FastQCFastQC Report
Sun 21 May 2023
EGAF00007848182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84168
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA34624.113202167094383No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA32763.8922155688622757No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA23732.81936127744511No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA23702.815796977473624No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA17852.120758483033932No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG15601.8534359851725122No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA15201.805911985552704No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG14841.763140385894877No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA13201.568291987453664No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA13011.5457180876342553No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA12251.45542248835662No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT11801.401957988784336No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT11691.3888888888888888No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG11581.3758197889934418No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT11461.3615625891074992No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA11391.3532458891740329No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG11231.3342362893261097No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA11151.3247314894021482No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT10851.289088489687292No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA10511.2486930900104554No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA8721.0360231917118146No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA8380.9956277920349776No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA8030.9540442923676457No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT7990.9492918924056648No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7860.9338465925292272No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT7290.8661248930710009No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA7100.8435509932515921No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT6970.8281056933751545No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6740.8007793935937648No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6300.7485029940119761No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT6150.7306814941545481No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT6050.718800494249596No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6050.718800494249596No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6010.7140480942876153No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT5890.6997908944016729No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT5490.6522668947818648No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5240.6225643950194848No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5170.6142476950860184No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5170.6142476950860184No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5080.6035547951715616No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4680.5560307955517536No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4530.5382092956943256No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4380.5203877958368976No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT4370.5191996958464025No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT4250.50494249596046No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT4140.49187339606501285No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT3960.4704875962360992No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT3810.45266609637867117No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT3800.451477996388176No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3660.4348445965212432No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3650.433656496530748No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT3610.4289040965687672No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3600.42771599657827203No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3570.4241516966067864No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC3560.42296359661629124No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA3520.4182111966543104No Hit
TGTGCCTGTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3500.41583499667332No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC3470.4122706967018344No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC3460.41108259671133923No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3460.41108259671133923No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA3340.3968253968253968No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3330.3956372968349016No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC3220.38256819693945443No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA3220.38256819693945443No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3170.3766276969869784No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3130.37187529702499766No Hit
TGTGCCTGTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT3080.36593479707252163No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT3060.3635585970915312No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3040.36118239711054084No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2940.34930139720558884No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2900.34454899724360805No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2840.33742039730063683No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2820.33504419731964646No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2810.3338560973291512No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2770.32910369736717043No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2740.3255393973956848No Hit
TGTGCCTGTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT2730.3243512974051896No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT2680.3184107974527136No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2590.30771789753825685No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC2590.30771789753825685No Hit
TGTGCCTGTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT2580.3065297975477616No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT2540.3017773975857808No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT2490.2958368976333048No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2440.28989639768082887No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC2420.2875201976998384No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT2380.28276779773785765No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2350.279203497766372No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC2220.26375819788993443No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC2170.2578176979374584No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT2160.2566295979469632No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2150.25544149795646803No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2140.25425339796597285No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT2110.2506890979944872No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT2050.243560498051516No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2030.2411842980705256No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC2020.23999619808003042No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1980.2352437981180496No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1970.2340556981275544No Hit
TGTGCCTGTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1930.22930329816557363No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1910.2269270981845832No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1900.225738998194088No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT1890.2245508982035928No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1800.21385799828913601No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT1800.21385799828913601No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCT1780.21148179830814562No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT1730.20554129835566962No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC1700.20197699838418404No Hit
TGTGCCTGTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1680.19960079840319359No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC1680.19960079840319359No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1680.19960079840319359No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1650.196036498431708No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT1640.19484839844121282No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCT1640.19484839844121282No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT1600.19009599847923203No Hit
TGTGCCTGTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1570.18653169850774642No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1500.17821499857428003No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC1440.1710863986313088No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1430.1698982986408136No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT1410.16752209865982323No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1400.166333998669328No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC1350.16039349871685202No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1350.16039349871685202No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC1340.1592053987263568No Hit
TGTGCCTGTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1340.1592053987263568No Hit
TGTGCCTGTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1330.1580172987358616No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1290.1532648987738808No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGATACCCACGCT1280.15207679878338562No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC1270.1508886987928904No Hit
TGTGCCTGTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1240.1473243988214048No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1230.14613629883090962No Hit
TGTGCCTGTCCGGACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCT1210.1437600988499192No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1190.14138389886892883No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT1170.1390076988879384No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1130.1342552989259576No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1120.1330671989354624No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTTGATACCCACGCT1100.130690998954472No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1080.1283147989734816No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTCGCTCCCCATGCT1080.1283147989734816No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1080.1283147989734816No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT1060.1259385989924912No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1050.124750499001996No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.124750499001996No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1020.12118619903051041No Hit
TGTGCCTGTCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT1020.12118619903051041No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTTGATACCCACGCT1010.11999809904001521No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT1010.11999809904001521No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACT980.1164337990685296No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCACGCT950.112869499097044No Hit
TGTGCCTGTCCGGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACC940.11168139910654881No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT940.11168139910654881No Hit
TGTGCCTGTCCGGACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCT930.11049329911605361No Hit
TGTGCCTGTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT920.10930519912555842No Hit
TGTGCCTGTCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC910.1081170991350632No Hit
TGTGCCTGTCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCT910.1081170991350632No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT900.10692899914456801No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACT880.1045527991635776No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC860.1021765991825872No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT850.10098849919209202No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT850.10098849919209202No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT850.10098849919209202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)