FastQCFastQC Report
Sun 21 May 2023
EGAF00007848185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61280
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA22783.717362924281984No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA21683.5378590078328984No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA14622.385770234986945No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13262.1638381201044385No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT9331.5225195822454307No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT8861.445822454308094No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT8781.4327676240208878No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA7831.2777415143603132No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7661.25No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7591.2385770234986946No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7531.2287859007832898No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7181.1716710182767625No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA7061.152088772845953No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7041.1488250652741514No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA7031.1471932114882506No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6981.1390339425587468No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6941.1325065274151436No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6741.099869451697128No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT6731.098237597911227No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6701.093342036553525No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT6341.0345953002610966No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6010.9807441253263707No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5460.8909921671018277No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5220.8518276762402089No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5150.8404046997389034No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA5030.820822454308094No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4990.8142950391644909No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4990.8142950391644909No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4820.7865535248041776No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4810.7849216710182768No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4740.7734986945169713No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4680.7637075718015666No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4660.760443864229765No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4410.7196475195822455No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4400.7180156657963447No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4350.7098563968668408No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4320.7049608355091384No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4310.7033289817232377No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4270.6968015665796344No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4260.6951697127937336No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3920.6396866840731071No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3760.6135770234986946No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3730.6086814621409922No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3580.5842036553524804No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3510.5727806788511749No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3440.5613577023498694No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3310.5401436031331592No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3150.5140339425587467No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3080.5026109660574413No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3000.489556135770235No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2960.4830287206266318No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2920.4765013054830287No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2840.46344647519582244No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2780.4536553524804177No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2710.4422323759791123No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2630.429177545691906No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2600.4242819843342037No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2550.41612271540469975No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2470.4030678851174935No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2400.39164490861618795No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2380.3883812010443864No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2220.3622715404699739No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2190.35737597911227154No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2180.35574412532637073No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2120.34595300261096606No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2120.34595300261096606No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT2110.34432114882506526No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT2040.3328981723237598No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2040.3328981723237598No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1810.29536553524804177No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1810.29536553524804177No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1810.29536553524804177No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1740.2839425587467363No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1740.2839425587467363No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1730.28231070496083555No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1710.27904699738903394No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1660.27088772845953No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT1630.2659921671018277No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1610.2627284595300261No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1590.25946475195822455No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1570.25620104438642294No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1550.25293733681462144No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1530.24967362924281983No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1520.24804177545691905No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1510.24640992167101827No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1480.2415143603133159No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1470.23988250652741513No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1450.23661879895561358No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1430.233355091383812No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1420.23172323759791122No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1410.23009138381201044No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1400.22845953002610966No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1390.22682767624020886No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1390.22682767624020886No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1370.2235639686684073No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1370.2235639686684073No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1310.2137728459530026No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1300.21214099216710186No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1240.2023498694516971No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1240.2023498694516971No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1240.2023498694516971No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1220.19908616187989556No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1220.19908616187989556No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1180.19255874673629242No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1160.18929503916449086No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1160.18929503916449086No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1150.18766318537859009No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1130.1843994778067885No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1120.18276762402088773No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1110.18113577023498695No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1090.17787206266318537No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1060.17297650130548303No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1060.17297650130548303No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT1050.17134464751958225No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1050.17134464751958225No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1040.16971279373368145No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1040.16971279373368145No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1040.16971279373368145No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1030.16808093994778067No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1030.16808093994778067No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1020.1664490861618799No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1010.16481723237597912No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1000.1631853785900783No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA950.1550261096605744No Hit
TACACATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT930.15176240208877284No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT930.15176240208877284No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA920.15013054830287206No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTA920.15013054830287206No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC900.14686684073107048No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC870.14197127937336815No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT870.14197127937336815No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA860.14033942558746734No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT860.14033942558746734No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAACACGTAGGGTGCGAGCGTTA860.14033942558746734No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT840.13707571801566582No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA830.135443864229765No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT820.13381201044386423No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA810.13218015665796343No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT810.13218015665796343No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAACACGTAGGGTGCGAGCGTTA810.13218015665796343No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT800.13054830287206268No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC790.12891644908616187No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT790.12891644908616187No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT770.12565274151436032No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC770.12565274151436032No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA770.12565274151436032No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.12402088772845953No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA750.12238903394255875No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT740.12075718015665796No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG730.11912532637075718No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA730.11912532637075718No Hit
TACACATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT710.11586161879895561No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAACACGTAGGGTGCGAGCGTTA710.11586161879895561No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG700.11422976501305483No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.11422976501305483No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC690.11259791122715404No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA690.11259791122715404No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA680.11096605744125326No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTA680.11096605744125326No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAACACGTAGGGTGCGAGCGTTA670.10933420365535247No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC660.1077023498694517No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACT650.10607049608355093No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT650.10607049608355093No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA650.10607049608355093No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.10443864229765012No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCACGCT640.10443864229765012No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT630.10280678851174935No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT620.10117493472584856No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGATACCCACGCT620.10117493472584856No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT620.10117493472584856No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT620.10117493472584856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)