No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2070.4169436219711161No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2030.4088867403871332No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2000.402844079199146No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1930.3887445364271759No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1910.38471609563518444No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1850.37263077325921No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1830.3686023324672186No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1770.3565170100912442No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1770.3565170100912442No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1730.34846012850726127No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1680.33838902652728264No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1670.3363748061312869No Hit
TGTGCCTGTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1630.32831792454730396No Hit
TGTGCCTGTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1630.32831792454730396No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1630.32831792454730396No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1620.3263037041513082No Hit
TGTGCCTGTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1510.3041472797953552No Hit
TGTGCCTGTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1510.3041472797953552No Hit
TGTGCCTGTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1510.3041472797953552No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1500.30213305939935947No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1430.2880335166273894No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1430.2880335166273894No Hit
TGTGCCTGTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1430.2880335166273894No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1320.2658770922714363No Hit
TGTGCCTGTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1260.253791769895462No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1250.25177754949946624No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1240.2497633291034705No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1230.24774910870747477No Hit
TGTGCCTGTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1230.24774910870747477No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1220.24573488831147902No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1170.2356637863315004No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1110.223578463955526No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1110.223578463955526No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1070.21552158237154306No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1020.20545048039156444No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1000.201422039599573No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT980.1973935988075815No Hit
TGTGCCTGTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT960.19336515801559007No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA960.19336515801559007No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT950.19135093761959435No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA930.18732249682760288No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT920.18530827643160713No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT920.18530827643160713No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA920.18530827643160713No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG910.1832940560356114No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA900.1812798356396157No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC870.1752371744516285No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT860.17322295405563276No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA860.17322295405563276No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA840.16919451326364132No Hit
TGTGCCTGTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT830.16718029286764557No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA830.16718029286764557No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT810.1631518520756541No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.1631518520756541No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCATGCT780.15710919088766695No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT770.1550949704916712No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC770.1550949704916712No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA750.15106652969967974No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC740.14905230930368402No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC730.14703808890768827No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC720.14502386851169255No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT710.14300964811569683No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC710.14300964811569683No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT700.14099542771970108No Hit
TGTGCCTGTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT700.14099542771970108No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC690.13898120732370536No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT690.13898120732370536No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT670.1349527665317139No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT660.13293854613571815No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCATGCT650.13092432573972246No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA650.13092432573972246No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT640.1289101053437267No Hit
TGTGCCTGTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT630.126895884947731No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCAAGCGTTA610.12286744415573951No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA600.12085322375974378No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA600.12085322375974378No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA590.11883900336374807No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT590.11883900336374807No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.11682478296775234No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT570.1148105625717566No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC570.1148105625717566No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA560.11279634217576087No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA550.11078212177976514No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC540.1087679013837694No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA530.10675368098777369No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC530.10675368098777369No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC530.10675368098777369No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT520.10473946059177797No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC520.10473946059177797No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT520.10473946059177797No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA520.10473946059177797No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT520.10473946059177797No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT520.10473946059177797No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC520.10473946059177797No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT510.10272524019578222No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA500.1007110197997865No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA500.1007110197997865No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT500.1007110197997865No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)