FastQCFastQC Report
Sun 21 May 2023
EGAF00007848169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49647
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA19343.895502245855741No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA16403.303321449432997No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11202.2559268435152173No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG10412.096803432231555No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8511.7141015569923659No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG8381.6879166918444217No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7921.595262553628618No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7451.5005941950168187No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6921.393840514029045No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6911.3918262936330492No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6811.371684089673092No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6811.371684089673092No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6621.333413902149173No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5801.1682478296775232No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5631.134006082945596No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5621.1319918625496002No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5551.11789231977763No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5361.0796221322537112No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5181.0433661651257882No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5171.0413519447297923No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5171.0413519447297923No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5121.0312808427498137No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4550.9164702801780571No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4430.8922996354261084No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4420.8902854150301126No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4400.8862569742381211No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4320.8701432110701552No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4140.8338872439422322No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4130.8318730235462365No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4120.8298588031502407No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4010.8077023787942876No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3980.8016597176063005No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3920.789574395230326No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3870.7795032932503475No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3660.7372046649344371No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3570.7190766813704755No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3560.7170624609744799No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3490.7029629182025097No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3400.6848349346385482No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3330.670735391866578No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3290.6626785102825952No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA3110.626422543154672No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3090.6223941023626806No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3070.6183656615706891No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3060.6163514411746933No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3030.6103087799867062No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2930.5901665760267488No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2890.582109694442766No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2870.5780812536507746No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2730.5498821681068342No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA2670.5377968457308598No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2650.5337684049388683No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2600.5236973029588898No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA2600.5236973029588898No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2460.49549821741494954No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2420.4874413358309666No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2380.47938445424698367No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2370.47737023385098803No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2330.4693133522670051No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2300.46327069107901786No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2290.46125647068302217No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2250.45319958909903924No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2100.4229862831591033No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1940.39075875682317157No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1930.3887445364271759No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1930.3887445364271759No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1870.3766592140512015No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1820.3665881120712228No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1780.35853123048723995No Hit
TGTGCCTGTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1680.33838902652728264No Hit
TGTGCCTGTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1670.3363748061312869No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1660.33436058573529115No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1660.33436058573529115No Hit
TGTGCCTGTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1640.3303321449432997No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1580.3182468225673253No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1560.3142183817753339No Hit
TGTGCCTGTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1550.31220416137933815No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1490.3001188390033637No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1480.29810461860736803No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1470.2960903982113723No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1450.2920619574193808No Hit
TGTGCCTGTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1450.2920619574193808No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1400.28199085543940217No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1390.2799766350434065No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1300.2618486514794449No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1200.24170644751948756No Hit
TGTGCCTGTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1200.24170644751948756No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1190.23969222712349184No Hit
TGTGCCTGTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1190.23969222712349184No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1160.23364956593550468No Hit
TGTGCCTGTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1130.22760690474751746No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1100.22156424355953028No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1090.21955002316353453No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1070.21552158237154306No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1070.21552158237154306No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1060.21350736197554737No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1050.21149314157955165No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1040.20947892118355593No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1010.20343625999556872No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1000.201422039599573No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1000.201422039599573No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT990.19940781920357725No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA930.18732249682760288No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC900.1812798356396157No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA900.1812798356396157No Hit
TGTGCCTGTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT890.17926561524361997No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT870.1752371744516285No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG860.17322295405563276No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT830.16718029286764557No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC820.16516607247164986No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA790.15912341128366264No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT780.15710919088766695No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA770.1550949704916712No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT770.1550949704916712No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA760.15308075009567548No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT750.15106652969967974No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA750.15106652969967974No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT750.15106652969967974No Hit
TGTGCCTGTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT750.15106652969967974No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT740.14905230930368402No Hit
TGTGCCTGTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT730.14703808890768827No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT700.14099542771970108No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG700.14099542771970108No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA700.14099542771970108No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC690.13898120732370536No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCATGCT680.13696698692770962No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA670.1349527665317139No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC670.1349527665317139No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.1349527665317139No Hit
TGTGCCTGTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT660.13293854613571815No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT660.13293854613571815No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC660.13293854613571815No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC660.13293854613571815No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT660.13293854613571815No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA650.13092432573972246No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT640.1289101053437267No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT630.126895884947731No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.12085322375974378No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT580.11682478296775234No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT580.11682478296775234No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA570.1148105625717566No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA570.1148105625717566No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA570.1148105625717566No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC560.11279634217576087No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT550.11078212177976514No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG550.11078212177976514No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT540.1087679013837694No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.1087679013837694No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCATGCT530.10675368098777369No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT520.10473946059177797No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCT510.10272524019578222No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA500.1007110197997865No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA500.1007110197997865No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCATGCT500.1007110197997865No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)